Template:Kyoto/Notebook/Aug 16

From 2013.igem.org

(Difference between revisions)
(→Colony PCR)
(→Colony PCR)
Line 20: Line 20:
{| class="wikitable"
{| class="wikitable"
!Sample||base pair
!Sample||base pair
-
|-
 
-
|8/15 BBa_R0061(Plax)||344
 
|-
|-
|8/15 BBa_I13504(RBS-GFP-DT))(1)||1174
|8/15 BBa_I13504(RBS-GFP-DT))(1)||1174
Line 31: Line 29:
|8/15 BBa_I0462(RBS-luxR-DT)(2)||1174
|8/15 BBa_I0462(RBS-luxR-DT)(2)||1174
|-
|-
-
|8/15 BBa_K117000(lysis1)(1)||456
+
|8/15 BBa_K748002(lysis3)||1053
-
|-
+
-
|8/15 BBa_K117000(lysis1)(2)||456
+
-
|-
+
-
|8/15 BBa_K748002(lysis3)||997
+
-
|-
+
-
|8/15 BBa_B0015(DT)||443
+
-
|-
+
-
|8/15 BBa_I732020(RBS-laczα-DT)||703
+
|-
|-
|negative control||--
|negative control||--
Line 46: Line 36:
!PreDenature||Denature||Annealing||Extension||cycle
!PreDenature||Denature||Annealing||Extension||cycle
|-
|-
-
|　°C||　°C||　°C||　°C||--
+
|94°C||94°C||55°C||68°C||--
|-
|-
-
|（◎Д◎）　||　||　||　||
+
|5min||30s||30s||1min||30cycles
|}
|}
</div>
</div>
Line 55: Line 45:
!Sample||base pair
!Sample||base pair
|-
|-
-
|(｀・ω・´)||
+
|8/15 BBa_R0061(Plax)||344
 +
|-
 +
|8/15 BBa_K117000(lysis1)(1)||456
 +
|-
 +
|8/15 BBa_K117000(lysis1)(2)||456
 +
|-
 +
|8/15 BBa_B0015(DT)||443
 +
|-
 +
|8/15 BBa_I732020(RBS-laczα-DT)||703
 +
|-
 +
|negative control||--
|}
|}
{| class="wikitable"
{| class="wikitable"
!PreDenature||Denature||Annealing||Extension||cycle
!PreDenature||Denature||Annealing||Extension||cycle
|-
|-
-
|　&deg;C||　&deg;C||　&deg;C||　&deg;C||--
+
|94&deg;C||94&deg;C||55&deg;C||68&deg;C||--
|-
|-
-
|（◎Д◎）　||　||　||　||
+
|5min||30s||30s||40s||30cycles
|}
|}

Revision as of 14:02, 23 September 2013

Contents

Aug 16

8/15 BBa_R0061(Plax) BBa_I13504(RBS-GFP-DT))(1) BBa_I13504(RBS-GFP-DT)(2) BBa_I0462(RBS-luxR-DT)(1) BBa_I0462(RBS-luxR-DT)(2) BBa_K117000(lysis1)(1) BBa_K117000(lysis1)(2) BBa_K748002(lysis3)(1) BBa_K748002(lysis3)(2) BBa_B0015(DT) BBa_I732020(RBS-laczα-DT)


Colony PCR

A

Samplebase pair
8/15 BBa_I13504(RBS-GFP-DT))(1)1174
8/15 BBa_I13504(RBS-GFP-DT)(2)1174
8/15 BBa_I0462(RBS-luxR-DT)(1)1174
8/15 BBa_I0462(RBS-luxR-DT)(2)1174
8/15 BBa_K748002(lysis3)1053
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30cycles
Samplebase pair
8/15 BBa_R0061(Plax)344
8/15 BBa_K117000(lysis1)(1)456
8/15 BBa_K117000(lysis1)(2)456
8/15 BBa_B0015(DT)443
8/15 BBa_I732020(RBS-laczα-DT)703
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s40s30cycles

Master Plate

A

NumberUse LB plate (+抗生物質)
ʅ（´◔౪◔）ʃ

Transformation

A

NameSampleCompetent CellsTotalPlate
いでんしなんちゃらµLごにょごにょµLほにゃららµLこーせーぶっしつ
いかどうよう

Liquid Culture

A

Samplemedium
(・ω・)
（・Ｘ・） 　
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