Template:Kyoto/Notebook/Aug 17

From 2013.igem.org

(Difference between revisions)
(→Colony PCR)
(→Liquid Culture)
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</div>
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===Master Plate===
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<div class="experiment">
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<span class="author">Nakamoto</span>
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{| class="wikitable"
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!Number||Use LB plate (+CP)
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|-
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|1||BBa_K112806(lysis2) -(1)
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|}
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</div>
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<div class="experiment">
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{| class="wikitable"
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!Number||Use LB plate (+Kana)
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|-
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|1||BBa_Q04400(tetR) -(1)
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|}
</div>
</div>

Revision as of 14:08, 23 September 2013

Contents

Aug 17

Colony PCR

Nakamoto

Samplebase pair
8/15 BBa_K112806(lysis2) -(1)828
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30cycles

File:Image

Samplebase pair
8/10 BBa_Q0400 -(1)3995
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s30s30cycles

File:Image

Liquid Culture

Nakamoto

Samplemedium
8/15 BBa_K112806(lysis2) -(1)Plusgrow medium(+CP)
8/15 BBa_Q04400(tetR) -(1)Plusgrow medium(+Amp)

Master Plate

Nakamoto

NumberUse LB plate (+CP)
1BBa_K112806(lysis2) -(1)
NumberUse LB plate (+Kana)
1BBa_Q04400(tetR) -(1)
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