Template:Kyoto/Notebook/Aug 16

From 2013.igem.org

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<span class="author">A</span>
<span class="author">A</span>
{| class="wikitable"
{| class="wikitable"
-
!Number||Use LB plate (+抗生物質)
+
!Number||Use LB plate (+CP)
|-
|-
-
| ʅ（´◔౪◔）ʃ||
+
|1||BBa_I13504(RBS-GFP-DT))(1)
 +
|-
 +
|2||BBa_I13504(RBS-GFP-DT))(2)
 +
|-
 +
|3||BBa_K748002(lysis3)
 +
|-
 +
|4||BBa_R0061(Plux)
 +
|-
 +
|5||BBa_I0462(RBS-luxR-DT)(1)
 +
|-
 +
|6||BBa_I0462(RBS-luxR-DT)(2)
 +
|-
 +
|7||BBa_B0015(DT)
 +
|-
 +
|8||BBa_I732020(RBS-laczα-DT)
|}  
|}  
</div>
</div>
 +
===Transformation===
===Transformation===
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Revision as of 14:09, 23 September 2013

Contents

Aug 16

Colony PCR

A

Samplebase pair
8/15 BBa_I13504(RBS-GFP-DT))(1)1174
8/15 BBa_I13504(RBS-GFP-DT)(2)1174
8/15 BBa_I0462(RBS-luxR-DT)(1)1174
8/15 BBa_I0462(RBS-luxR-DT)(2)1174
8/15 BBa_K748002(lysis3)1053
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30cycles
Samplebase pair
8/15 BBa_R0061(Plax)344
8/15 BBa_K117000(lysis1)(1)456
8/15 BBa_K117000(lysis1)(2)456
8/15 BBa_B0015(DT)443
8/15 BBa_I732020(RBS-laczα-DT)703
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s40s30cycles

Master Plate

A

NumberUse LB plate (+CP)
1BBa_I13504(RBS-GFP-DT))(1)
2BBa_I13504(RBS-GFP-DT))(2)
3BBa_K748002(lysis3)
4BBa_R0061(Plux)
5BBa_I0462(RBS-luxR-DT)(1)
6BBa_I0462(RBS-luxR-DT)(2)
7BBa_B0015(DT)
8BBa_I732020(RBS-laczα-DT)

Transformation

A

NameSampleCompetent CellsTotalPlate
いでんしなんちゃらµLごにょごにょµLほにゃららµLこーせーぶっしつ
いかどうよう

Liquid Culture

A

Samplemedium
(・ω・)
（・Ｘ・） 　
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