Template:Kyoto/Notebook/Aug 16

From 2013.igem.org

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|4||BBa_R0061(Plux)
|4||BBa_R0061(Plux)
|-
|-
-
|5||BBa_I0462(RBS-luxR-DT)(1)
+
|5||BBa_K117000(lysis1)(1)
|-
|-
-
|6||BBa_I0462(RBS-luxR-DT)(2)
+
|6||BBa_K117000(lysis1)(2)
|-
|-
|7||BBa_B0015(DT)
|7||BBa_B0015(DT)
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|}  
</div>
</div>
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{| class="wikitable"
 +
!Number||Use LB plate (+Amp)
 +
|-
 +
|1||BBa_I0462(RBS-luxR-DT)(1)
 +
|-
 +
|2||BBa_I0462(RBS-luxR-DT)(2)
 +
|}
===Transformation===
===Transformation===

Revision as of 14:12, 23 September 2013

Contents

Aug 16

Colony PCR

A

Samplebase pair
8/15 BBa_I13504(RBS-GFP-DT))(1)1174
8/15 BBa_I13504(RBS-GFP-DT)(2)1174
8/15 BBa_I0462(RBS-luxR-DT)(1)1174
8/15 BBa_I0462(RBS-luxR-DT)(2)1174
8/15 BBa_K748002(lysis3)1053
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30cycles
Samplebase pair
8/15 BBa_R0061(Plax)344
8/15 BBa_K117000(lysis1)(1)456
8/15 BBa_K117000(lysis1)(2)456
8/15 BBa_B0015(DT)443
8/15 BBa_I732020(RBS-laczα-DT)703
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s40s30cycles

Master Plate

A

NumberUse LB plate (+CP)
1BBa_I13504(RBS-GFP-DT))(1)
2BBa_I13504(RBS-GFP-DT))(2)
3BBa_K748002(lysis3)
4BBa_R0061(Plux)
5BBa_K117000(lysis1)(1)
6BBa_K117000(lysis1)(2)
7BBa_B0015(DT)
8BBa_I732020(RBS-laczα-DT)
NumberUse LB plate (+Amp)
1BBa_I0462(RBS-luxR-DT)(1)
2BBa_I0462(RBS-luxR-DT)(2)

Transformation

A

NameSampleCompetent CellsTotalPlate
いでんしなんちゃらµLごにょごにょµLほにゃららµLこーせーぶっしつ
いかどうよう

Liquid Culture

A

Samplemedium
(・ω・)
（・Ｘ・） 　
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