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From 2013.igem.org

Revision as of 14:21, 23 September 2013 (view source) Akr (Talk | contribs) (→Colony PCR) ← Older edit		Revision as of 14:23, 23 September 2013 (view source) Akr (Talk | contribs) (→Liquid Culture) Newer edit →
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	!Sample||medium		!Sample||medium
	|-		|-
-	|(・ω・) ||	+	|8/15 BBa_I13504(RBS-GFP-DT))(1)||Plusgrow medium (+CP)
		+	|-
		+	|8/15 BBa_I13504(RBS-GFP-DT)(2)||Plusgrow medium (+CP)
		+	|-
		+	|8/15 BBa_I0462(RBS-luxR-DT)(2)||Plusgrow medium (+Amp)
		+	|-
		+	|8/15 BBa_K748002(lysis3)||Plusgrow medium (+CP)
		+	|-
		+	|8/15 BBa_R0061(Plux)||Plusgrow medium (+CP)
		+	|-
		+	|8/15 BBa_K117000(lysis1)(1)||Plusgrow medium (+CP)
		+	|-
		+	|8/15 BBa_K117000(lysis1)(2)||Plusgrow medium (+CP)
		+	|-
		+	|8/15 BBa_B0015(DT)||Plusgrow medium (+CP)
		+	|-
		+	|8/15 BBa_I732020(RBS-laczα-DT)||Plusgrow medium (+CP)
	|-		|-
-	|（・Ｘ・） ||
	|}		|}
	</div>		</div>

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Revision as of 14:23, 23 September 2013

Contents 1 Aug 16 1.1 Colony PCR 1.2 Master Plate 1.3 Liquid Culture

Aug 16

Colony PCR

Sample	base pair
8/15 BBa_R0061(Plux)	344
8/15 BBa_K117000(lysis1)(1)	456
8/15 BBa_K117000(lysis1)(2)	456
8/15 BBa_B0015(DT)	443
8/15 BBa_I732020(RBS-laczα-DT)	703
negative control	--

PreDenature	Denature	Annealing	Extension	cycle
94°C	94°C	55°C	68°C	--
5min	30s	30s	40s	30cycles

Master Plate

Number	Use LB plate (+Amp)
1	BBa_I0462(RBS-luxR-DT)(1)
2	BBa_I0462(RBS-luxR-DT)(2)

Liquid Culture