Template:Kyoto/Notebook/Sep 8

From 2013.igem.org

(Difference between revisions)
(→Sep 8)
(→Gel Extraction)
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|1||100bp ladder||
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|2||RBS-lysis1-DT||X+P
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|-
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|3|||RBS-lysis1-DT||X+P
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|-
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|5|||RBS-lysis3-DT||X+P
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|6|||RBS-lysis3-DT||X+P
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[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxbeforexx.xxx]]<br>
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!Lane||DNA||Enzyme
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|（＞ω・）b || ||
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|1||100bp ladder||
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|3||Pcun-pT181 atte||E+S
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|4||Pcun-pT181 atte||E+S
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|-
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|6||Spinach-DT||X+P
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|-
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|7||Spinach-DT||X+P
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|-
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|9||RBS-lacZα-DT||X+P
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|-
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|10||RBS-lacZα-DT||X+P
|}
|}
[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxbeforexx.xxx]]<br>
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!Lane||DNA||Enzyme
|-
|-
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|（＞ω・）b || ||
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|1||1kbp ladder||
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| || ||
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|2||Ptet-anti||E+P
 +
|-
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|3||Ptet-anti||E+P
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|-
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|5||pSB4K5||E+S
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|-
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|6||pSB4K5||E+S
|}
|}
[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxbeforexx.xxx]]<br>
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!Lane||DNA||Enzyme
!Lane||DNA||Enzyme
|-
|-
-
|（＞ω・）b || ||
+
|1||1kbp ladder||
 +
|-
 +
|2||Pcun-pT181 atte||S+P
 +
|-
 +
|3||Pcun-pT181 atte||S+P
 +
|-
 +
|5||Plac||S+P
 +
|-
 +
|6||Plac||S+P
 +
|-
 +
|8||pSB1C3||E+S
 +
|-
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|9||pSB1C3||E+S
 +
|-
 +
|11||pSB1C3||X+P
|-
|-
-
| || ||
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|12||pSB1C3||X+P
|}
|}
[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxbeforexx.xxx]]<br>
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|（ΦωΦ^）|| || || ||
|（ΦωΦ^）|| || || ||
|}
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===Ligation===
===Ligation===
   
   

Revision as of 17:34, 23 September 2013

Contents

Sep 8

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
1100bp ladder
2RBS-lysis1-DTX+P
3RBS-lysis1-DTX+P
5RBS-lysis3-DTX+P
6RBS-lysis3-DTX+P

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
1100bp ladder
3Pcun-pT181 atteE+S
4Pcun-pT181 atteE+S
6Spinach-DTX+P
7Spinach-DTX+P
9RBS-lacZα-DTX+P
10RBS-lacZα-DTX+P

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
11kbp ladder
2Ptet-antiE+P
3Ptet-antiE+P
5pSB4K5E+S
6pSB4K5E+S

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
11kbp ladder
2Pcun-pT181 atteS+P
3Pcun-pT181 atteS+P
5PlacS+P
6PlacS+P
8pSB1C3E+S
9pSB1C3E+S
11pSB1C3X+P
12pSB1C3X+P

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experimentnamexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL

Restriction Enzyme Digestion

じっけんしゃ

DNArestricion enzymerestriction enzymeBufferBSAMilliQtotal
2 cuts（°°） µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL

じっけんしゃ

DNArestricion enzymerestriction enzymeBufferBSAMilliQtotal
2 cuts（°°） µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL

Transformation

じっけんしゃ

NameSampleCompetent CellsTotalPlate
いでんしなんちゃらµLごにょごにょµLほにゃららµLこーせーぶっしつ
いかどうよう

Electrophoresis

じっけんしゃ

LaneSampleEnzyme1Enzyme2
（°Д°）
LaneSampleEnzyme1Enzyme2
（°Д°）
LaneSampleEnzyme1Enzyme2
（°Д°）

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experimentnamexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL
stateVectorInserterLigation High ver.2
experimentnamexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL

PCR

No name

genome DNAKOD plus10x bufferdNTPMgSO4SasA_fwd primerSasA_rev primerMilliQtotal
0.52.52.51.50.750.75 25
PreDenatureDenatureAnnealingExtensioncycle
　°C　°C　°C　°C--
（◎Д◎）　　　　

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
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