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Template:Kyoto/Notebook/Sep 8
From 2013.igem.org
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!DNA||concentration[µg/mL]||260/280||260/230 | !DNA||concentration[µg/mL]||260/280||260/230 | ||
|- | |- | ||
| - | | | + | |Pcon-pT181 anti||243.0||1.90||1.81 |
| + | |- | ||
| + | |Pcon-Spinach-DT||253.7||1.57||1.51 | ||
|} | |} | ||
| + | |||
===Ligation=== | ===Ligation=== | ||
Revision as of 17:54, 23 September 2013
Contents |
Sep 8
Gel Extraction
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | |
| 2 | RBS-lysis1-DT | X+P |
| 3 | RBS-lysis1-DT | X+P |
| 5 | RBS-lysis3-DT | X+P |
| 6 | RBS-lysis3-DT | X+P |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| RBS-lysis1-DT(X+P) | 5.3 | 1.98 | 0.06 | |
| RBS-lysis3-DT(X+P) | 5.3 | 1.98 | 0.06 |
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | |
| 3 | Pcon-pT181 atte | E+S |
| 4 | Pcon-pT181 atte | E+S |
| 6 | Spinach-DT | X+P |
| 7 | Spinach-DT | X+P |
| 9 | RBS-lacZα-DT | X+P |
| 10 | RBS-lacZα-DT | X+P |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| Pcon-pT181 atte(E+S) | 5.2 | 1.82 | 0.36 | |
| Spinach-DT(X+P) | 8.0 | 1.87 | 0.25 | |
| RBS-lacZα-DT(X+P) | 10.3 | 1.71 | 0.54 |
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 1kbp ladder | |
| 2 | Ptet-pT181-anti | E+P |
| 3 | Ptet-pT181-anti | E+P |
| 5 | pSB4K5 | E+S |
| 6 | pSB4K5 | E+S |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| Ptet-pT181-anti(E+P) | 30.6 | 1.84 | 1.16 | |
| pSB4K5(E+S) | 21.8 | 1.87 | 0.98 |
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 1kbp ladder | |
| 2 | Pcon-pT181 atte | S+P |
| 3 | Pcon-pT181 atte | S+P |
| 5 | Plac | S+P |
| 6 | Plac | S+P |
| 8 | pSB1C3 | E+S |
| 9 | pSB1C3 | E+S |
| 11 | pSB1C3 | X+P |
| 12 | pSB1C3 | X+P |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| Pcon-pT181 atte(S+P) | 14.5 | 1.91 | 1.09 | |
| Plac(S+P) | 11.6 | 1.74 | 1.19 | |
| pSB1C3(E+S) | 5.7 | 1.74 | 0.34 | |
| pSB1C3(X+P) | 5.6 | 2.00 | 0.37 |
Miniprep
| DNA | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| Pcon-pT181 anti | 243.0 | 1.90 | 1.81 |
| Pcon-Spinach-DT | 253.7 | 1.57 | 1.51 |
Ligation
| state | Vector | Inserter | Ligation High ver.2 | ||
|---|---|---|---|---|---|
| experiment | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
| NC | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
Restriction Enzyme Digestion
| DNA | restricion enzyme | restriction enzyme | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 2 cuts | （°°） µL | µL | µL | µL | µL | µL | µL |
| NC | µL | µL | µL | µL | µL | µL | µL |
| DNA | restricion enzyme | restriction enzyme | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 2 cuts | （°°） µL | µL | µL | µL | µL | µL | µL |
| NC | µL | µL | µL | µL | µL | µL | µL |
Transformation
| Name | Sample | Competent Cells | Total | Plate |
|---|---|---|---|---|
| いでんし | なんちゃらµL | ごにょごにょµL | ほにゃららµL | こーせーぶっしつ |
| いかどうよう | ||||
Electrophoresis
| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| （°Д°） |
| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| （°Д°） |
| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| （°Д°） |
Gel Extraction
| Lane | DNA | Enzyme |
|---|---|---|
| （＞ω・）b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
| Lane | DNA | Enzyme |
|---|---|---|
| （＞ω・）b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
| Lane | DNA | Enzyme |
|---|---|---|
| （＞ω・）b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
| Lane | DNA | Enzyme |
|---|---|---|
| （＞ω・）b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
| Lane | DNA | Enzyme |
|---|---|---|
| （＞ω・）b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
Ligation
| state | Vector | Inserter | Ligation High ver.2 | ||
|---|---|---|---|---|---|
| experiment | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
| NC | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
| state | Vector | Inserter | Ligation High ver.2 | ||
|---|---|---|---|---|---|
| experiment | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
| NC | name | xx µg/mL | name | xx µg/mL | xx µg/mL |
PCR
| genome DNA | KOD plus | 10x buffer | dNTP | MgSO4 | SasA_fwd primer | SasA_rev primer | MilliQ | total |
|---|---|---|---|---|---|---|---|---|
| 0.5 | 2.5 | 2.5 | 1.5 | 0.75 | 0.75 | 25 |
| PreDenature | Denature | Annealing | Extension | cycle |
|---|---|---|---|---|
| °C | °C | °C | °C | -- |
| （◎Д◎） |
Gel Extraction
| Lane | DNA | Enzyme |
|---|---|---|
| （＞ω・）b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
| Lane | DNA | Enzyme |
|---|---|---|
| （＞ω・）b | ||
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Name | quantity | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|---|
| （´ω｀） | ||||
| （ΦωΦ^） |
