Team:UT Dallas/week5

From 2013.igem.org

(Difference between revisions)

Latest revision as of 21:02, 23 September 2013

8/1

<a name="h.gdt42dr1sitx"></a>Miniprep

<a name="h.k16mhl32quoi"></a>i5-16-1 [Com B] 7.4

<a name="h.ykgo7uctfizd"></a>i5-16-2 [Com B] 5.4

<a name="h.b5syoehwe5sd"></a>i5-16-3[Com B] 13.7

<a name="h.mfxia9twmnj"></a>i5-16-4 [Scr] no growth

<a name="h.601z1zffypq"></a>i5-16-5 [Scr] 10.7

<a name="h.ycdqtoz6j1jh"></a>i5-16-6[Scr] 7.8

<a name="h.ajykp6mwqppf"></a>i5-16-7[pScr] 8.4

<a name="h.ne55wp3gkau3"></a>i5-16-8[pScr] 9.1

<a name="h.e13d54dyzft7"></a>i5-16-9[pScr] 10.6

<a name="h.txo346njagbb"></a>i5-16-10 [Cra] 19.9

<a name="h.c3473p5uadpe"></a>i5-16-11[Cra] 5.2

<a name="h.wbmb3g1s7ugf"></a>i5-16-12 [Cra] 6.4

<a name="h.kv0tfbo2jv8i"></a>i5-16-13[pFru] 15.3

<a name="h.j3t9j0u7xkkq"></a>i5-16-14[pFru] 5.4

<a name="h.ilxeqahhep0j"></a>i5-16-15[pFru] 5.3

<a name="h.iwp9ty6hlxs1"></a>i5-16-16[Com C] 2.4

<a name="h.n4f67hryzt17"></a>i5-16-17[Com C] 7.7

<a name="h.l8oo77q6fsr"></a>i5-16-18[Com C] 5.8

<a name="h.s0shikkagflk"></a>Concentrations too low

<a name="h.h0j00xjiuue9"></a>Redo digestion and ligation (Plan B)

<a name="h.1h2u7hs3ix23"></a>Digestion

<a name="h.nw7x4tp0kj95"></a>	<a name="h.b77riogep97k"></a>i5-11-5 (100.9 ng/ul) [pLac-RBS-GFP]
<a name="h.7x23o2q63rld"></a>DNA	<a name="h.xs95dkb0qv3g"></a>20
<a name="h.qmbaclu4and5"></a>NEBuffer	<a name="h.i4e84vgkrcxx"></a>5
<a name="h.9fr4omfw11ah"></a>EcoR	<a name="h.xp4lm573njg7"></a>1
<a name="h.fnra7wygpvwz"></a>PstI	<a name="h.k3078dha6squ"></a>1
<a name="h.uzdcyicnhuw5"></a>Water	<a name="h.peiv6cad45bh"></a>23
<a name="h.wla559qqazui"></a>Total	<a name="h.snx6esd4tjh1"></a>50 → i5-17-1

<a name="h.ak8khbywm050"></a>Digestion

<a name="h.plaqos35rnbu"></a>	<a name="h.oduap9peuhwz"></a>i4-45-3	<a name="h.28slfgkpyrku"></a>i4-45-2	<a name="h.qm7ywscmvd1o"></a>i5-11-5 [pLac-RBS-GFP]
<a name="h.2y6nakdykx4n"></a>DNA	<a name="h.nluimw5ad5ii"></a>20	<a name="h.euarp7r4qqr4"></a>20	<a name="h.u7pjsz33yp8u"></a>20
<a name="h.fnxqh2ibdalu"></a>NEBuffer	<a name="h.kme0h754mt8u"></a>5	<a name="h.pychv9g0t5aj"></a>5	<a name="h.90qha3jcqgai"></a>5
<a name="h.xp8k4bpokxpe"></a>EcoR	<a name="h.simmuu8727lv"></a>1	<a name="h.d4s682l63gvk"></a>1	<a name="h.g0zvv2bkjcot"></a>1
<a name="h.lctki6qvqypt"></a>PstI	<a name="h.mvrdl2k9tfm"></a>1	<a name="h.e1qgxndsr3jw"></a>1	<a name="h.pcbwtt3jlera"></a>1
<a name="h.kn30n1rxa7pm"></a>Water	<a name="h.hfipwz68ut8i"></a>23	<a name="h.9a1getpgeol4"></a>23	<a name="h.xfxc3bw7xdpb"></a>23
<a name="h.7ubx7vr86r15"></a>Total	<a name="h.2fqkbpkycpxr"></a>50	<a name="h.cd74i22n9ixv"></a>50	<a name="h.2thgvsmqsnes"></a>50

<a name="h.462qh4ufcpyq"></a>Nanodrop i4-45-2 18.4

<a name="h.qymzp02bzgjz"></a> i4-45-3 28.8

<a name="h.8yjdiim94mo2"></a>Ligation

<a name="h.odd5alle0l7u"></a>Mastermix: Vector: 16 Buffer: 16

<a name="h.750mg81plwxp"></a>Vector: i4-45-3

<a name="h.ko16dp6g3zlu"></a>Insert	<a name="h.g9oyoz6rhqne"></a>i5-13-9	<a name="h.ko16dp6g3zlu"></a>i5-13-9	<a name="h.ko16dp6g3zlu"></a>i5-13-10	<a name="h.g9oyoz6rhqne"></a>i5-13-11	<a name="h.hgf7vl6itpa4"></a>i5-13-12	<a name="h.ke9rrrsnsyde"></a>i5-13-13	<a name="h.1w7eymqeeklq"></a>i5-15-1
<a name="h.ldquz8vbyhe6"></a>	<a name="h.htbyr1ll042h"></a>3:1	<a name="h.qlmj2ypxhaup"></a>0:1	<a name="h.xb0vn1n1rtbt"></a>3:1	<a name="h.ws93oogubgzu"></a>3:1	<a name="h.n5puy2qf1f8"></a>3:1	<a name="h.oikpvcs0iovu"></a>3:1	<a name="h.1hj1ujr3oyqe"></a>3:1
<a name="h.gnq4j8mok285"></a>Insert	<a name="h.p7qkdz74u435"></a>7.5	<a name="h.do5uf7x3xfce"></a>0	<a name="h.gjadgexwrr8k"></a>6	<a name="h.qv4hhw4bo7xq"></a>1	<a name="h.dj2b0xeslc0r"></a>5	<a name="h.wxppe7l5ijoc"></a>1.5	<a name="h.4rr06z9x2bi2"></a>1
<a name="h.6nrozzpemgsl"></a>Vector	<a name="h.294k89lar7ku"></a>2	<a name="h.t20x9jisxas4"></a>2	<a name="h.ihz5jvqdk19p"></a>2	<a name="h.mlguz32wkiza"></a>2	<a name="h.thhdugjt5v1a"></a>2	<a name="h.6iohv0przw1"></a>2	<a name="h.t36qi49njmay"></a>2
<a name="h.ix5ibc2nn2bl"></a>Buffer	<a name="h.rbbw0rx8m512"></a>2	<a name="h.bg1sshpu7d3p"></a>2	<a name="h.dx7eibvymd2p"></a>2	<a name="h.ssu9fv79la3s"></a>2	<a name="h.nmchd34dvlrd"></a>2	<a name="h.o91ingua0t1m"></a>2	<a name="h.67y7n43zvoxa"></a>2
<a name="h.l9lmdir3u5kt"></a>Ligase	<a name="h.y303ooloe8eo"></a>1	<a name="h.f7r14f39zeo9"></a>1	<a name="h.3guxuh1ts81v"></a>1	<a name="h.fco4l7y7m9i0"></a>1	<a name="h.102mclq61u6e"></a>1	<a name="h.wlk08oxitdt"></a>1	<a name="h.f76a5efgbwy0"></a>1
<a name="h.hkl9t5ohw35f"></a>water	<a name="h.unws8s6zidez"></a>7.5	<a name="h.lot17dn25adf"></a>15	<a name="h.pjp8seiw7ctd"></a>9	<a name="h.2njbhf4869si"></a>14	<a name="h.azmz7p4j1n6j"></a>10	<a name="h.i79hrut5w68k"></a>13.5	<a name="h.kuwi6l8k42ow"></a>14
<a name="h.j2utgt1mtyc9"></a>Total	<a name="h.w0wf66iqe0tz"></a>20	<a name="h.7zdyt1ohkrtq"></a>20	<a name="h.l8d72odxorcw"></a>20	<a name="h.i3y4oas8rm7p"></a>20	<a name="h.jrhfboomo7ic"></a>20	<a name="h.7tkqll8rjzny"></a>20	<a name="h.7vhzegboowg7"></a>20

<a name="h.u3kbvtk7cxk"></a>1 2 3 4 5 6 7

<a name="h.vdlf9hfxs3za"></a>8/1

<a name="h.3kl77r7ego4"></a>Transformation

<a name="h.qnxw5fcp5bdl"></a>+ control → i5-10-4

<a name="h.d3hn90r6uogh"></a>1 → 10 colonies → picked 2 → i5-19-1

<a name="h.tky8lnz5box"></a> i5-19-2

<a name="h.v9a0276oa2c3"></a>2 →0 colonies

<a name="h.8rg3vllobj6l"></a>3 →0 colonies

<a name="h.terx6m36at4p"></a>4 →20 colonies → picked 2 → i5-19-3

<a name="h.ax82ddgvej52"></a> i5-19-4

<a name="h.kl8f3p7w9a94"></a>5 →50 + colonies → picked 2 → i5-19-5

<a name="h.rly6ll3758vh"></a> i5-19-6

<a name="h.t6yhc2bwlkmr"></a>6 →0 colonies (ruined plate)

<a name="h.txw92pj0umww"></a>7 →25 colonies → picked 2 → i5-19-7

<a name="h.2pg34ohcmptq"></a> i5-19-8

<a name="h.1yh2ap6r5dkj"></a>8/2

<a name="h.cbb9vyy27u50"></a>Transformation

<a name="h.tkzkibvuxed7"></a>+ control → i5-10-4

<a name="h.ygzhfj4d971e"></a>1 → 6 colonies → picked 2 → i5-19-9

<a name="h.jkleuycfz7gw"></a> i5-19-10

<a name="h.92goxt1940i6"></a>3 → 20 colonies → picked 2 → i5-19-11

<a name="h.n4keacizzoq7"></a> i5-19-12

<a name="h.1657iecoccwd"></a>6→ 8 colonies → picked 2 → i5-19-13

<a name="h.o4r84ljldi8j"></a> i5-19-14

<a name="h.hupfbsd3ivci"></a>8/3

<a name="h.h7xzg2fhesfu"></a>Miniprep

<a name="h.5324zw47di0h"></a>	<a name="h.i1tf65pvidmt"></a>Concentrations ng/ul
<a name="h.v7qvzht8rovt"></a>i5-19-1 [Com B]	<a name="h.oycn5t5zwqw"></a>90.3
<a name="h.yg0vhvy9xbin"></a>i5-19-2 [Com B]	<a name="h.6y6ofdr2i8h0"></a>77.5
<a name="h.ykmyu8l6hcn2"></a>i5-19-3 [pScr]	<a name="h.3mr1227qxaog"></a>39.6
<a name="h.psdo7yxrgz7u"></a>i5-19-4 [pScr]	<a name="h.d2gmy5rmmpa1"></a>89.5
<a name="h.whyjlta6ggiw"></a>i5-19-5 [Cra]	<a name="h.nvrasbi1dfp7"></a>71.6
<a name="h.2adme0o5ap57"></a>i5-19-6 [Cra]	<a name="h.jfs9zfjou67u"></a>120.2
<a name="h.g3ggqz1i48at"></a>i5-19-7 [Com C]	<a name="h.zieop85xawgl"></a>124.8
<a name="h.al7jvioy9ey8"></a>i5-19-8 [Com C]	<a name="h.qquo1hy4hm57"></a>101.6
<a name="h.sqlxlp2zkwfk"></a>i5-19-9	<a name="h.v2yehdjyl87j"></a>108.9
<a name="h.o2jm3ibgeqzn"></a>i5-19-10	<a name="h.r9qkczavx1zz"></a>102.3
<a name="h.3sd8ogw68ews"></a>i5-19-11 [Scr]	<a name="h.t04wha5b9m3c"></a>85.8
<a name="h.toa1hyt6erb"></a>i5-19-12	<a name="h.l2uogxg89tml"></a>86.9
<a name="h.xyabvc51wqmz"></a>i5-19-13 [pFru]	<a name="h.t6elcnx8txdu"></a>101.4
<a name="h.uwy9t1l2s2f1"></a>i5-19-14	<a name="h.zgdrxg3hnv3k"></a>97.8

<a name="h.oiv53yapn8kh"></a>Digestion	<a name="h.ou4hb7h0seu9"></a>i5-19-1	<a name="h.qte7npmt8r5c"></a>i5-19-2	<a name="h.t9s41xvjzho"></a>i5-19-3	<a name="h.40i1l3i78is1"></a>i5-19-4	<a name="h.br6zg9exyqka"></a>i5-19-5	<a name="h.ii3zqc2ors8j"></a>i5-19-6	<a name="h.fnvbqzaz7j1v"></a>i5-19-7	<a name="h.foy8blyca3l"></a>i5-19-8
<a name="h.rf3ds9ps6nzd"></a>DNA	<a name="h.zfo8ozunugrr"></a>4	<a name="h.68oznqb11yif"></a>4	<a name="h.1cme7yxrlqj6"></a>4	<a name="h.5l2uwmewg7ig"></a>4	<a name="h.58m87rj49d9u"></a>4	<a name="h.kvb2741eni0g"></a>4	<a name="h.lsdtxms32y8a"></a>4	<a name="h.c1b212wrflbg"></a>4
<a name="h.xikbro4ycyy1"></a>Buffer	<a name="h.vqsvtvp4raop"></a>1	<a name="h.jqxro38sinut"></a>1	<a name="h.2wn1xtn04gdc"></a>1	<a name="h.esnrwhx0uqms"></a>1	<a name="h.ug391abaxoi8"></a>1	<a name="h.4n9bhe83qr01"></a>1	<a name="h.v2f5x9dp0y5s"></a>1	<a name="h.905i5wpg00mj"></a>1
<a name="h.ihwa2erhrsb"></a>EcoR	<a name="h.41npynb4vjfh"></a>1	<a name="h.guc33o4xo1ny"></a>1	<a name="h.70bbc5qgkzwy"></a>1	<a name="h.m2ea79t163dd"></a>1	<a name="h.o4d1umcs55jw"></a>1	<a name="h.rl19xxze3i3y"></a>1	<a name="h.nh58fgbq8r36"></a>1	<a name="h.mi69vlcxfy8l"></a>1
<a name="h.mwpm9bcdyuz"></a>PstI	<a name="h.pbvtqamppw6t"></a>1	<a name="h.gbl8y6odfyfe"></a>1	<a name="h.8adi8qp99j8w"></a>1	<a name="h.260azt8lkiok"></a>1	<a name="h.41nqramq4vdp"></a>1	<a name="h.gad2myviufeq"></a>1	<a name="h.8cusr7ukuysz"></a>1	<a name="h.kiiioy1rzgw8"></a>1
<a name="h.41vrfxo8n7q1"></a>Water	<a name="h.dk5jox3vckg2"></a>3	<a name="h.a2vkupkprp96"></a>3	<a name="h.5dsl3xrxgwhv"></a>3	<a name="h.c860uhtzb5c"></a>3	<a name="h.iyx6d43bxlro"></a>3	<a name="h.meo2phn740du"></a>3	<a name="h.x8ehq55mafpt"></a>3	<a name="h.nw7dgbn47q03"></a>3
<a name="h.k9qik7fcg9bf"></a>Total	<a name="h.cul31bek5537"></a>10	<a name="h.f1xx1r7dzjjr"></a>10	<a name="h.ba6wq53qokt0"></a>10	<a name="h.41opoprgaair"></a>10	<a name="h.1ncbe75oart8"></a>10	<a name="h.txhacg7bmkcp"></a>10	<a name="h.ruryne7tthly"></a>10	<a name="h.ph7l6nwj0gjg"></a>10

<a name="h.w5r95sgzz3de"></a>Digestion #2	<a name="h.shg4jq7tk7e6"></a>1	<a name="h.i4tyvwlnt2cz"></a>2	<a name="h.4e8wcbhlw2uf"></a>3	<a name="h.yy6qr0cckv8x"></a>4	<a name="h.y2dm5eoypfl7"></a>5	<a name="h.ej3acoipgytk"></a>6	<a name="h.9c0kciymnagl"></a>7	<a name="h.waz255hd9f70"></a>8
<a name="h.25rke0gez3u"></a>DNA	<a name="h.d1o0e83sow7k"></a>4	<a name="h.g0jcy4txksbh"></a>4	<a name="h.sjk3vdrjgr0i"></a>4	<a name="h.s769fou19dqv"></a>4	<a name="h.wofmxmlpgegu"></a>4	<a name="h.490r9syon6yy"></a>4	<a name="h.uvj00hgmzve4"></a>4	<a name="h.yfgfb24q47wv"></a>4
<a name="h.yxfyh25kr"></a>Buffer	<a name="h.17jjqi4cko9w"></a>2	<a name="h.tbbryu1fptjw"></a>2	<a name="h.kil451gf68zb"></a>2	<a name="h.6bthosuis2ld"></a>2	<a name="h.5ruryk190l6y"></a>2	<a name="h.otjq8bsm9qzy"></a>2	<a name="h.p0coi219v6fu"></a>2	<a name="h.sxsz8pmiwxo4"></a>2
<a name="h.yo40h58dwy19"></a>EcoR	<a name="h.k66gxom0sihr"></a>1	<a name="h.adf4asiw7tc8"></a>1	<a name="h.3nnvtvje8bt3"></a>1	<a name="h.ml3rbpcgyxov"></a>1	<a name="h.kfp0hy18jq7t"></a>1	<a name="h.3bvz2np9788h"></a>1	<a name="h.xtmaj5gtpije"></a>1	<a name="h.oxd61apfk9qv"></a>1
<a name="h.irelmh3yosec"></a>PstI	<a name="h.s3uaas3bjpb0"></a>1	<a name="h.uo5q6iu6tjjc"></a>1	<a name="h.z9n2nm7tlijj"></a>1	<a name="h.ncu2jl8fqkt"></a>1	<a name="h.w1tglhjopah9"></a>1	<a name="h.lbrsjtd160he"></a>1	<a name="h.g7kckqak8syq"></a>1	<a name="h.4gkr4c91nlt2"></a>1
<a name="h.83pjd4a6h35b"></a>Water	<a name="h.36d3xu7uq87m"></a>12	<a name="h.8j42nnal03uu"></a>12	<a name="h.qleutk3745uj"></a>12	<a name="h.ymia2hk4ktkj"></a>12	<a name="h.xtg7amrq798x"></a>12	<a name="h.oe3428yq07hx"></a>12	<a name="h.i3rsk29jxrhj"></a>12	<a name="h.jsd4zv9zl3rh"></a>12
<a name="h.e6x30jr1n0q3"></a>Total	<a name="h.1vg74tppjqxe"></a>20	<a name="h.efr8py7hav4"></a>20	<a name="h.vz36gqyrwpjw"></a>20	<a name="h.gc3aj52yo3wh"></a>20	<a name="h.bbbs8h13lh35"></a>20	<a name="h.47r73jwcbtx4"></a>20	<a name="h.9gu3hni0qj2k"></a>20	<a name="h.rsfilo4bltd4"></a>20

<a name="h.luqq5le1czzf"></a>8/4

<a name="h.a5jijuql1rvr"></a>Digestion	<a name="h.ezp4ebrmrt24"></a>i5-19-9	<a name="h.iabe8cgq9hmu"></a>i5-19-10	<a name="h.7yg7kzgsqtkh"></a>i5-19-11	<a name="h.3q6b1wcbissm"></a>i5-19-12	<a name="h.k8ue8imb8tnj"></a>i5-19-13	<a name="h.ma78axahim7x"></a>i5-19-14
<a name="h.61t44pohvsaq"></a>DNA	<a name="h.x56fdk4v3ovw"></a>2	<a name="h.2hvapkiw4m87"></a>2	<a name="h.6f886gfut9m0"></a>2	<a name="h.h8oscj46xqwu"></a>2	<a name="h.kbbpg5ihzoll"></a>2	<a name="h.my8jpea79q93"></a>2
<a name="h.w9esfgelg75h"></a>Buffer	<a name="h.cfww47qpd4af"></a>1	<a name="h.qdl9k4lnp4su"></a>1	<a name="h.abiben5rcnr3"></a>1	<a name="h.3qcfojjf5lcr"></a>1	<a name="h.w7s2l12kuc50"></a>1	<a name="h.dzu83mp3t41"></a>1
<a name="h.af2xg2y7avl3"></a>EcoR	<a name="h.ni5uft1ulrul"></a>1	<a name="h.1wo6olhwpjw2"></a>1	<a name="h.wchoy4dr7x4g"></a>1	<a name="h.tbg88l1b6cy2"></a>1	<a name="h.c5y5gdkqyue2"></a>1	<a name="h.1am4iyn15gpa"></a>1
<a name="h.4tfklkehn3i5"></a>PstI	<a name="h.y5p3h0ubvj0y"></a>1	<a name="h.6i9m52boa53p"></a>1	<a name="h.13m0x4keo2uy"></a>1	<a name="h.yfvo3v5lne2u"></a>1	<a name="h.l32zngxtccv"></a>1	<a name="h.xiw4zxi2jl9m"></a>1
<a name="h.zi71ebctrsd"></a>Water	<a name="h.d0clbz3fd71r"></a>5	<a name="h.ryzpdn4j22ul"></a>5	<a name="h.gmgufotxnxut"></a>5	<a name="h.kp5bilx6wvit"></a>5	<a name="h.3vycwm7wlzip"></a>5	<a name="h.6c3d5q4bnsd1"></a>5
<a name="h.pfexr4rksv9i"></a>Total	<a name="h.xs9j65pjtnxv"></a>10	<a name="h.ayq5fs1mxbjc"></a>10	<a name="h.pk8u9by8feav"></a>10	<a name="h.aou9icqk61cv"></a>10	<a name="h.ez4mpw3kmvv0"></a>10	<a name="h.vfyzzhbb5ljt"></a>10

<a name="h.o6k1q9vzjd7a"></a>8/5

<a name="h.2jmyh9gl83yq"></a>Digestion

<a name="h.a6dix5iagyun"></a>	<a name="h.n9qgtg7uhhub"></a>i5-19-1	<a name="h.x3s5avd5kmni"></a>i5-19-3	<a name="h.4gq7ivf6m0v5"></a>i5-19-5	<a name="h.73crz0713h49"></a>i5-19-7	<a name="h.6nra6sudasj9"></a>i5-19-11	<a name="h.ao2ac858ohn1"></a>i5-19-13
<a name="h.ooejk4dkc05a"></a>DNA	<a name="h.wjvlwqn0s8dc"></a>5	<a name="h.eeg76xr9b7fv"></a>12.5	<a name="h.pfzeo3ypcsto"></a>7.1	<a name="h.qaoefpqrj4d7"></a>4	<a name="h.qu57tb5cdrok"></a>5.8	<a name="h.hgttgxoa6ab0"></a>5
<a name="h.e2razpc3mzde"></a>Buffer	<a name="h.f6uav9yzdyuy"></a>2	<a name="h.4aqyawqr5qv3"></a>2	<a name="h.b6rdcctzd01d"></a>2	<a name="h.g5zt6eo2s21p"></a>2	<a name="h.9skiq0maxx9b"></a>2	<a name="h.qk65utt0ptcm"></a>2
<a name="h.4bnct5urijfx"></a>EcoR	<a name="h.33oeogcp86tv"></a>1	<a name="h.jbie2odvtq7x"></a>1	<a name="h.xblh5q1t1us0"></a>1	<a name="h.hkms45pn6iwi"></a>1	<a name="h.3j60bs4b7afo"></a>1	<a name="h.l9onmyyqpe7z"></a>1
<a name="h.401yo1d7kmd3"></a>PstI	<a name="h.ifx7lnwalcxl"></a>1	<a name="h.bw9e2h26z0uf"></a>1	<a name="h.dnizgujui6pw"></a>1	<a name="h.x5v4qjvauhyc"></a>1	<a name="h.stev3lmw8nlg"></a>1	<a name="h.tt6o8acvxkx"></a>1
<a name="h.x6hciqcwfojq"></a>Water	<a name="h.9ht31r5zgfvd"></a>11	<a name="h.gv4kxpd8xtgw"></a>3.5	<a name="h.yp8bd29zeip0"></a>8.9	<a name="h.n372tyu3vdn2"></a>12	<a name="h.or1waswqa7s0"></a>10.2	<a name="h.26z1dl5b9lkj"></a>11
<a name="h.eho85b950qz4"></a>Total	<a name="h.yfnq9ah2mle0"></a>20	<a name="h.wqspntd4kqe0"></a>20	<a name="h.2jm6j51z1lg"></a>20	<a name="h.9q55arl12i8e"></a>20	<a name="h.wdg4qulbn5ru"></a>20	<a name="h.bgbd4xfuntjm"></a>20

<a name="h.a6dix5iagyun"></a>	<a name="h.n9qgtg7uhhub"></a>i5-19-1
<a name="h.ooejk4dkc05a"></a>DNA	<a name="h.wjvlwqn0s8dc"></a>5
<a name="h.e2razpc3mzde"></a>Buffer	<a name="h.f6uav9yzdyuy"></a>2
<a name="h.4bnct5urijfx"></a>EcoR	<a name="h.33oeogcp86tv"></a>1
<a name="h.401yo1d7kmd3"></a>PstI	<a name="h.ifx7lnwalcxl"></a>1
<a name="h.x6hciqcwfojq"></a>Water	<a name="h.9ht31r5zgfvd"></a>11
<a name="h.eho85b950qz4"></a>Total	<a name="h.yfnq9ah2mle0"></a>20

<a name="h.31al3sts3g4k"></a>Picked colonies from transformation of 8/2 and 8/1

<a name="h.fltmgetfd1cq"></a>Colonies from 8/1

<a name="h.ap81cglnf23i"></a>1→ i5-22-1 Com B

<a name="h.17hi9mgj55xx"></a>4→ i5-22-2 pScr Made glycerol stock for all 6

<a name="h.l5rilafwggo2"></a>5→ i5-22-3 Cra

<a name="h.4rt4e9ch9m8g"></a>7→ i5-22-4 Com C

<a name="h.mzbnml4va3h9"></a>Colonies from 8/2

<a name="h.bt2c6b5iji0t"></a>3 → i5-22-5 SCr

<a name="h.am7rhjrwfeew"></a>6→ i5-22-5 pFru

<a name="h.n85mmvxms0xm"></a>8/6

<a name="h.uhbw6a447c9c"></a>Miniprep Concentrations (ng/ul)

<a name="h.u1c3fa9lxf43"></a>i5-22-1 67.9 Com B

<a name="h.2s16kmp8fwjo"></a>i5-22-2 53.0 pScr

<a name="h.x2riupq5fzvg"></a>i5-22-3 90.9 Cra

<a name="h.3lehouxtgx4g"></a>i5-22-4 68.1 Com C

<a name="h.ghmduw89ynii"></a>i5-22-5 101.8 Scr

<a name="h.lj0ad7dpkd0a"></a>i5-22-6 185.5 pFru

<a name="h.2pzbef12bxzr"></a>Test Digestion

<a name="h.yb5evczhpuom"></a>	<a name="h.38w6pbrpqzdp"></a>i5-22-1	<a name="h.zgn0l6d6vb5t"></a>i5-22-2	<a name="h.r0yng41r0sic"></a>i5-22-3	<a name="h.p87wenjmpjwd"></a>i5-22-4	<a name="h.gkiou64ws2yz"></a>i5-22-5	<a name="h.v2g12flru3jf"></a>i5-22-6
<a name="h.va3lacp26cyj"></a>DNA	<a name="h.yg4cfww47xgo"></a>3	<a name="h.9tdm4gqjkkrq"></a>4	<a name="h.442vr9tpdgd"></a>2	<a name="h.o8mgufjbn5t0"></a>2.8	<a name="h.dt9091mp8qd3"></a>2	<a name="h.ulcrx6jqi4nf"></a>1
<a name="h.kcdjdapobt6l"></a>Cutsmart	<a name="h.elf3qphugt90"></a>2	<a name="h.3aenyfvkyno8"></a>2	<a name="h.ngwgkceddqu3"></a>2	<a name="h.vls4ubjauel4"></a>2	<a name="h.j3boni8avurg"></a>2	<a name="h.sofpjesu1oxp"></a>2
<a name="h.5ybhn2jvcnur"></a>EcoR-HF	<a name="h.85q2gdi4ggxr"></a>1	<a name="h.twfsbe5gdm9"></a>1	<a name="h.c1ss864a3wyk"></a>1	<a name="h.nmpu7yj3iswf"></a>1	<a name="h.h4x5nkmbjj16"></a>1	<a name="h.8wblq31iu8g7"></a>1
<a name="h.diecaj2350pk"></a>Spe I	<a name="h.431hxums39ls"></a>1	<a name="h.ub0o1kbmag9s"></a>1	<a name="h.bt4rjiep29yt"></a>1	<a name="h.z7prz5q61f4i"></a>1	<a name="h.5zwv242rkfvi"></a>1	<a name="h.5bb79vg9hwzd"></a>1
<a name="h.6gpw0rpjdfqg"></a>Water	<a name="h.tgamgczid50z"></a>13	<a name="h.g66iquhf4yb2"></a>12	<a name="h.daevk46emsi9"></a>14	<a name="h.5ctf9kjxkhwb"></a>13.2	<a name="h.chhppzctwrg1"></a>14	<a name="h.plsnqnkfaspd"></a>15
<a name="h.2r87y2owrqhc"></a>Total	<a name="h.cmo9e7puvjlm"></a>20	<a name="h.ag8h3mnqd2l7"></a>20	<a name="h.p4uocxac3rwe"></a>20	<a name="h.izmeh02q5kv7"></a>20	<a name="h.k5xtypjrck2a"></a>20	<a name="h.uyb7c1nwgy0u"></a>20

<a name="h.e4kpgf1mueqv"></a>Digestion of backbone (pLac-GFP i5-11-5)

<a name="h.d25ftzkasjyj"></a>	<a name="h.vwcs3u4w170g"></a>Com C (54.1)	<a name="h.c6xlvkt0pe77"></a>Com D (46.4)	<a name="h.o4fv70lu3990"></a>ComE (84.3)
<a name="h.44ngp9sc0gsx"></a>DNA	<a name="h.roe6ssa2ml6y"></a>40	<a name="h.fdpi22eu3m0i"></a>20	<a name="h.vg5gqoz8kjjv"></a>25
<a name="h.q0d68ztwehmr"></a>Buffer	<a name="h.v3t9mcfcbb2p"></a>5	<a name="h.dyjmwex93rs8"></a>5	<a name="h.9okzlob9xvmu"></a>5
<a name="h.nca0yzv523b"></a>Eco R	<a name="h.vh9t3cy5bmo6"></a>1	<a name="h.b6x14t79v1k4"></a>0	<a name="h.np51w6d9b29k"></a>0
<a name="h.5ago9jf3relr"></a>PstI	<a name="h.gqfk1jvl3ux7"></a>0	<a name="h.8d23ozny1z0m"></a>0	<a name="h.85nkqupm56uz"></a>1
<a name="h.tqxwofs4qxbc"></a>XbaI	<a name="h.dwkx341va5rr"></a>0	<a name="h.b43lcolhjx74"></a>1	<a name="h.5235tbrpkcom"></a>1
<a name="h.pznia856efr"></a>SpcI	<a name="h.hf4euicphgpb"></a>1	<a name="h.yu17ovg1nn2k"></a>1	<a name="h.sip7erk41zf6"></a>0
<a name="h.wnwxgm43end6"></a>Water	<a name="h.zdgligi8l7q8"></a>3	<a name="h.cukagcofwo2c"></a>23	<a name="h.xn42hpzcmfmz"></a>18
<a name="h.pvrd163duddt"></a>Total	<a name="h.i9ljeyx3xeak"></a>50	<a name="h.enwfnqcs2nbq"></a>50	<a name="h.j2h3yznwe43u"></a>50

<a name="h.y9wahg3dflx7"></a>Digestion of insert

<a name="h.uh8rvi2dflk4"></a>	<a name="h.tf68l84o8uvo"></a>Com A	<a name="h.nz13dv1nbx1n"></a>Com D	<a name="h.bnddbpky3te0"></a>Com E
<a name="h.44ngp9sc0gsx"></a>DNA	<a name="h.25pbpv9anmf5"></a>30	<a name="h.z0agsj6mnyww"></a>30	<a name="h.ssbk3twkauok"></a>30
<a name="h.q0d68ztwehmr"></a>Buffer	<a name="h.z5j53jq0ix0"></a>5	<a name="h.gbddpo6rdg2a"></a>5	<a name="h.y981msirfbq4"></a>5
<a name="h.nca0yzv523b"></a>Eco R	<a name="h.asxo1syv4hg1"></a>1	<a name="h.x71ph9ps5jlo"></a>0	<a name="h.caep0rvgrvh0"></a>0
<a name="h.5ago9jf3relr"></a>PstI	<a name="h.xxnp9vpfcyzi"></a>0	<a name="h.uh2ny991oqia"></a>0	<a name="h.ju4aq5ij8k44"></a>1
<a name="h.tqxwofs4qxbc"></a>XbaI	<a name="h.qihmsot6pl2a"></a>0	<a name="h.xtzo6ikfe7fq"></a>1	<a name="h.fje9ztr5fsb6"></a>1
<a name="h.pznia856efr"></a>SpcI	<a name="h.nxfafdazif22"></a>1	<a name="h.la1z3c7emzmo"></a>1	<a name="h.qceayaqmo7q1"></a>0
<a name="h.wnwxgm43end6"></a>Water	<a name="h.8ql6x1j0gvnk"></a>13	<a name="h.t9bpwap1kgw7"></a>13	<a name="h.7w9zite8hug9"></a>13
<a name="h.pvrd163duddt"></a>Total	<a name="h.659ckxaagez4"></a>50	<a name="h.ig2gsn60ly02"></a>50	<a name="h.ieswbupikysh"></a>50

<a name="h.db9jjmra7drh"></a>8/6

<a name="h.xq6o2p8t3ekh"></a>Constitutive promoters

<a name="h.wibnsic2a910"></a>CP Family member (strong)	<a name="h.6858tthab3pw"></a>BBa_J23119	<a name="h.x1z97dxzq6g7"></a>pSBIC3	<a name="h.gvhoulh69qzn"></a>2013 Kit plate 3	<a name="h.apwq70ggbn27"></a>18O	<a name="h.ints0anm9vhk"></a>i5-24-1
<a name="h.t0ps5t1x8q4l"></a>CP Family member (medium)	<a name="h.v56droy9k0d8"></a>BBa_K823014 (J23118)	<a name="h.82leoibdgaqw"></a>pSBIC3	<a name="h.ezuwrzapwbbw"></a>2013 Kit plate 1	<a name="h.3lqdqk898cbd"></a>22M	<a name="h.pek0wjix7k64"></a>i5-24-2
<a name="h.s5n956m48pp8"></a>CP Family member (weak)	<a name="h.3kc3i06t27zb"></a>BBa_K823012 (J23115)	<a name="h.jybz3h61sge6"></a>pSBIC3	<a name="h.h8fi7ukqrq2b"></a>2013 Kit plate 1	<a name="h.i92a4rin1bl2"></a>22I	<a name="h.o5q9bg9q4syf"></a>i5-24-3

<a name="h.rycve1ih3gur"></a>Transformation

<a name="h.3jrw5j6p2sgm"></a>+ control i5-10-4 → 3 colonies

<a name="h.adynbsnxormw"></a> i5-24-1 → 0 colonies

<a name="h.5rytshnqlc72"></a> i5-24-2 → 25 colonies

<a name="h.k7raf6akjbw7"></a> i5-24-3 → 15+ colonies

<a name="h.5tcllztdk398"></a>Gel Extraction

<a name="h.m0esxbrsc1wj"></a>	<a name="h.i9bylpzyxh2"></a>Concentration (ng/ul)
<a name="h.ints0anm9vhk"></a>i5-24-1 Com D	<a name="h.szs7o03b7gdz"></a>17.4
<a name="h.pek0wjix7k64"></a>i5-24-2 Com E	<a name="h.ecp3xchccta6"></a>7.6
<a name="h.o5q9bg9q4syf"></a>i5-24-3 ComA	<a name="h.37benhd42xmk"></a>7.9
<a name="h.ints0anm9vhk"></a>i5-24-4 BackA	<a name="h.ia9g6gxge9v6"></a>13.3
<a name="h.pek0wjix7k64"></a>i5-24-5 BackD	<a name="h.icjpxuqk2dxn"></a>11.6
<a name="h.o5q9bg9q4syf"></a>i5-24-6 BackE	<a name="h.x6jb2cbnabnt"></a>9.6

<a name="h.vh0iwdhh5ns7"></a>8/7

<a name="h.o7ul5ljt2ywf"></a>Inoculate from transformation 8/6

<a name="h.2mgh77d71y1j"></a>i5-24-2 → 25+ colonies → picked 1 colony → i5-25-7

<a name="h.62ewmx28f7mt"></a>i5-24-3 → 15+ colonies → picked 1 colony → i5-25-8

<a name="h.iqqfoukq2oz"></a>Com D vector backbone dephosphorilation

<a name="h.h8c9smgqz13j"></a>i5-25-5 → i5-25-9 12.5 ng/ul

<a name="h.8qibq2rdsosv"></a>Ligation

<a name="h.h7dmtux69b0h"></a>	<a name="h.75fcnjetnhp0"></a>Com A 3:1	<a name="h.ukmleeioa352"></a>Com A 0:1	<a name="h.g3ghsm5hssqk"></a>Com D 3:1	<a name="h.dnh0x7ywufq7"></a>Com D 0:1	<a name="h.tg789oqh35vb"></a>Com E 3:1	<a name="h.932dpd75v4u"></a>Com E 0:1
<a name="h.u5a3yt7rngho"></a>Insert	<a name="h.bj9gpbdmzw02"></a>10	<a name="h.u3k3id4m92o3"></a>0	<a name="h.7upxcedqi2ni"></a>6	<a name="h.ag8ouhvjt9pj"></a>0	<a name="h.xu0iinttgao0"></a>8	<a name="h.wavjmvuq0zyn"></a>0
<a name="h.a484e1vh4vez"></a>Vector	<a name="h.x5hvz11tyyru"></a>2	<a name="h.23wofbg7dk9o"></a>2	<a name="h.so5pbs3hicb"></a>5	<a name="h.3mxqleb9s8sn"></a>5	<a name="h.6zldclloa19r"></a>5	<a name="h.kgkhhly07n67"></a>5
<a name="h.fxjmuca5mbqo"></a>Buffer	<a name="h.684nr6u0aavk"></a>2	<a name="h.f29ne6k35ks0"></a>2	<a name="h.gwt9yiywu8g9"></a>2	<a name="h.9p1fp38hzmrf"></a>2	<a name="h.uw0txhnqieyp"></a>2	<a name="h.blp0k1q8ca3r"></a>2
<a name="h.eh8s8aai6ccl"></a>Ligase	<a name="h.rdx2fo9pjv50"></a>1	<a name="h.afzclqfltvst"></a>1	<a name="h.jn4ila6bgqp0"></a>1	<a name="h.niyq33tmnvsd"></a>1	<a name="h.mhdr3k2q70c4"></a>1	<a name="h.kdwsnfeco08e"></a>1
<a name="h.jhs7yvjkgipu"></a>Water	<a name="h.98uffvxft24t"></a>5	<a name="h.jx2mq28ndrhq"></a>15	<a name="h.avvmnx6rvcq7"></a>6	<a name="h.jomrcq1rkgq3"></a>12	<a name="h.2t6bgg8pq62k"></a>4	<a name="h.bk5em5zaqqk1"></a>12
<a name="h.r4gakjxirnm"></a>Total	<a name="h.b7px5p629wb2"></a>20	<a name="h.dr5wsphwugfr"></a>20	<a name="h.1jukh4az3av"></a>20	<a name="h.lv58iogydtel"></a>20	<a name="h.rs9niqua7llp"></a>20	<a name="h.kjbbjk1msd6u"></a>20

<a name="h.2j6yxbeu2tab"></a> insert → i5-23-3 i5-25-1 i5-25-2

<a name="h.3o8psi3w6gw"></a> Vector → i5-25-4 i5-25-9 i5-25-6

<a name="h.5yfxj9jlbxfe"></a> 1 2 3 4 5 6

<a name="h.42bedh5ney9q"></a>Transformation

<a name="h.sn6yo85y7wf5"></a>i5-25-1 Com A 3:1 → 0 colonies

<a name="h.cl44wfisntbw"></a>i5-25-2 Com A 0:1 → 0 colonies

<a name="h.xgujaa4m9ze6"></a>i5-25-3 Com D 3:1 → 0 colonies

<a name="h.lu7vdyxwppfl"></a>i5-25-4 Com D 0:1 → 0 colonies

<a name="h.3kh89f44w1zf"></a>i5-25-9 Com E 3:1 →20 colonies → picked 3 colonies → i5-25-10

<a name="h.iehtfwff6p7f"></a> → i5-25-11

<a name="h.5sydh01237my"></a> → - control i5-25-12

<a name="h.vdvi3f1vqpef"></a>i5-25-6 Com E 0:1 → 0 colonies

<a name="h.7q6omna9ogct"></a>i5-11-5 + colonies → plenty colonies

<a name="h.86c253es1cm9"></a>8/8

<a name="h.maa5grwkkatz"></a>Made glycerol stock from i5-15-7 and i5-25-8

<a name="h.ck7tfh7i7pmt"></a>Miniprep

<a name="h.3jpbcjkctjf6"></a>	<a name="h.qs5drvo28rm7"></a>Concentration (ng/ul)
<a name="h.t0j5ex3dratj"></a>i5-25-7 (medium CP)	<a name="h.91gep83p0uur"></a>109.3
<a name="h.cutho5fp38n3"></a>i5-25-8 (small CP)	<a name="h.kxapg9r7ojwl"></a>116.7

<a name="h.bvnzeytbhsfd"></a>Transformation

<a name="h.wg6vhgd3o65d"></a>i5-26-1 ComA 3:1

<a name="h.h76705w0uaeb"></a>i5-26-2 ComD 3:1

<a name="h.r92f30awsl43"></a>i5-26-3 pLac-GFP (+ control)

<a name="h.3dlfciq4c227"></a>i5-26-4 ComE 3:1

<a name="h.s3iv9m4193k7"></a>Digestion

<a name="h.1ybxzsmmqky1"></a>	<a name="h.cztt9ea1hy78"></a>i5-26-4	<a name="h.jxlyl7ugm2hk"></a>i5-26-5	<a name="h.12pcqlm112un"></a>i5-26-6	<a name="h.wk9jf1mpv9w8"></a>i5-26-7
<a name="h.evm95gi6jtb1"></a>DNA	<a name="h.xzp8jpql5w90"></a>30	<a name="h.43vqez2vhz75"></a>11	<a name="h.vsidxwd6b7mg"></a>20	<a name="h.yxlmuzunsphy"></a>20
<a name="h.11w80jnnzisx"></a>Buffer	<a name="h.op2rau3vwoi1"></a>5	<a name="h.u5r2g4aik22j"></a>5	<a name="h.93b535wzow5k"></a>5	<a name="h.2ohgeht0b18a"></a>5
<a name="h.48w8qfo4euj6"></a>PstI	<a name="h.5pgl0skigbuo"></a>1	<a name="h.r168jkjs4pk"></a>1	<a name="h.4djrpkwjmoyu"></a>1	<a name="h.cdxwrql0am71"></a>1
<a name="h.h6zo64dmnvbu"></a>SpcI	<a name="h.pedgoamanh83"></a>1	<a name="h.bano6kt8m2br"></a>1	<a name="h.df63q63ruwu3"></a>1	<a name="h.4rgl5cj93v1p"></a>1
<a name="h.veh91msc06en"></a>Water	<a name="h.damc93mc41p6"></a>13	<a name="h.4ss3yymvo82g"></a>32	<a name="h.7uzwotz35pb0"></a>23	<a name="h.2njg98ojeu4w"></a>23
<a name="h.iwuuy5rg7fcj"></a>Total	<a name="h.82wh9ptmr0k7"></a>50	<a name="h.lzlefu8t5gkh"></a>50	<a name="h.qy4uj2gg7gii"></a>50	<a name="h.6fj46w7i9aw9"></a>50

<a name="h.7ohb181cvlc5"></a>	<a name="h.1ndnlsrexn6p"></a>i5-26-8	<a name="h.yvwbj81t7251"></a>i5-26-9	<a name="h.4z2y4vcanl7x"></a>i5-26-10
<a name="h.bb6yew8hwcb5"></a>DNA	<a name="h.tcf5ai7nvoqi"></a>20	<a name="h.30ln3nwv5bi0"></a>20	<a name="h.dbwm952yixl0"></a>20
<a name="h.u3c5408277v5"></a>Buffer	<a name="h.4k8p8j95ks0u"></a>5	<a name="h.asyhjt5797kh"></a>5	<a name="h.u0pcfq9fffqb"></a>5
<a name="h.tqq52md11g1r"></a>PstI	<a name="h.gqx5h1gwtiph"></a>1	<a name="h.8gr973v6w2ij"></a>1	<a name="h.j8dbj5ybpdft"></a>1
<a name="h.o2auivle7b2"></a>XbaI	<a name="h.s65sqjtdl1fv"></a>1	<a name="h.de99c0rajslu"></a>1	<a name="h.gso7obnhep95"></a>1
<a name="h.aop280y95qcn"></a>Water	<a name="h.bsebvjwtdzro"></a>23	<a name="h.o5xzj7daaev0"></a>23	<a name="h.2raz43srotx"></a>23
<a name="h.xy9f3ac7jb3n"></a>Total	<a name="h.z39vfuuiv370"></a>50	<a name="h.ce8zmsqe95xj"></a>50	<a name="h.6p64omi9z28m"></a>50

<a name="h.cpb6f9oy218h"></a>Transformation

<a name="h.82l5yurfhh46"></a>3:1 ComA

<a name="h.zc9adneqyswj"></a>3:1 ComD

<a name="h.u4z7e8tb0rrs"></a>3:1 ComE

<a name="h.ib77a1l87t48"></a>+ control (pLac-GFP)

<a name="h.f00tibgergp"></a>8/9

<a name="h.kodcs5othrdp"></a>Ligation

<a name="h.z0kwt5nxopux"></a>insert: i5-26-10 Vector i5-26-5

<a name="h.7vluc54wk01l"></a>	<a name="h.k6mui7b6rz68"></a>3:1	<a name="h.dgvrvsc7bu0g"></a>0:1
<a name="h.oub71i2pw3n2"></a>Insert	<a name="h.amz5pvvj9q3t"></a>6.3	<a name="h.g3hp3ac60ptf"></a>0
<a name="h.ba6duxxfy39o"></a>Vector	<a name="h.60oo94a93h0b"></a>2	<a name="h.n9l5xk276hqv"></a>2
<a name="h.yly4vsjehj4"></a>Buffer	<a name="h.m9ba0nwqm5pd"></a>2	<a name="h.z71k2r76mt7f"></a>2
<a name="h.abw7saw8owjz"></a>Ligase	<a name="h.q3wgcgmtgmn4"></a>1	<a name="h.6dxg7vck2gbh"></a>1
<a name="h.erqt6icosda0"></a>Water	<a name="h.jl1ceixsbhx7"></a>8.7	<a name="h.gfwp6asmt3ku"></a>15
<a name="h.3ufubvxg739z"></a>Total	<a name="h.l6j7c6inlwdf"></a>20	<a name="h.zdtgvwkzd1kj"></a>20

<a name="h.68jcggnxe2kk"></a> 1 2

<a name="h.13k2y8znsdx"></a>Insert i5-26-10 Vector i5-25-4

<a name="h.peun7r6p3on6"></a>	<a name="h.asit7cs2rk80"></a>3:1	<a name="h.xgvt5agishz"></a>0:1
<a name="h.oub71i2pw3n2"></a>Insert	<a name="h.xt55nt6qk32q"></a>6.6	<a name="h.3vknl45azwvf"></a>0
<a name="h.ba6duxxfy39o"></a>Vector	<a name="h.d392mwaeapid"></a>2.5	<a name="h.vt7xt8wbfrnz"></a>2.5
<a name="h.yly4vsjehj4"></a>Buffer	<a name="h.8gz1siehn7ls"></a>2	<a name="h.m5w9b5bldgpf"></a>2
<a name="h.abw7saw8owjz"></a>Ligase	<a name="h.7dv0ve66io28"></a>1	<a name="h.e8rwfes5l0e6"></a>1
<a name="h.erqt6icosda0"></a>Water	<a name="h.ygv2qmektbul"></a>7.3	<a name="h.nwq9epaajli6"></a>14.5
<a name="h.3ufubvxg739z"></a>Total	<a name="h.k4b8pbveipsm"></a>20	<a name="h.9a8kvehi89fe"></a>20

<a name="h.ssbodvtory9t"></a> 3 4

<a name="h.l4rs6e81pgwk"></a>Insert i5-26-8 Vector i5-26-6

<a name="h.o14kiavtq1a6"></a>	<a name="h.2p4wl2lj83k8"></a>3:1	<a name="h.1s8hl78y6siy"></a>0:1
<a name="h.oub71i2pw3n2"></a>Insert	<a name="h.rsxvxg7ie1av"></a>4.36	<a name="h.q6l6h93wnemx"></a>0
<a name="h.ba6duxxfy39o"></a>Vector	<a name="h.xhwradv7ofd0"></a>2	<a name="h.hqbqsoydztdo"></a>2
<a name="h.yly4vsjehj4"></a>Buffer	<a name="h.5uydunqvx82s"></a>2	<a name="h.lg03ux2ir8a3"></a>2
<a name="h.abw7saw8owjz"></a>Ligase	<a name="h.963w6glbch7w"></a>1	<a name="h.avgzsiyct428"></a>1
<a name="h.erqt6icosda0"></a>Water	<a name="h.mn8l1ghufu4q"></a>10.64	<a name="h.fip39ewf67qm"></a>15
<a name="h.3ufubvxg739z"></a>Total	<a name="h.oz0oalujao1s"></a>20	<a name="h.qt6t1rqi4nbf"></a>20

<a name="h.cewwqq7q8bj1"></a> 5 6

<a name="h.4dvgy01tjgh8"></a>insert i5-26-9 Vector i5-26-6

<a name="h.uk8xljkz43q0"></a>	<a name="h.rm2a91ypwctm"></a>3:1	<a name="h.rlch06w846tp"></a>0:1
<a name="h.u924bruxam85"></a>insert	<a name="h.2bhmpi28qiop"></a>2.3	<a name="h.v61xzojcboi6"></a>0
<a name="h.fdocnt4y7ji3"></a>vector	<a name="h.sjk306r40m4u"></a>2	<a name="h.se475ae81qyn"></a>2
<a name="h.t4a7k41jiwj"></a>buffer	<a name="h.o3qtri6whr8e"></a>2	<a name="h.e5p264vlll3s"></a>2
<a name="h.7cppxveawh2b"></a>ligase	<a name="h.a29fc4w34toq"></a>1	<a name="h.e99nn4f6ts9w"></a>1
<a name="h.ob97c8cj4okf"></a>water	<a name="h.ze8koa8orksh"></a>12.7	<a name="h.f77rwsefozr5"></a>15
<a name="h.1qxb72m36rfj"></a>total	<a name="h.8b6ffl6k30mh"></a>20	<a name="h.chvq5gaw562h"></a>20

<a name="h.5h8su0c8cvoe"></a> 7 8

<a name="h.31elyj5r49k7"></a>Transformation

<a name="h.v1z8vn6xu3il"></a>Control (w/ GFP) [i5-10-1] → i5-28-1

<a name="h.qakg30ck28x3"></a>Control (w/out GFP) [i5-22-2] → i5-28-2

<a name="h.tz9j1wbg7mr3"></a>1 → i5-28-3

<a name="h.8il6mtefdzrt"></a>2 → i5-28-4

<a name="h.747dg3btie74"></a>3 → i5-28-5

<a name="h.9540h4uswcxa"></a>4 → i5-28-6 no growth

<a name="h.lrkw3udcpxuo"></a>5 → i5-28-7

<a name="h.58jyy6t51cy7"></a>6 → i5-28-8

<a name="h.vwu0q7nzmq1k"></a>7→ i5-28-9

<a name="h.2zsv5y78rfix"></a>8 → i5-28-10

<a name="h.73fhqn2jb5kj"></a>Ligation

<a name="h.g3cakqv1n2j"></a>redo from 8/7

<a name="h.5qyjxe42jdd2"></a>Transformation

<a name="h.te5asa59w3uc"></a>ComA 3:1

<a name="h.49y1cm5hrwwa"></a>Com A 0:1

<a name="h.4m8d55jvn825"></a>Com D 3:1

<a name="h.4afpflrq2tlb"></a>Com D 0:1

<a name="h.6s97dwn6g7av"></a>ComE 3:1

<a name="h.euuof7rbjt5n"></a>Com E 0:1

<a name="h.1e00fxu6rigc"></a>pLac-GFP as + control

<a name="h.8asdz9ocehq7"></a>8/10

<a name="h.9tu8znned25x"></a>ligation

<a name="h.7ggfrz5jwixt"></a>insert: i5-26-10 Vector i5-26-5

<a name="h.lr1ucm3je5ch"></a>	<a name="h.1tdfkaxf72hu"></a>3:1	<a name="h.irgy751q45bb"></a>0:1
<a name="h.29frdg7sf7qs"></a>insert	<a name="h.3jo10h4lfbwa"></a>6.3	<a name="h.66r7wdylz39h"></a>0
<a name="h.grelxwnto9yw"></a>vector	<a name="h.3uzirpqv6238"></a>2	<a name="h.f2tp69mzqtu8"></a>2
<a name="h.mr4s0tbd3zsx"></a>buffer	<a name="h.5d0nzx5y3dd7"></a>2	<a name="h.b7g51hyqpul1"></a>2
<a name="h.voe786qcl1rf"></a>ligase	<a name="h.q5ym06cr9hin"></a>1	<a name="h.o7p5fhdl1qx2"></a>1
<a name="h.kvnjo4exssvp"></a>water	<a name="h.xaalwyd7nrzb"></a>8.7	<a name="h.8xb8uwpml5mk"></a>15
<a name="h.rrlxvuele5sw"></a>total	<a name="h.mwokkpi7pthu"></a>20	<a name="h.xke9jmf50d4i"></a>20

<a name="h.mrcdf6e8qi1q"></a> 1 2

<a name="h.8pcb8d35m6as"></a>insert i5-26-10 vector i5-26-4

<a name="h.lr1ucm3je5ch"></a>	<a name="h.1tdfkaxf72hu"></a>3:1	<a name="h.irgy751q45bb"></a>0:1
<a name="h.29frdg7sf7qs"></a>insert	<a name="h.3jo10h4lfbwa"></a>6.6	<a name="h.66r7wdylz39h"></a>0
<a name="h.grelxwnto9yw"></a>vector	<a name="h.3uzirpqv6238"></a>2.5	<a name="h.f2tp69mzqtu8"></a>2.5
<a name="h.mr4s0tbd3zsx"></a>buffer	<a name="h.5d0nzx5y3dd7"></a>2	<a name="h.b7g51hyqpul1"></a>2
<a name="h.voe786qcl1rf"></a>ligase	<a name="h.q5ym06cr9hin"></a>1	<a name="h.o7p5fhdl1qx2"></a>1
<a name="h.kvnjo4exssvp"></a>water	<a name="h.xaalwyd7nrzb"></a>7.3	<a name="h.8xb8uwpml5mk"></a>14.5
<a name="h.rrlxvuele5sw"></a>total	<a name="h.mwokkpi7pthu"></a>20	<a name="h.xke9jmf50d4i"></a>20

<a name="h.2zzkjr266vom"></a> 3 4

<a name="h.c7ej88x5dknt"></a>insert: i5-26-8 vector: i5-26-6

<a name="h.rp0xx4ndzdnc"></a>	<a name="h.mcz4896ozeum"></a>3:1	<a name="h.vxgp54ojxuvj"></a>0:1
<a name="h.qausojphcizg"></a>insert	<a name="h.9nynadi0m07"></a>4.4	<a name="h.tqh3z8gi5eem"></a>0
<a name="h.g8daez7kpm6m"></a>vector	<a name="h.zbn5uiicfri2"></a>2	<a name="h.de9a00gs89ot"></a>2
<a name="h.qc6mrhqcnlyr"></a>buffer	<a name="h.pcgj2fa8t2fk"></a>2	<a name="h.lz8uvgragy7d"></a>2
<a name="h.nxwjrpkmzq6v"></a>ligase	<a name="h.atwez3jpqgqh"></a>1	<a name="h.knj4v3lna52d"></a>1
<a name="h.3mc1x2tvomwu"></a>water	<a name="h.yuz1fyimsmsf"></a>10.6	<a name="h.6bzfezftr4lh"></a>15
<a name="h.eqqsvyoz2beq"></a>total	<a name="h.slsnq3q69jdh"></a>20	<a name="h.za6kz0jioq5y"></a>20

<a name="h.5jjln8h6k8kb"></a> 5 6

<a name="h.y7etqamfqm6a"></a>insert i5-26-9 Vector i5-26-6

<a name="h.o9c2o0qsvdh"></a>	<a name="h.xr45vdntahox"></a>3:1
<a name="h.xg71pdalpo99"></a>insert	<a name="h.9i2exop4xi3i"></a>5.1
<a name="h.tnj7o45aslz6"></a>vector	<a name="h.4jq6y5rz3ega"></a>2
<a name="h.8rl0imxjpnln"></a>buffer	<a name="h.h7ae1fba1ius"></a>2
<a name="h.3n097hgtubt9"></a>ligase	<a name="h.1h6286lbzxyx"></a>1
<a name="h.6j9jobvb4735"></a>water	<a name="h.jdxhm6vm65zt"></a>9.9
<a name="h.qf5w6k7j91ln"></a>total	<a name="h.7srltd35ouat"></a>20

<a name="h.2oy2xb6en0p2"></a> 7

<a name="h.sio52xcr0lwf"></a>8/10

<a name="h.424r9tg71t6"></a>Transformation

<a name="h.qv53featg3ve"></a>1 → i5-30-1 → 5+ colonies [pFru-RBS-GFP]

<a name="h.2mrodfqf4cj5"></a>2 → i5-30-2 → 0 colonies

<a name="h.h1ailh1sd55k"></a>3 → i5-30-3 → 5 colonies f[pScr-RBS-GFP]

<a name="h.h9xnfa7sv8gj"></a>4 → i5-30-4→ 0 colonies

<a name="h.f5rdsf7n4y3l"></a>5 → i5-30-5 → 60+ colonies [CP-Cra]

<a name="h.64iburyxf127"></a>6 → i5-30-6 → 60+ colonies

<a name="h.bi19hrunzili"></a>7 → i5-30-7 → 60+ colonies [CP-ScR]

Revision as of 20:55, 23 September 2013 (view source) Alecspin (Talk \| contribs) (Created page with "<p class="c2"><span>7/28/2013</span></p><p class="c2"><a name="h.1w7xzdr4u63c"></a><span>PCR</span></p><p class="c1"><a name="h.qqxu5pia3myb"></a></p><a href="#" name="0069c024bb...") ← Older edit		Latest revision as of 21:02, 23 September 2013 (view source) Alecspin (Talk \| contribs)
Line 1:		Line 1:
-	<p class="c2"><span>7/28/2013</span></p><p class="c2"><a name="h.1w7xzdr4u63c"></a><span>PCR</span></p><p class="c1"><a name="h.qqxu5pia3myb"></a></p><a href="#" name="0069c024bb369e1d214ad9b0518ab8474de052f6"></a><a href="#" name="9"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c36"><p class="c4 c2"><a name="h.l98dou3iv8o"></a><span>Primers</span></p></td><td class="c36"><p class="c4 c2"><a name="h.iylmgzh0plu0"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.h7cygypzygvd"></a><span>2</span></p></td><td class="c36"><p class="c4 c2"><a name="h.htgvzlr3zeg1"></a><span>3</span></p></td><td class="c36"><p class="c4 c2"><a name="h.c9zuyf785t1k"></a><span>4</span></p></td><td class="c36"><p class="c4 c2"><a name="h.atfwyo73js4d"></a><span>5</span></p></td><td class="c36"><p class="c4 c2"><a name="h.h7dqlwqgya0e"></a><span>6</span></p></td><td class="c36"><p class="c4 c2"><a name="h.6ekecfdp08zm"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.emgt12bv0g4g"></a><span>8</span></p></td><td class="c36"><p class="c4 c2"><a name="h.cmpkt9yv0iat"></a><span>9</span></p></td><td class="c36"><p class="c4 c2"><a name="h.je7t7q6tv1rf"></a><span>10</span></p></td></tr><tr class="c8"><td class="c36"><p class="c4 c2"><a name="h.s8aq2lbk47y3"></a><span>DNA</span></p></td><td class="c36"><p class="c4 c2"><a name="h.rwp11tgj4ahh"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.z2tat31a3gar"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.i98vrbkqsqnm"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.usa99qhtlxql"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.yk37pz4wnolg"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.93mvjlyrb5jn"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.dz2ctq9w2aqk"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.sotzy92rv1s"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.li3a7e2bdvn1"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.t6ryvfn516e3"></a><span>1</span></p></td></tr><tr class="c8"><td class="c36"><p class="c4 c2"><a name="h.e6r7lvtrxib4"></a><span>F</span></p></td><td class="c36"><p class="c4 c2"><a name="h.9c8jtdfzcapw"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.glq9z4l3ebu"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.cy5bp9k13ic0"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.9d7e08bmzv1d"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.gduu3qbma75b"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.z6t16hk7if5r"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.39dyx564wgqh"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.40oje46pms7d"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.np1vcv5u8g5k"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.emqtdc1e10t6"></a><span>1</span></p></td></tr><tr class="c8"><td class="c36"><p class="c4 c2"><a name="h.azwizg70184b"></a><span>R</span></p></td><td class="c36"><p class="c4 c2"><a name="h.o5hmje4g5c8u"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.ukf167shdgjo"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.o41h7oavaiam"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.tf8kg14l1y3r"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.irnniwmsayy0"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.ygpgl6h0jhre"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.i71phhtxr5lq"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.o1o13ry40x9f"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.752xc4awlv9t"></a><span>1</span></p></td><td class="c36"><p class="c4 c2"><a name="h.s82d9wj9hh0f"></a><span>1</span></p></td></tr><tr class="c8"><td class="c36"><p class="c4 c2"><a name="h.wwgb8rb2caqx"></a><span>Phusion</span></p></td><td class="c36"><p class="c4 c2"><a name="h.ot6w07vgsmho"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.j0qp0j8yylz"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.9ka3k0y4zl04"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.tf6hvlrn1u79"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.pnybwmk1amlp"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.vyeifkgxpagy"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.vy8zdbxwo69v"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.13lc0p7rp4g"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.9rvgw4inrhql"></a><span>10</span></p></td><td class="c36"><p class="c4 c2"><a name="h.bgvpr1ym61fz"></a><span>10</span></p></td></tr><tr class="c8"><td class="c36"><p class="c4 c2"><a name="h.zfb5f4i9rdii"></a><span>Water</span></p></td><td class="c36"><p class="c4 c2"><a name="h.sta2t5jqzrvw"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.icutzgtuflsq"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.dvu38r3f8g1t"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.62umehxctlvk"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.1zld32o1mbx4"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.q7dclgac2akm"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.e53dqcg8lwdh"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.thms7q28qo7y"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.5hwyopy6g198"></a><span>7</span></p></td><td class="c36"><p class="c4 c2"><a name="h.h268jsv0xfi7"></a><span>7</span></p></td></tr></tbody></table><p class="c1"><a name="h.ptzum1ut187j"></a></p><p class="c2"><a name="h.625habuoaedf"></a><span>Digestion </span></p><p class="c1"><a name="h.aksdanvknykf"></a></p><a href="#" name="c5f20c3bfc7fb1e9388cd5cdff6e1ead54d8a697"></a><a href="#" name="10"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c51"><p class="c1 c4"><a name="h.537gplby40eo"></a></p></td><td class="c104"><p class="c4 c2"><a name="h.xqc0lppe6krv"></a><span>5 (i5-13-9)</span></p></td><td class="c78"><p class="c4 c2"><a name="h.ubh03cwp1deq"></a><span>6 (i3-13-10)</span></p></td><td class="c76"><p class="c4 c2"><a name="h.o2wbmaardiau"></a><span>7 (i3-13-11)</span></p></td><td class="c33"><p class="c4 c2"><a name="h.ovwu3kvq8nyk"></a><span>8 (i3-13-12)</span></p></td><td class="c96"><p class="c4 c2"><a name="h.y6s4vn5x3iav"></a><span>9 (i3-13-13)</span></p></td><td class="c92"><p class="c4 c2"><a name="h.smdjo587yn7d"></a><span>i5-10-3 (i5-13-14)</span></p></td></tr><tr class="c8"><td class="c51"><p class="c4 c2"><a name="h.xntrywweu8of"></a><span>DNA</span></p></td><td class="c104"><p class="c4 c2"><a name="h.lv3064b3lmj"></a><span>30</span></p></td><td class="c78"><p class="c4 c2"><a name="h.8ph34t10kt1k"></a><span>30</span></p></td><td class="c76"><p class="c4 c2"><a name="h.rsoug65yaof9"></a><span>30</span></p></td><td class="c33"><p class="c4 c2"><a name="h.er67n63cg2v7"></a><span>30</span></p></td><td class="c96"><p class="c4 c2"><a name="h.bqbab170861d"></a><span>30</span></p></td><td class="c92"><p class="c4 c2"><a name="h.igemoh6tuopz"></a><span>30</span></p></td></tr><tr class="c8"><td class="c51"><p class="c4 c2"><a name="h.3z2okkavkzgn"></a><span>NE Buffer</span></p></td><td class="c104"><p class="c4 c2"><a name="h.23pmrd3j3kfz"></a><span>5</span></p></td><td class="c78"><p class="c4 c2"><a name="h.vsrsr7pdqtt0"></a><span>5</span></p></td><td class="c76"><p class="c4 c2"><a name="h.epa3h1153om3"></a><span>5</span></p></td><td class="c33"><p class="c4 c2"><a name="h.4ikn46vluxyw"></a><span>5</span></p></td><td class="c96"><p class="c4 c2"><a name="h.gxe844eltiua"></a><span>5</span></p></td><td class="c92"><p class="c4 c2"><a name="h.iaz8nua1eyfu"></a><span>5</span></p></td></tr><tr class="c8"><td class="c51"><p class="c4 c2"><a name="h.2uuz4e40isx1"></a><span>EcoRI</span></p></td><td class="c104"><p class="c4 c2"><a name="h.i0xp596p5fw1"></a><span>1</span></p></td><td class="c78"><p class="c4 c2"><a name="h.sqlcqmgvj173"></a><span>1</span></p></td><td class="c76"><p class="c4 c2"><a name="h.cq48ztpsv71d"></a><span>1</span></p></td><td class="c33"><p class="c4 c2"><a name="h.ck15m3arqdrd"></a><span>1</span></p></td><td class="c96"><p class="c4 c2"><a name="h.93tkn3hqbi9w"></a><span>1</span></p></td><td class="c92"><p class="c4 c2"><a name="h.w0glvf9ljf32"></a><span>1</span></p></td></tr><tr class="c8"><td class="c51"><p class="c4 c2"><a name="h.77qgyz3qlwhs"></a><span>Prst I</span></p></td><td class="c104"><p class="c4 c2"><a name="h.dotv3dly1w9n"></a><span>1</span></p></td><td class="c78"><p class="c4 c2"><a name="h.m0cauz10idxo"></a><span>1</span></p></td><td class="c76"><p class="c4 c2"><a name="h.pzaip3q8vyky"></a><span>1</span></p></td><td class="c33"><p class="c4 c2"><a name="h.5ccfp79x0sya"></a><span>1</span></p></td><td class="c96"><p class="c4 c2"><a name="h.p1vfow1jids1"></a><span>1</span></p></td><td class="c92"><p class="c4 c2"><a name="h.mq5gkij39x1m"></a><span>1</span></p></td></tr><tr class="c8"><td class="c51"><p class="c4 c2"><a name="h.tofmw9c4te4v"></a><span>Water</span></p></td><td class="c104"><p class="c4 c2"><a name="h.6dwq0bt714dx"></a><span>13</span></p></td><td class="c78"><p class="c4 c2"><a name="h.t6qsxr11k62i"></a><span>13</span></p></td><td class="c76"><p class="c4 c2"><a name="h.dbq1oskgtj8a"></a><span>13</span></p></td><td class="c33"><p class="c4 c2"><a name="h.j6u49lawj21h"></a><span>13</span></p></td><td class="c96"><p class="c4 c2"><a name="h.loge284qwxle"></a><span>13</span></p></td><td class="c92"><p class="c4 c2"><a name="h.vmjjkyo4l4vt"></a><span>13</span></p></td></tr></tbody></table><p class="c2"><a name="h.kvb5pahdagn6"></a><span>7/24/2013</span></p><p class="c2"><a name="h.d8tsh5kkz4h0"></a><span>PCR</span></p><p class="c1"><a name="h.hskqvv6hw324"></a></p><a href="#" name="a83db890a01d761808d0df4a6f54456aee0869c6"></a><a href="#" name="11"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.q47n76xr18ri"></a><span>Primers</span></p></td><td class="c3"><p class="c4 c2"><a name="h.7rkpqmb3uwac"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.8kvhh4ls95g1"></a><span>2</span></p></td><td class="c3"><p class="c4 c2"><a name="h.l6ktdui81p2i"></a><span>3</span></p></td><td class="c3"><p class="c4 c2"><a name="h.wg2w0swmphbf"></a><span>4</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.aupmsss8kqaz"></a><span>DNA</span></p></td><td class="c3"><p class="c4 c2"><a name="h.us5i9ie2ief7"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.d1arncc0oq0i"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.wx2lt1gmgejp"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.moqlznfx8kxv"></a><span>1</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.ea6xpevky1n5"></a><span>R</span></p></td><td class="c3"><p class="c4 c2"><a name="h.2lo40l8nn351"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.c4nvgln2gort"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.l4ns7ijsrf9a"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.v67x0t1gynjr"></a><span>1</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.g728ofbrsyd2"></a><span>F</span></p></td><td class="c3"><p class="c4 c2"><a name="h.log4kdfc2xut"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.2tilhu3yk376"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.mrnv1jd0m6vk"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.19fqyxi4lj5b"></a><span>1</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.wgb3khr5skw2"></a><span>Phusion</span></p></td><td class="c3"><p class="c4 c2"><a name="h.63er7ixlb9ud"></a><span>10</span></p></td><td class="c3"><p class="c4 c2"><a name="h.3mxustbm97sx"></a><span>10</span></p></td><td class="c3"><p class="c4 c2"><a name="h.katv3qd44lkw"></a><span>10</span></p></td><td class="c3"><p class="c4 c2"><a name="h.q7gjuhvs0um"></a><span>10</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.4hdp70vtlra"></a><span>Water</span></p></td><td class="c3"><p class="c4 c2"><a name="h.sndqj4z3vcvl"></a><span>7</span></p></td><td class="c3"><p class="c4 c2"><a name="h.1f065lbzz1z"></a><span>7</span></p></td><td class="c3"><p class="c4 c2"><a name="h.gvon0ul75w87"></a><span>7</span></p></td><td class="c3"><p class="c4 c2"><a name="h.xoffqy49dsnd"></a><span>7</span></p></td></tr></tbody></table><p class="c1"><a name="h.vw3bshxwwuxf"></a></p><p class="c2"><a name="h.koawdqsmnyoh"></a><span>1        i5-14-1                 33.3 ng/ul        (Com C)</span></p><p class="c2"><a name="h.fv1vt78mg7ru"></a><span>2        i5-14-2                47.5 ng/ul        (Com D)</span></p><p class="c2"><a name="h.3x1x0benh4pe"></a><span>3        i5-14-3                38.4 ng/ul        (Com E)</span></p><p class="c2"><a name="h.4z3kd4o6avp1"></a><span>4        i5-14-4                27.8 ng/ul        (Com A)</span></p><p class="c1"><a name="h.l08jj5jvxhhm"></a></p><p class="c2"><a name="h.byev1ekaa63a"></a><span>7/30/2013</span></p><p class="c2"><a name="h.hhq1lr5p0okp"></a><span>Digestion </span></p><p class="c2"><a name="h.rz8lt1ph5jxn"></a><span>1 (i5-14-1)        (Com C)</span></p><p class="c2"><a name="h.n0uh9rz1mf4q"></a><span>DNA                30        (0 left)</span></p><p class="c2"><a name="h.kneof781dyz1"></a><span>NEBuffer        5</span></p><p class="c2"><a name="h.i4qtg9crqsm6"></a><span>EcoRI                1</span></p><p class="c2"><a name="h.db59aiebk0lj"></a><span>PstI                1</span></p><p class="c2"><a name="h.fw9odm6ololp"></a><span>Water                13</span></p><p class="c2"><a name="h.ut30mxfqsmhf"></a><span>→ new i5-14-1</span></p><p class="c1"><a name="h.2flutjvnrfl1"></a></p><p class="c2"><a name="h.qyltrisqlr82"></a><span>Gel extraction i5-14-1</span></p><p class="c1"><a name="h.lag2kt5dnjgh"></a></p><p class="c2"><a name="h.mvk988hr22kv"></a><span>i5-13-9 (Com B)                8.6 ng/ul </span></p><p class="c2"><a name="h.dj3ugc0biey"></a><span>i5-13-10 (ScrR)                12 ng/ul</span></p><p class="c2"><a name="h.5qit7p46px2g"></a><span>i513-11(pScr)                14.4 ng/ul</span></p><p class="c2"><a name="h.3do6oe7lvk2u"></a><span>i5-13-12 (Cra)                15.1 ng/ul</span></p><p class="c2"><a name="h.5y516xtktq34"></a><span>i5-13-13(pFru)                11 ng/ul</span></p><p class="c2"><a name="h.4boqh7b34zdl"></a><span>i5-13-14(Chlora backbone)        24 ng/ul</span></p><p class="c2"><a name="h.s5lp4mbltzhy"></a><span>i5-15-1 (Com C)                13.9 ng/ul</span></p><p class="c1"><a name="h.8r9vz5eadw0t"></a></p><p class="c2"><a name="h.ut6ozfvulmxz"></a><span>Ligation </span></p><p class="c2"><a name="h.pfehlplbqkke"></a><span>Vector i5-13-14 (Chlora)</span></p><p class="c1"><a name="h.8r7mn68yco2x"></a></p><p class="c1"><a name="h.i07o62sb0skt"></a></p><a href="#" name="50d13a574052472cd642da77fe9561faf1a141d9"></a><a href="#" name="12"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c18"><p class="c4 c2"><a name="h.rwclbd7ju5o6"></a><span>Insert:</span></p></td><td class="c18"><p class="c4 c2"><a name="h.eapxxn7uc1g6"></a><span>i5-13-9</span></p></td><td class="c18"><p class="c4 c2"><a name="h.8yh49v9ynpyf"></a><span>i5-13-9</span></p></td><td class="c18"><p class="c4 c2"><a name="h.z9qy3zix2fy9"></a><span>i5-13-10</span></p></td><td class="c18"><p class="c4 c2"><a name="h.pjoqdlvv3lkm"></a><span>i5-13-10</span></p></td><td class="c18"><p class="c4 c2"><a name="h.wq1gnmk3rbzf"></a><span>i5-13-11</span></p></td><td class="c18"><p class="c4 c2"><a name="h.n8s28blj6ch8"></a><span>i5-13-11</span></p></td><td class="c18"><p class="c4 c2"><a name="h.vzyxdfb5t3sd"></a><span>i5-13-12</span></p></td><td class="c18"><p class="c4 c2"><a name="h.2qrdexvawwpk"></a><span>i5-13-12</span></p></td><td class="c18"><p class="c4 c2"><a name="h.z6v5lw64ahad"></a><span>i5-13-13</span></p></td><td class="c18"><p class="c4 c2"><a name="h.y5i9fqvu88i6"></a><span>i5-13-13</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ucy77na90940"></a><span>i5-15-1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.n4drv21h8wzz"></a><span>i5-15-1</span></p></td></tr><tr class="c8"><td class="c18"><p class="c1 c4"><a name="h.ddozs2852nhp"></a></p></td><td class="c18"><p class="c4 c2"><a name="h.4v3arya9n04w"></a><span>3:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.lze3kxcsc4ks"></a><span>0:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.7gpyhvx1ddwn"></a><span>3:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.tb20iaswej"></a><span>0:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.hf9fz1ks5wzs"></a><span>3:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.yvfgmo49urdc"></a><span>0:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.xbpyfe5ghwhd"></a><span>3:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.s365vqt413uu"></a><span>0:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.tj1zd9fyq725"></a><span>3:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.gv8xkigvkbxs"></a><span>0:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.bn78bbm9lzz5"></a><span>3:1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.11iltw2g7gja"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c18"><p class="c4 c2"><a name="h.8xwue3hhxoj2"></a><span>Vector</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ueapcumgr70z"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.a90678jyza68"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.e6e899jjwqh"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.v9uy9dru9lha"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.7s651ehvei4b"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.i107go106z3z"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.xhtik8jfr5t8"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.7jycjrl59rkh"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.bw4ew4c4843j"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.1yf4govsdmmz"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.dy0o6cu8osv8"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.e1bg38dvm6ts"></a><span>2</span></p></td></tr><tr class="c8"><td class="c18"><p class="c4 c2"><a name="h.cykgsr2weglr"></a><span>insert</span></p></td><td class="c18"><p class="c4 c2"><a name="h.eizukidl4cyc"></a><span>7.5</span></p></td><td class="c18"><p class="c4 c2"><a name="h.kog90q6bx275"></a><span>0</span></p></td><td class="c18"><p class="c4 c2"><a name="h.98vnva3vgr2u"></a><span>6</span></p></td><td class="c18"><p class="c4 c2"><a name="h.9u7gxwmfqfpt"></a><span>0</span></p></td><td class="c18"><p class="c4 c2"><a name="h.azeiwoyo9gg3"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.shdnosdzcw16"></a><span>0</span></p></td><td class="c18"><p class="c4 c2"><a name="h.xhdyltdzx6ea"></a><span>5</span></p></td><td class="c18"><p class="c4 c2"><a name="h.qb978w6jidz"></a><span>0</span></p></td><td class="c18"><p class="c4 c2"><a name="h.mdvwwm8w3gr2"></a><span>1.5</span></p></td><td class="c18"><p class="c4 c2"><a name="h.berxsw3ctoj9"></a><span>0</span></p></td><td class="c18"><p class="c4 c2"><a name="h.bws8wghda8eq"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.exx4y5x68751"></a><span>0</span></p></td></tr><tr class="c8"><td class="c18"><p class="c4 c2"><a name="h.ufw4a7mv08qf"></a><span>buffer</span></p></td><td class="c18"><p class="c4 c2"><a name="h.e82ys14lie5g"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.w3awm2jc8x62"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ip79buro7z35"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.2jwvv8pad0y8"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.eui2glumbma9"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ij6a4wggvhr8"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.5erup0qxiaoa"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.34drqu9lsiip"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.sxt14q53zna"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.zeib64cg79d2"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.w76ejfa0vgam"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.sli6mh394kb5"></a><span>2</span></p></td></tr><tr class="c8"><td class="c18"><p class="c4 c2"><a name="h.v35ueq5cr2ki"></a><span>ligase</span></p></td><td class="c18"><p class="c4 c2"><a name="h.jp2k3gq27m9p"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.l2ezm01epi51"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ep4rxobcrnm0"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.gr1lj3ltfo95"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.gf8vy915vn"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.trcwr9gqzc95"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ap4irlbntl3b"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.brtf4pa8s3tg"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.56a01qf31wqd"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.8nysqqn9xpga"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.cgwytf5eesu9"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.b39j5p6tch1k"></a><span>1</span></p></td></tr><tr class="c8"><td class="c18"><p class="c4 c2"><a name="h.kf7kmjciwkpj"></a><span>water</span></p></td><td class="c18"><p class="c4 c2"><a name="h.l5mxf8eibg1"></a><span>7.5</span></p></td><td class="c18"><p class="c4 c2"><a name="h.4hvglti0oxpi"></a><span>15</span></p></td><td class="c18"><p class="c4 c2"><a name="h.jr1bst19a9wz"></a><span>9</span></p></td><td class="c18"><p class="c4 c2"><a name="h.wzkvjibfhcnb"></a><span>15</span></p></td><td class="c18"><p class="c4 c2"><a name="h.pndyfze6qn5i"></a><span>14</span></p></td><td class="c18"><p class="c4 c2"><a name="h.gub9i3ohcehi"></a><span>15</span></p></td><td class="c18"><p class="c4 c2"><a name="h.i2kexdv8pq33"></a><span>10</span></p></td><td class="c18"><p class="c4 c2"><a name="h.o7nzrkza94cd"></a><span>15</span></p></td><td class="c18"><p class="c4 c2"><a name="h.xpe8r41l59s0"></a><span>13.5</span></p></td><td class="c18"><p class="c4 c2"><a name="h.55gekl6q1m72"></a><span>15</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ym8dfmbzpnfk"></a><span>14</span></p></td><td class="c18"><p class="c4 c2"><a name="h.1nuuijugz687"></a><span>15</span></p></td></tr><tr class="c8"><td class="c18"><p class="c4 c2"><a name="h.xt2svhv3b8l4"></a><span>total</span></p></td><td class="c18"><p class="c4 c2"><a name="h.2tn6w1uqmqxz"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.sdpcuzjyl7ts"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ourvjjokdadc"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.bsdbvy1o7wln"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.jwaa3xuxas9"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.hqmcbdgpw0lx"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.e0ehwrwd4agz"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.zfiuqf321edu"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ps0ewmqtqv9o"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.n6pyhtm59dbi"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.y4lt0enlcu3u"></a><span>20</span></p></td><td class="c18"><p class="c4 c2"><a name="h.riegvhuvgl4n"></a><span>20</span></p></td></tr><tr class="c8"><td class="c18"><p class="c1 c4"><a name="h.abfr1wtzld8l"></a></p></td><td class="c18"><p class="c4 c2"><a name="h.eue2n4kohh0l"></a><span>1</span></p></td><td class="c18"><p class="c4 c2"><a name="h.ef50nw1y7qha"></a><span>2</span></p></td><td class="c18"><p class="c4 c2"><a name="h.2l6f7excv42h"></a><span>3</span></p></td><td class="c18"><p class="c4 c2"><a name="h.3c6fmyyunrm7"></a><span>4</span></p></td><td class="c18"><p class="c4 c2"><a name="h.l5ht1kgx2r3d"></a><span>5</span></p></td><td class="c18"><p class="c4 c2"><a name="h.zi9axt96eqt2"></a><span>6</span></p></td><td class="c18"><p class="c4 c2"><a name="h.qr6e4yv2ily7"></a><span>7</span></p></td><td class="c18"><p class="c4 c2"><a name="h.exxb2fxp0zbw"></a><span>8</span></p></td><td class="c18"><p class="c4 c2"><a name="h.kuz4x1yluh03"></a><span>9</span></p></td><td class="c18"><p class="c4 c2"><a name="h.7luzyy8w7tqz"></a><span>10</span></p></td><td class="c18"><p class="c4 c2"><a name="h.wzwxx8s2jhfe"></a><span>11</span></p></td><td class="c18"><p class="c4 c2"><a name="h.c4dyb9ecvegy"></a><span>12</span></p></td></tr></tbody></table><p class="c1"><a name="h.gfculfarh7ou"></a></p><p class="c2"><a name="h.mhtosyfxiplo"></a><span>Transformation </span></p><p class="c2"><a name="h.i3wtjhdwzin3"></a><span>1</span></p><p class="c2"><a name="h.yqiuna5qzuv"></a><span>2</span></p><p class="c2"><a name="h.6506ohlh2qwn"></a><span>3</span></p><p class="c2"><a name="h.ic0zbvoiwg54"></a><span>4</span></p><p class="c2"><a name="h.7jw4qtpuq8u"></a><span>5</span></p><p class="c2"><a name="h.vxoc92u740ec"></a><span>6</span></p><p class="c2"><a name="h.xw9bu3ftnlrz"></a><span>7</span></p><p class="c2"><a name="h.wz32he1cowmw"></a><span>8</span></p><p class="c2"><a name="h.xu12aug1fjsk"></a><span>9</span></p><p class="c2"><a name="h.6nxy36gth9ap"></a><span>10</span></p><p class="c2"><a name="h.6vj90p5kdqyh"></a><span>11</span></p><p class="c2"><a name="h.2ivfpv951rq5"></a><span>12</span></p><p class="c2"><a name="h.xrfzapxgozv0"></a><span>+ control → i5-10-4</span></p><p class="c1"><a name="h.a30bxd2gr253"></a></p><p class="c2"><a name="h.2sye30drygjv"></a><span>Inconsistent growth (repeat)</span></p><p class="c1"><a name="h.rcwo2mwhmsj8"></a></p><p class="c2"><a name="h.qdwpmlar9fxp"></a><span>7/30</span></p><p class="c2"><a name="h.h2yrqpht6uek"></a><span>Transformation</span></p><p class="c2"><a name="h.g16bsupb0if7"></a><span>1</span></p><p class="c2"><a name="h.yepbrmq46j8j"></a><span>2</span></p><p class="c2"><a name="h.yuzf97e1ycl1"></a><span>3</span></p><p class="c2"><a name="h.4k2gx4fcrj7c"></a><span>4</span></p><p class="c2"><a name="h.ytz71xaxz9i"></a><span>5</span></p><p class="c2"><a name="h.y9wrgq1sinlr"></a><span>6</span></p><p class="c2"><a name="h.f4nyxt67c1g6"></a><span>7</span></p><p class="c2"><a name="h.chgf8ey2p72w"></a><span>8</span></p><p class="c2"><a name="h.6yor6rdyrcfh"></a><span>9</span></p><p class="c2"><a name="h.ysyhpy20dxld"></a><span>10</span></p><p class="c2"><a name="h.wgqbqis3fu9k"></a><span>11</span></p><p class="c2"><a name="h.g2va1il9rme3"></a><span>12</span></p><p class="c2"><a name="h.va67wmeyjr7z"></a><span>+ control → i5-10-4</span></p><p class="c1"><a name="h.l3gyqob9ad8r"></a></p><p class="c2"><a name="h.lyf956klvtnq"></a><span>Inoculate</span></p><p class="c2"><a name="h.cu4kdzhfgn9j"></a><span>From glycerol stock i5-11-5 (pLac-GFP)</span></p><p class="c2"><a name="h.cvxy2odcnx2"></a><span>100.4 ng/ul</span></p><p class="c2"><a name="h.72910tlji9sm"></a><span>96.4 ng/ul        [pLac-GFP]</span></p><p class="c2"><a name="h.gxyw4fotd64k"></a><span>84.3 ng/ul</span></p><p class="c1"><a name="h.blxr8wovn17y"></a></p><p class="c2"><a name="h.1sjg3n4ze7nq"></a><span>7/31</span></p><p class="c2"><a name="h.iwchhdbervw9"></a><span>Transformation Results</span></p><p class="c2"><a name="h.5atic82knicq"></a><span>1 [Com B] → 8 colonies → Picked 3 colonies→ i5-16-1</span></p><p class="c2"><a name="h.k4hslldhwd8r"></a><span>                                                i5-16-2</span></p><p class="c2"><a name="h.16gg4u89efie"></a><span>                                                i5-16-3</span></p><p class="c2"><a name="h.9m4le8otw8qu"></a><span>2→ 50 colonies</span></p><p class="c2"><a name="h.tbck2neavtxc"></a><span>3 [Scr] → 11 colonies → Picked 3 colonies → i5-16-4</span></p><p class="c2"><a name="h.orwfvyt5ue2v"></a><span>                                        i5-16-5</span></p><p class="c2"><a name="h.242xprdzg0b5"></a><span>                                        i5-16-6</span></p><p class="c2"><a name="h.8faqzlhxo0iq"></a><span>4 → 20 colonies</span></p><p class="c2"><a name="h.ds7rd5ef53tw"></a><span>5 [pScr] → 25 colonies → picked 3 colonies → i5-16-7</span></p><p class="c2"><a name="h.uninw76k2wf"></a><span>                                                i5-16-8</span></p><p class="c2"><a name="h.qpdj9aujpd2t"></a><span>                                                i5-16-9</span></p><p class="c2"><a name="h.405vih9kuvu"></a><span>6 → 0 colonies</span></p><p class="c2"><a name="h.ox6mwhqz0f0f"></a><span>7 [Cra] → 50+ colonies → picked 3 colonies → i5-16-10</span></p><p class="c2"><a name="h.3pqoz5468f0a"></a><span>                                                i5-16-11</span></p><p class="c2"><a name="h.g56vdta42xvu"></a><span>                                                i5-16-12</span></p><p class="c2"><a name="h.uf67ey9w9f40"></a><span>8 → 75+ colonies</span></p><p class="c2"><a name="h.aoggldrolu7c"></a><span>9 [pFru] → 75+ colonies → picked 3 colonies → i5-16-13</span></p><p class="c2"><a name="h.z6au5v2jb975"></a><span>                                                i5-16-14</span></p><p class="c2"><a name="h.90uwnto7k70s"></a><span>                                                i5-16-15</span></p><p class="c2"><a name="h.n89z936984bi"></a><span>10 → 11 colonies</span></p><p class="c2"><a name="h.283avhppju1z"></a><span>11 [Com C] → 30 colonies → picked 3 colonies→  i5-16-16</span></p><p class="c2"><a name="h.92qdy4j90bwo"></a><span>                                                i5-16-17</span></p><p class="c2"><a name="h.wsv606ce7ery"></a><span>                                                i5-16-18</span></p><p class="c2"><a name="h.ubmjrqntcnja"></a><span>12→ 30 colonies</span></p><p class="c2"><a name="h.mj4433clon1w"></a><span>13→ 7 colonies</span></p><p class="c2"><a name="h.u0ea7xjc6274"></a><span>14 →2 colonies</span></p><p class="c2"><a name="h.d9vx3nyuimz7"></a><span>15 → 20 colonies</span></p><p class="c2"><a name="h.f2cq7ihd00rt"></a><span>16→ 10 colonies</span></p><p class="c2"><a name="h.9cfq1743wgqc"></a><span>17 → 0 colonies </span></p><p class="c2"><a name="h.okzp12kpgfhd"></a><span>18 → 5 colonies</span></p><p class="c2"><a name="h.m6j8vq7xdy0l"></a><span>+ control → ∞ colonies</span></p><p class="c1"><a name="h.1hxya8nns9er"></a></p><p class="c2"><a name="h.hkvp1ly7bxwx"></a><span>8/1</span></p><p class="c2"><a name="h.gdt42dr1sitx"></a><span>Miniprep</span></p><p class="c2"><a name="h.k16mhl32quoi"></a><span>i5-16-1 [Com B]                7.4</span></p><p class="c2"><a name="h.ykgo7uctfizd"></a><span>i5-16-2 [Com B]                5.4</span></p><p class="c2"><a name="h.b5syoehwe5sd"></a><span>i5-16-3[Com B]                13.7</span></p><p class="c2"><a name="h.mfxia9twmnj"></a><span>i5-16-4 [Scr]                no growth </span></p><p class="c2"><a name="h.601z1zffypq"></a><span>i5-16-5 [Scr]                10.7</span></p><p class="c2"><a name="h.ycdqtoz6j1jh"></a><span>i5-16-6[Scr]                7.8</span></p><p class="c2"><a name="h.ajykp6mwqppf"></a><span>i5-16-7[pScr]                8.4</span></p><p class="c2"><a name="h.ne55wp3gkau3"></a><span>i5-16-8[pScr]                9.1</span></p><p class="c2"><a name="h.e13d54dyzft7"></a><span>i5-16-9[pScr]                10.6</span></p><p class="c2"><a name="h.txo346njagbb"></a><span>i5-16-10 [Cra]                19.9</span></p><p class="c2"><a name="h.c3473p5uadpe"></a><span>i5-16-11[Cra]                5.2</span></p><p class="c2"><a name="h.wbmb3g1s7ugf"></a><span>i5-16-12 [Cra]                6.4</span></p><p class="c2"><a name="h.kv0tfbo2jv8i"></a><span>i5-16-13[pFru]                15.3</span></p><p class="c2"><a name="h.j3t9j0u7xkkq"></a><span>i5-16-14[pFru]                5.4</span></p><p class="c2"><a name="h.ilxeqahhep0j"></a><span>i5-16-15[pFru]                5.3</span></p><p class="c2"><a name="h.iwp9ty6hlxs1"></a><span>i5-16-16[Com C]        2.4</span></p><p class="c2"><a name="h.n4f67hryzt17"></a><span>i5-16-17[Com C]        7.7</span></p><p class="c2"><a name="h.l8oo77q6fsr"></a><span>i5-16-18[Com C]        5.8</span></p><p class="c1"><a name="h.df02fsoc2gkk"></a></p><p class="c2"><a name="h.s0shikkagflk"></a><span>Concentrations too low</span></p><p class="c2"><a name="h.h0j00xjiuue9"></a><span>Redo digestion and ligation (Plan B)</span></p><p class="c1"><a name="h.7k5mc2xcbp6i"></a></p><p class="c2"><a name="h.1h2u7hs3ix23"></a><span>Digestion </span></p><p class="c1"><a name="h.g3z76xurwd39"></a></p><a href="#" name="fdd2c2cc1a30f21ef2d5a146b718253990fe43a8"></a><a href="#" name="13"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c67"><p class="c1 c4"><a name="h.nw7x4tp0kj95"></a></p></td><td class="c86"><p class="c4 c2"><a name="h.b77riogep97k"></a><span>i5-11-5 (100.9 ng/ul) [pLac-RBS-GFP]</span></p></td></tr><tr class="c8"><td class="c67"><p class="c4 c2"><a name="h.7x23o2q63rld"></a><span>DNA</span></p></td><td class="c86"><p class="c4 c2"><a name="h.xs95dkb0qv3g"></a><span>20</span></p></td></tr><tr class="c8"><td class="c67"><p class="c4 c2"><a name="h.qmbaclu4and5"></a><span>NEBuffer</span></p></td><td class="c86"><p class="c4 c2"><a name="h.i4e84vgkrcxx"></a><span>5</span></p></td></tr><tr class="c8"><td class="c67"><p class="c4 c2"><a name="h.9fr4omfw11ah"></a><span>EcoR</span></p></td><td class="c86"><p class="c4 c2"><a name="h.xp4lm573njg7"></a><span>1</span></p></td></tr><tr class="c8"><td class="c67"><p class="c4 c2"><a name="h.fnra7wygpvwz"></a><span>PstI</span></p></td><td class="c86"><p class="c4 c2"><a name="h.k3078dha6squ"></a><span>1</span></p></td></tr><tr class="c8"><td class="c67"><p class="c4 c2"><a name="h.uzdcyicnhuw5"></a><span>Water</span></p></td><td class="c86"><p class="c4 c2"><a name="h.peiv6cad45bh"></a><span>23</span></p></td></tr><tr class="c8"><td class="c67"><p class="c4 c2"><a name="h.wla559qqazui"></a><span>Total</span></p></td><td class="c86"><p class="c4 c2"><a name="h.snx6esd4tjh1"></a><span>50 → i5-17-1</span></p></td></tr></tbody></table><p class="c1"><a name="h.hxsbfqaq1flk"></a></p><p class="c2"><a name="h.ak8khbywm050"></a><span>Digestion</span></p><p class="c1"><a name="h.qpk8acfchyb7"></a></p><a href="#" name="7150480a806c7acfe65074c44a5a56b72df44278"></a><a href="#" name="14"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c42"><p class="c1 c4"><a name="h.plaqos35rnbu"></a></p></td><td class="c42"><p class="c4 c2"><a name="h.oduap9peuhwz"></a><span>i4-45-3</span></p></td><td class="c42"><p class="c4 c2"><a name="h.28slfgkpyrku"></a><span>i4-45-2</span></p></td><td class="c42"><p class="c4 c2"><a name="h.qm7ywscmvd1o"></a><span>i5-11-5 [pLac-RBS-GFP] </span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.2y6nakdykx4n"></a><span>DNA</span></p></td><td class="c42"><p class="c4 c2"><a name="h.nluimw5ad5ii"></a><span>20</span></p></td><td class="c42"><p class="c4 c2"><a name="h.euarp7r4qqr4"></a><span>20</span></p></td><td class="c42"><p class="c4 c2"><a name="h.u7pjsz33yp8u"></a><span>20</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.fnxqh2ibdalu"></a><span>NEBuffer</span></p></td><td class="c42"><p class="c4 c2"><a name="h.kme0h754mt8u"></a><span>5</span></p></td><td class="c42"><p class="c4 c2"><a name="h.pychv9g0t5aj"></a><span>5</span></p></td><td class="c42"><p class="c4 c2"><a name="h.90qha3jcqgai"></a><span>5</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.xp8k4bpokxpe"></a><span>EcoR</span></p></td><td class="c42"><p class="c4 c2"><a name="h.simmuu8727lv"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.d4s682l63gvk"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.g0zvv2bkjcot"></a><span>1</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.lctki6qvqypt"></a><span>PstI</span></p></td><td class="c42"><p class="c4 c2"><a name="h.mvrdl2k9tfm"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.e1qgxndsr3jw"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.pcbwtt3jlera"></a><span>1</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.kn30n1rxa7pm"></a><span>Water</span></p></td><td class="c42"><p class="c4 c2"><a name="h.hfipwz68ut8i"></a><span>23</span></p></td><td class="c42"><p class="c4 c2"><a name="h.9a1getpgeol4"></a><span>23</span></p></td><td class="c42"><p class="c4 c2"><a name="h.xfxc3bw7xdpb"></a><span>23</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.7ubx7vr86r15"></a><span>Total</span></p></td><td class="c42"><p class="c4 c2"><a name="h.2fqkbpkycpxr"></a><span>50</span></p></td><td class="c42"><p class="c4 c2"><a name="h.cd74i22n9ixv"></a><span>50</span></p></td><td class="c42"><p class="c4 c2"><a name="h.2thgvsmqsnes"></a><span>50</span></p></td></tr></tbody></table><p class="c1"><a name="h.rnkzjpj51cq2"></a></p><p class="c2"><a name="h.462qh4ufcpyq"></a><span>Nanodrop i4-45-2        18.4</span></p><p class="c2"><a name="h.qymzp02bzgjz"></a><span>        i4-45-3                28.8</span></p><p class="c1"><a name="h.58tertazj0wl"></a></p><p class="c2"><a name="h.8yjdiim94mo2"></a><span>Ligation</span></p><p class="c2"><a name="h.odd5alle0l7u"></a><span>Mastermix: Vector: 16         Buffer: 16</span></p><p class="c1"><a name="h.9ljnk7muefc"></a></p><p class="c2"><a name="h.750mg81plwxp"></a><span>Vector: i4-45-3</span></p><p class="c1"><a name="h.qky6dflkxf5u"></a></p><a href="#" name="d7efde7569928a4e7ee8fc236d3137185b88940d"></a><a href="#" name="15"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.ko16dp6g3zlu"></a><span>Insert</span></p></td><td class="c35"><p class="c4 c2"><a name="h.g9oyoz6rhqne"></a><span>i5-13-9</span></p></td><td class="c35"><p class="c4 c2"><a name="h.ko16dp6g3zlu"></a><span>i5-13-9</span></p></td><td class="c35"><p class="c4 c2"><a name="h.ko16dp6g3zlu"></a><span>i5-13-10</span></p></td><td class="c35"><p class="c4 c2"><a name="h.g9oyoz6rhqne"></a><span>i5-13-11</span></p></td><td class="c35"><p class="c4 c2"><a name="h.hgf7vl6itpa4"></a><span>i5-13-12</span></p></td><td class="c35"><p class="c4 c2"><a name="h.ke9rrrsnsyde"></a><span>i5-13-13</span></p></td><td class="c35"><p class="c4 c2"><a name="h.1w7eymqeeklq"></a><span>i5-15-1</span></p></td></tr><tr class="c8"><td class="c35"><p class="c1 c4"><a name="h.ldquz8vbyhe6"></a></p></td><td class="c35"><p class="c4 c2"><a name="h.htbyr1ll042h"></a><span>3:1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.qlmj2ypxhaup"></a><span>0:1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.xb0vn1n1rtbt"></a><span>3:1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.ws93oogubgzu"></a><span>3:1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.n5puy2qf1f8"></a><span>3:1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.oikpvcs0iovu"></a><span>3:1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.1hj1ujr3oyqe"></a><span>3:1</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.gnq4j8mok285"></a><span>Insert</span></p></td><td class="c35"><p class="c4 c2"><a name="h.p7qkdz74u435"></a><span>7.5</span></p></td><td class="c35"><p class="c4 c2"><a name="h.do5uf7x3xfce"></a><span>0</span></p></td><td class="c35"><p class="c4 c2"><a name="h.gjadgexwrr8k"></a><span>6</span></p></td><td class="c35"><p class="c4 c2"><a name="h.qv4hhw4bo7xq"></a><span>1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.dj2b0xeslc0r"></a><span>5</span></p></td><td class="c35"><p class="c4 c2"><a name="h.wxppe7l5ijoc"></a><span>1.5</span></p></td><td class="c35"><p class="c4 c2"><a name="h.4rr06z9x2bi2"></a><span>1</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.6nrozzpemgsl"></a><span>Vector</span></p></td><td class="c35"><p class="c4 c2"><a name="h.294k89lar7ku"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.t20x9jisxas4"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.ihz5jvqdk19p"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.mlguz32wkiza"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.thhdugjt5v1a"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.6iohv0przw1"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.t36qi49njmay"></a><span>2</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.ix5ibc2nn2bl"></a><span>Buffer</span></p></td><td class="c35"><p class="c4 c2"><a name="h.rbbw0rx8m512"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.bg1sshpu7d3p"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.dx7eibvymd2p"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.ssu9fv79la3s"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.nmchd34dvlrd"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.o91ingua0t1m"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.67y7n43zvoxa"></a><span>2</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.l9lmdir3u5kt"></a><span>Ligase</span></p></td><td class="c35"><p class="c4 c2"><a name="h.y303ooloe8eo"></a><span>1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.f7r14f39zeo9"></a><span>1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.3guxuh1ts81v"></a><span>1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.fco4l7y7m9i0"></a><span>1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.102mclq61u6e"></a><span>1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.wlk08oxitdt"></a><span>1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.f76a5efgbwy0"></a><span>1</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.hkl9t5ohw35f"></a><span>water</span></p></td><td class="c35"><p class="c4 c2"><a name="h.unws8s6zidez"></a><span>7.5</span></p></td><td class="c35"><p class="c4 c2"><a name="h.lot17dn25adf"></a><span>15</span></p></td><td class="c35"><p class="c4 c2"><a name="h.pjp8seiw7ctd"></a><span>9</span></p></td><td class="c35"><p class="c4 c2"><a name="h.2njbhf4869si"></a><span>14</span></p></td><td class="c35"><p class="c4 c2"><a name="h.azmz7p4j1n6j"></a><span>10</span></p></td><td class="c35"><p class="c4 c2"><a name="h.i79hrut5w68k"></a><span>13.5</span></p></td><td class="c35"><p class="c4 c2"><a name="h.kuwi6l8k42ow"></a><span>14</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.j2utgt1mtyc9"></a><span>Total</span></p></td><td class="c35"><p class="c4 c2"><a name="h.w0wf66iqe0tz"></a><span>20</span></p></td><td class="c35"><p class="c4 c2"><a name="h.7zdyt1ohkrtq"></a><span>20</span></p></td><td class="c35"><p class="c4 c2"><a name="h.l8d72odxorcw"></a><span>20</span></p></td><td class="c35"><p class="c4 c2"><a name="h.i3y4oas8rm7p"></a><span>20</span></p></td><td class="c35"><p class="c4 c2"><a name="h.jrhfboomo7ic"></a><span>20</span></p></td><td class="c35"><p class="c4 c2"><a name="h.7tkqll8rjzny"></a><span>20</span></p></td><td class="c35"><p class="c4 c2"><a name="h.7vhzegboowg7"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.u3kbvtk7cxk"></a><span>1                2                3                4                5                6                7</span></p><p class="c1"><a name="h.mt6betv3gsga"></a></p><p class="c2"><a name="h.vdlf9hfxs3za"></a><span>8/1</span></p><p class="c2"><a name="h.3kl77r7ego4"></a><span>Transformation</span></p><p class="c2"><a name="h.qnxw5fcp5bdl"></a><span>+ control → i5-10-4</span></p><p class="c1"><a name="h.rxixlc9jyz7j"></a></p><p class="c2"><a name="h.d3hn90r6uogh"></a><span>1 → 10 colonies → picked 2 → i5-19-1</span></p><p class="c2"><a name="h.tky8lnz5box"></a><span>                                i5-19-2</span></p><p class="c2"><a name="h.v9a0276oa2c3"></a><span>2 →0 colonies</span></p><p class="c2"><a name="h.8rg3vllobj6l"></a><span>3 →0 colonies</span></p><p class="c2"><a name="h.terx6m36at4p"></a><span>4 →20 colonies → picked 2 → i5-19-3</span></p><p class="c2"><a name="h.ax82ddgvej52"></a><span>                                i5-19-4</span></p><p class="c2"><a name="h.kl8f3p7w9a94"></a><span>5 →50 + colonies → picked 2 → i5-19-5</span></p><p class="c2"><a name="h.rly6ll3758vh"></a><span>                                i5-19-6</span></p><p class="c2"><a name="h.t6yhc2bwlkmr"></a><span>6 →0 colonies (ruined plate)</span></p><p class="c2"><a name="h.txw92pj0umww"></a><span>7 →25  colonies → picked 2 → i5-19-7</span></p><p class="c2"><a name="h.2pg34ohcmptq"></a><span>                                i5-19-8</span></p><p class="c1"><a name="h.t1r4u7513way"></a></p><p class="c2"><a name="h.1yh2ap6r5dkj"></a><span>8/2</span></p><p class="c2"><a name="h.cbb9vyy27u50"></a><span>Transformation</span></p><p class="c2"><a name="h.tkzkibvuxed7"></a><span>+ control → i5-10-4</span></p><p class="c2"><a name="h.ygzhfj4d971e"></a><span>1 → 6 colonies → picked 2 → i5-19-9</span></p><p class="c2"><a name="h.jkleuycfz7gw"></a><span>                                i5-19-10</span></p><p class="c2"><a name="h.92goxt1940i6"></a><span>3 → 20 colonies → picked 2 → i5-19-11</span></p><p class="c2"><a name="h.n4keacizzoq7"></a><span>                                i5-19-12</span></p><p class="c2"><a name="h.1657iecoccwd"></a><span>6→ 8 colonies → picked 2 → i5-19-13</span></p><p class="c2"><a name="h.o4r84ljldi8j"></a><span>                                i5-19-14</span></p><p class="c1"><a name="h.i28qtsm3t7e"></a></p><p class="c2"><a name="h.hupfbsd3ivci"></a><span>8/3 </span></p><p class="c2"><a name="h.h7xzg2fhesfu"></a><span>Miniprep </span></p><p class="c1"><a name="h.785x68lq4t23"></a></p><a href="#" name="5da75b8ad68cb98f581dda94edaf46683d1aed71"></a><a href="#" name="16"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c5"><p class="c1 c4"><a name="h.5324zw47di0h"></a></p></td><td class="c48"><p class="c4 c2"><a name="h.i1tf65pvidmt"></a><span>Concentrations ng/ul</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.v7qvzht8rovt"></a><span>i5-19-1 [Com B]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.oycn5t5zwqw"></a><span>90.3</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.yg0vhvy9xbin"></a><span>i5-19-2 [Com B]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.6y6ofdr2i8h0"></a><span>77.5</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.ykmyu8l6hcn2"></a><span>i5-19-3 [pScr]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.3mr1227qxaog"></a><span>39.6</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.psdo7yxrgz7u"></a><span>i5-19-4 [pScr]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.d2gmy5rmmpa1"></a><span>89.5</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.whyjlta6ggiw"></a><span>i5-19-5 [Cra]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.nvrasbi1dfp7"></a><span>71.6</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.2adme0o5ap57"></a><span>i5-19-6 [Cra]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.jfs9zfjou67u"></a><span>120.2</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.g3ggqz1i48at"></a><span>i5-19-7 [Com C]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.zieop85xawgl"></a><span>124.8</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.al7jvioy9ey8"></a><span>i5-19-8 [Com C]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.qquo1hy4hm57"></a><span>101.6</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.sqlxlp2zkwfk"></a><span>i5-19-9</span></p></td><td class="c48"><p class="c4 c2"><a name="h.v2yehdjyl87j"></a><span>108.9</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.o2jm3ibgeqzn"></a><span>i5-19-10</span></p></td><td class="c48"><p class="c4 c2"><a name="h.r9qkczavx1zz"></a><span>102.3</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.3sd8ogw68ews"></a><span>i5-19-11 [Scr]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.t04wha5b9m3c"></a><span>85.8</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.toa1hyt6erb"></a><span>i5-19-12</span></p></td><td class="c48"><p class="c4 c2"><a name="h.l2uogxg89tml"></a><span>86.9</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.xyabvc51wqmz"></a><span>i5-19-13 [pFru]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.t6elcnx8txdu"></a><span>101.4</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.uwy9t1l2s2f1"></a><span>i5-19-14</span></p></td><td class="c48"><p class="c4 c2"><a name="h.zgdrxg3hnv3k"></a><span>97.8</span></p></td></tr></tbody></table><p class="c1"><a name="h.cb1zi8x4xf3h"></a></p><p class="c1"><a name="h.podivfsbn9ht"></a></p><a href="#" name="e792f2144a5f6b55832d2e140860bcc0027fa187"></a><a href="#" name="17"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c27"><p class="c4 c2"><a name="h.oiv53yapn8kh"></a><span>Digestion</span></p></td><td class="c27"><p class="c4 c2"><a name="h.ou4hb7h0seu9"></a><span>i5-19-1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.qte7npmt8r5c"></a><span>i5-19-2</span></p></td><td class="c27"><p class="c4 c2"><a name="h.t9s41xvjzho"></a><span>i5-19-3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.40i1l3i78is1"></a><span>i5-19-4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.br6zg9exyqka"></a><span>i5-19-5</span></p></td><td class="c27"><p class="c4 c2"><a name="h.ii3zqc2ors8j"></a><span>i5-19-6</span></p></td><td class="c27"><p class="c4 c2"><a name="h.fnvbqzaz7j1v"></a><span>i5-19-7</span></p></td><td class="c27"><p class="c4 c2"><a name="h.foy8blyca3l"></a><span>i5-19-8</span></p></td></tr><tr class="c8"><td class="c27"><p class="c4 c2"><a name="h.rf3ds9ps6nzd"></a><span>DNA</span></p></td><td class="c27"><p class="c4 c2"><a name="h.zfo8ozunugrr"></a><span>4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.68oznqb11yif"></a><span>4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.1cme7yxrlqj6"></a><span>4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.5l2uwmewg7ig"></a><span>4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.58m87rj49d9u"></a><span>4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.kvb2741eni0g"></a><span>4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.lsdtxms32y8a"></a><span>4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.c1b212wrflbg"></a><span>4</span></p></td></tr><tr class="c8"><td class="c27"><p class="c4 c2"><a name="h.xikbro4ycyy1"></a><span>Buffer</span></p></td><td class="c27"><p class="c4 c2"><a name="h.vqsvtvp4raop"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.jqxro38sinut"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.2wn1xtn04gdc"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.esnrwhx0uqms"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.ug391abaxoi8"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.4n9bhe83qr01"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.v2f5x9dp0y5s"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.905i5wpg00mj"></a><span>1</span></p></td></tr><tr class="c8"><td class="c27"><p class="c4 c2"><a name="h.ihwa2erhrsb"></a><span>EcoR</span></p></td><td class="c27"><p class="c4 c2"><a name="h.41npynb4vjfh"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.guc33o4xo1ny"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.70bbc5qgkzwy"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.m2ea79t163dd"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.o4d1umcs55jw"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.rl19xxze3i3y"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.nh58fgbq8r36"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.mi69vlcxfy8l"></a><span>1</span></p></td></tr><tr class="c8"><td class="c27"><p class="c4 c2"><a name="h.mwpm9bcdyuz"></a><span>PstI</span></p></td><td class="c27"><p class="c4 c2"><a name="h.pbvtqamppw6t"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.gbl8y6odfyfe"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.8adi8qp99j8w"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.260azt8lkiok"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.41nqramq4vdp"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.gad2myviufeq"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.8cusr7ukuysz"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.kiiioy1rzgw8"></a><span>1</span></p></td></tr><tr class="c8"><td class="c27"><p class="c4 c2"><a name="h.41vrfxo8n7q1"></a><span>Water</span></p></td><td class="c27"><p class="c4 c2"><a name="h.dk5jox3vckg2"></a><span>3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.a2vkupkprp96"></a><span>3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.5dsl3xrxgwhv"></a><span>3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.c860uhtzb5c"></a><span>3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.iyx6d43bxlro"></a><span>3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.meo2phn740du"></a><span>3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.x8ehq55mafpt"></a><span>3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.nw7dgbn47q03"></a><span>3</span></p></td></tr><tr class="c8"><td class="c27"><p class="c4 c2"><a name="h.k9qik7fcg9bf"></a><span>Total</span></p></td><td class="c27"><p class="c4 c2"><a name="h.cul31bek5537"></a><span>10</span></p></td><td class="c27"><p class="c4 c2"><a name="h.f1xx1r7dzjjr"></a><span>10</span></p></td><td class="c27"><p class="c4 c2"><a name="h.ba6wq53qokt0"></a><span>10</span></p></td><td class="c27"><p class="c4 c2"><a name="h.41opoprgaair"></a><span>10</span></p></td><td class="c27"><p class="c4 c2"><a name="h.1ncbe75oart8"></a><span>10</span></p></td><td class="c27"><p class="c4 c2"><a name="h.txhacg7bmkcp"></a><span>10</span></p></td><td class="c27"><p class="c4 c2"><a name="h.ruryne7tthly"></a><span>10</span></p></td><td class="c27"><p class="c4 c2"><a name="h.ph7l6nwj0gjg"></a><span>10</span></p></td></tr></tbody></table><p class="c1"><a name="h.7h62glaa4yxs"></a></p><p class="c1"><a name="h.yiydd1897x9l"></a></p><a href="#" name="6e3d096d4f19e3b479acad84230ddfd1ed51fb99"></a><a href="#" name="18"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c63"><p class="c4 c2"><a name="h.w5r95sgzz3de"></a><span>Digestion #2</span></p></td><td class="c84"><p class="c4 c2"><a name="h.shg4jq7tk7e6"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.i4tyvwlnt2cz"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.4e8wcbhlw2uf"></a><span>3</span></p></td><td class="c32"><p class="c4 c2"><a name="h.yy6qr0cckv8x"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.y2dm5eoypfl7"></a><span>5</span></p></td><td class="c32"><p class="c4 c2"><a name="h.ej3acoipgytk"></a><span>6</span></p></td><td class="c32"><p class="c4 c2"><a name="h.9c0kciymnagl"></a><span>7</span></p></td><td class="c32"><p class="c4 c2"><a name="h.waz255hd9f70"></a><span>8</span></p></td></tr><tr class="c8"><td class="c63"><p class="c4 c2"><a name="h.25rke0gez3u"></a><span>DNA</span></p></td><td class="c84"><p class="c4 c2"><a name="h.d1o0e83sow7k"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.g0jcy4txksbh"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.sjk3vdrjgr0i"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.s769fou19dqv"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.wofmxmlpgegu"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.490r9syon6yy"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.uvj00hgmzve4"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.yfgfb24q47wv"></a><span>4</span></p></td></tr><tr class="c8"><td class="c63"><p class="c4 c2"><a name="h.yxfyh25kr"></a><span>Buffer</span></p></td><td class="c84"><p class="c4 c2"><a name="h.17jjqi4cko9w"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.tbbryu1fptjw"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.kil451gf68zb"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.6bthosuis2ld"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.5ruryk190l6y"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.otjq8bsm9qzy"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.p0coi219v6fu"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.sxsz8pmiwxo4"></a><span>2</span></p></td></tr><tr class="c8"><td class="c63"><p class="c4 c2"><a name="h.yo40h58dwy19"></a><span>EcoR</span></p></td><td class="c84"><p class="c4 c2"><a name="h.k66gxom0sihr"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.adf4asiw7tc8"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.3nnvtvje8bt3"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.ml3rbpcgyxov"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.kfp0hy18jq7t"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.3bvz2np9788h"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.xtmaj5gtpije"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.oxd61apfk9qv"></a><span>1</span></p></td></tr><tr class="c8"><td class="c63"><p class="c4 c2"><a name="h.irelmh3yosec"></a><span>PstI</span></p></td><td class="c84"><p class="c4 c2"><a name="h.s3uaas3bjpb0"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.uo5q6iu6tjjc"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.z9n2nm7tlijj"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.ncu2jl8fqkt"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.w1tglhjopah9"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.lbrsjtd160he"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.g7kckqak8syq"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.4gkr4c91nlt2"></a><span>1</span></p></td></tr><tr class="c8"><td class="c63"><p class="c4 c2"><a name="h.83pjd4a6h35b"></a><span>Water</span></p></td><td class="c84"><p class="c4 c2"><a name="h.36d3xu7uq87m"></a><span>12</span></p></td><td class="c32"><p class="c4 c2"><a name="h.8j42nnal03uu"></a><span>12</span></p></td><td class="c32"><p class="c4 c2"><a name="h.qleutk3745uj"></a><span>12</span></p></td><td class="c32"><p class="c4 c2"><a name="h.ymia2hk4ktkj"></a><span>12</span></p></td><td class="c32"><p class="c4 c2"><a name="h.xtg7amrq798x"></a><span>12</span></p></td><td class="c32"><p class="c4 c2"><a name="h.oe3428yq07hx"></a><span>12</span></p></td><td class="c32"><p class="c4 c2"><a name="h.i3rsk29jxrhj"></a><span>12</span></p></td><td class="c32"><p class="c4 c2"><a name="h.jsd4zv9zl3rh"></a><span>12</span></p></td></tr><tr class="c8"><td class="c63"><p class="c4 c2"><a name="h.e6x30jr1n0q3"></a><span>Total</span></p></td><td class="c84"><p class="c4 c2"><a name="h.1vg74tppjqxe"></a><span>20</span></p></td><td class="c32"><p class="c4 c2"><a name="h.efr8py7hav4"></a><span>20</span></p></td><td class="c32"><p class="c4 c2"><a name="h.vz36gqyrwpjw"></a><span>20</span></p></td><td class="c32"><p class="c4 c2"><a name="h.gc3aj52yo3wh"></a><span>20</span></p></td><td class="c32"><p class="c4 c2"><a name="h.bbbs8h13lh35"></a><span>20</span></p></td><td class="c32"><p class="c4 c2"><a name="h.47r73jwcbtx4"></a><span>20</span></p></td><td class="c32"><p class="c4 c2"><a name="h.9gu3hni0qj2k"></a><span>20</span></p></td><td class="c32"><p class="c4 c2"><a name="h.rsfilo4bltd4"></a><span>20</span></p></td></tr></tbody></table><p class="c1"><a name="h.kyc9zicsa89a"></a></p><p class="c2"><a name="h.luqq5le1czzf"></a><span></span></p>	+	<p class="c2"><span>8/1</span></p><p class="c2"><a name="h.gdt42dr1sitx"></a><span>Miniprep</span></p><p class="c2"><a name="h.k16mhl32quoi"></a><span>i5-16-1 [Com B]                7.4</span></p><p class="c2"><a name="h.ykgo7uctfizd"></a><span>i5-16-2 [Com B]                5.4</span></p><p class="c2"><a name="h.b5syoehwe5sd"></a><span>i5-16-3[Com B]                13.7</span></p><p class="c2"><a name="h.mfxia9twmnj"></a><span>i5-16-4 [Scr]                no growth </span></p><p class="c2"><a name="h.601z1zffypq"></a><span>i5-16-5 [Scr]                10.7</span></p><p class="c2"><a name="h.ycdqtoz6j1jh"></a><span>i5-16-6[Scr]                7.8</span></p><p class="c2"><a name="h.ajykp6mwqppf"></a><span>i5-16-7[pScr]                8.4</span></p><p class="c2"><a name="h.ne55wp3gkau3"></a><span>i5-16-8[pScr]                9.1</span></p><p class="c2"><a name="h.e13d54dyzft7"></a><span>i5-16-9[pScr]                10.6</span></p><p class="c2"><a name="h.txo346njagbb"></a><span>i5-16-10 [Cra]                19.9</span></p><p class="c2"><a name="h.c3473p5uadpe"></a><span>i5-16-11[Cra]                5.2</span></p><p class="c2"><a name="h.wbmb3g1s7ugf"></a><span>i5-16-12 [Cra]                6.4</span></p><p class="c2"><a name="h.kv0tfbo2jv8i"></a><span>i5-16-13[pFru]                15.3</span></p><p class="c2"><a name="h.j3t9j0u7xkkq"></a><span>i5-16-14[pFru]                5.4</span></p><p class="c2"><a name="h.ilxeqahhep0j"></a><span>i5-16-15[pFru]                5.3</span></p><p class="c2"><a name="h.iwp9ty6hlxs1"></a><span>i5-16-16[Com C]        2.4</span></p><p class="c2"><a name="h.n4f67hryzt17"></a><span>i5-16-17[Com C]        7.7</span></p><p class="c2"><a name="h.l8oo77q6fsr"></a><span>i5-16-18[Com C]        5.8</span></p><p class="c1"><a name="h.df02fsoc2gkk"></a></p><p class="c2"><a name="h.s0shikkagflk"></a><span>Concentrations too low</span></p><p class="c2"><a name="h.h0j00xjiuue9"></a><span>Redo digestion and ligation (Plan B)</span></p><p class="c1"><a name="h.7k5mc2xcbp6i"></a></p><p class="c2"><a name="h.1h2u7hs3ix23"></a><span>Digestion </span></p><p class="c1"><a name="h.g3z76xurwd39"></a></p><a href="#" name="fdd2c2cc1a30f21ef2d5a146b718253990fe43a8"></a><a href="#" name="13"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c67"><p class="c1 c4"><a name="h.nw7x4tp0kj95"></a></p></td><td class="c86"><p class="c4 c2"><a name="h.b77riogep97k"></a><span>i5-11-5 (100.9 ng/ul) [pLac-RBS-GFP]</span></p></td></tr><tr class="c8"><td class="c67"><p class="c4 c2"><a name="h.7x23o2q63rld"></a><span>DNA</span></p></td><td class="c86"><p class="c4 c2"><a name="h.xs95dkb0qv3g"></a><span>20</span></p></td></tr><tr class="c8"><td class="c67"><p class="c4 c2"><a name="h.qmbaclu4and5"></a><span>NEBuffer</span></p></td><td class="c86"><p class="c4 c2"><a name="h.i4e84vgkrcxx"></a><span>5</span></p></td></tr><tr class="c8"><td class="c67"><p class="c4 c2"><a name="h.9fr4omfw11ah"></a><span>EcoR</span></p></td><td class="c86"><p class="c4 c2"><a name="h.xp4lm573njg7"></a><span>1</span></p></td></tr><tr class="c8"><td class="c67"><p class="c4 c2"><a name="h.fnra7wygpvwz"></a><span>PstI</span></p></td><td class="c86"><p class="c4 c2"><a name="h.k3078dha6squ"></a><span>1</span></p></td></tr><tr class="c8"><td class="c67"><p class="c4 c2"><a name="h.uzdcyicnhuw5"></a><span>Water</span></p></td><td class="c86"><p class="c4 c2"><a name="h.peiv6cad45bh"></a><span>23</span></p></td></tr><tr class="c8"><td class="c67"><p class="c4 c2"><a name="h.wla559qqazui"></a><span>Total</span></p></td><td class="c86"><p class="c4 c2"><a name="h.snx6esd4tjh1"></a><span>50 → i5-17-1</span></p></td></tr></tbody></table><p class="c1"><a name="h.hxsbfqaq1flk"></a></p><p class="c2"><a name="h.ak8khbywm050"></a><span>Digestion</span></p><p class="c1"><a name="h.qpk8acfchyb7"></a></p><a href="#" name="7150480a806c7acfe65074c44a5a56b72df44278"></a><a href="#" name="14"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c42"><p class="c1 c4"><a name="h.plaqos35rnbu"></a></p></td><td class="c42"><p class="c4 c2"><a name="h.oduap9peuhwz"></a><span>i4-45-3</span></p></td><td class="c42"><p class="c4 c2"><a name="h.28slfgkpyrku"></a><span>i4-45-2</span></p></td><td class="c42"><p class="c4 c2"><a name="h.qm7ywscmvd1o"></a><span>i5-11-5 [pLac-RBS-GFP] </span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.2y6nakdykx4n"></a><span>DNA</span></p></td><td class="c42"><p class="c4 c2"><a name="h.nluimw5ad5ii"></a><span>20</span></p></td><td class="c42"><p class="c4 c2"><a name="h.euarp7r4qqr4"></a><span>20</span></p></td><td class="c42"><p class="c4 c2"><a name="h.u7pjsz33yp8u"></a><span>20</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.fnxqh2ibdalu"></a><span>NEBuffer</span></p></td><td class="c42"><p class="c4 c2"><a name="h.kme0h754mt8u"></a><span>5</span></p></td><td class="c42"><p class="c4 c2"><a name="h.pychv9g0t5aj"></a><span>5</span></p></td><td class="c42"><p class="c4 c2"><a name="h.90qha3jcqgai"></a><span>5</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.xp8k4bpokxpe"></a><span>EcoR</span></p></td><td class="c42"><p class="c4 c2"><a name="h.simmuu8727lv"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.d4s682l63gvk"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.g0zvv2bkjcot"></a><span>1</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.lctki6qvqypt"></a><span>PstI</span></p></td><td class="c42"><p class="c4 c2"><a name="h.mvrdl2k9tfm"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.e1qgxndsr3jw"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.pcbwtt3jlera"></a><span>1</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.kn30n1rxa7pm"></a><span>Water</span></p></td><td class="c42"><p class="c4 c2"><a name="h.hfipwz68ut8i"></a><span>23</span></p></td><td class="c42"><p class="c4 c2"><a name="h.9a1getpgeol4"></a><span>23</span></p></td><td class="c42"><p class="c4 c2"><a name="h.xfxc3bw7xdpb"></a><span>23</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.7ubx7vr86r15"></a><span>Total</span></p></td><td class="c42"><p class="c4 c2"><a name="h.2fqkbpkycpxr"></a><span>50</span></p></td><td class="c42"><p class="c4 c2"><a name="h.cd74i22n9ixv"></a><span>50</span></p></td><td class="c42"><p class="c4 c2"><a name="h.2thgvsmqsnes"></a><span>50</span></p></td></tr></tbody></table><p class="c1"><a name="h.rnkzjpj51cq2"></a></p><p class="c2"><a name="h.462qh4ufcpyq"></a><span>Nanodrop i4-45-2        18.4</span></p><p class="c2"><a name="h.qymzp02bzgjz"></a><span>        i4-45-3                28.8</span></p><p class="c1"><a name="h.58tertazj0wl"></a></p><p class="c2"><a name="h.8yjdiim94mo2"></a><span>Ligation</span></p><p class="c2"><a name="h.odd5alle0l7u"></a><span>Mastermix: Vector: 16         Buffer: 16</span></p><p class="c1"><a name="h.9ljnk7muefc"></a></p><p class="c2"><a name="h.750mg81plwxp"></a><span>Vector: i4-45-3</span></p><p class="c1"><a name="h.qky6dflkxf5u"></a></p><a href="#" name="d7efde7569928a4e7ee8fc236d3137185b88940d"></a><a href="#" name="15"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.ko16dp6g3zlu"></a><span>Insert</span></p></td><td class="c35"><p class="c4 c2"><a name="h.g9oyoz6rhqne"></a><span>i5-13-9</span></p></td><td class="c35"><p class="c4 c2"><a name="h.ko16dp6g3zlu"></a><span>i5-13-9</span></p></td><td class="c35"><p class="c4 c2"><a name="h.ko16dp6g3zlu"></a><span>i5-13-10</span></p></td><td class="c35"><p class="c4 c2"><a name="h.g9oyoz6rhqne"></a><span>i5-13-11</span></p></td><td class="c35"><p class="c4 c2"><a name="h.hgf7vl6itpa4"></a><span>i5-13-12</span></p></td><td class="c35"><p class="c4 c2"><a name="h.ke9rrrsnsyde"></a><span>i5-13-13</span></p></td><td class="c35"><p class="c4 c2"><a name="h.1w7eymqeeklq"></a><span>i5-15-1</span></p></td></tr><tr class="c8"><td class="c35"><p class="c1 c4"><a name="h.ldquz8vbyhe6"></a></p></td><td class="c35"><p class="c4 c2"><a name="h.htbyr1ll042h"></a><span>3:1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.qlmj2ypxhaup"></a><span>0:1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.xb0vn1n1rtbt"></a><span>3:1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.ws93oogubgzu"></a><span>3:1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.n5puy2qf1f8"></a><span>3:1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.oikpvcs0iovu"></a><span>3:1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.1hj1ujr3oyqe"></a><span>3:1</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.gnq4j8mok285"></a><span>Insert</span></p></td><td class="c35"><p class="c4 c2"><a name="h.p7qkdz74u435"></a><span>7.5</span></p></td><td class="c35"><p class="c4 c2"><a name="h.do5uf7x3xfce"></a><span>0</span></p></td><td class="c35"><p class="c4 c2"><a name="h.gjadgexwrr8k"></a><span>6</span></p></td><td class="c35"><p class="c4 c2"><a name="h.qv4hhw4bo7xq"></a><span>1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.dj2b0xeslc0r"></a><span>5</span></p></td><td class="c35"><p class="c4 c2"><a name="h.wxppe7l5ijoc"></a><span>1.5</span></p></td><td class="c35"><p class="c4 c2"><a name="h.4rr06z9x2bi2"></a><span>1</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.6nrozzpemgsl"></a><span>Vector</span></p></td><td class="c35"><p class="c4 c2"><a name="h.294k89lar7ku"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.t20x9jisxas4"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.ihz5jvqdk19p"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.mlguz32wkiza"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.thhdugjt5v1a"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.6iohv0przw1"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.t36qi49njmay"></a><span>2</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.ix5ibc2nn2bl"></a><span>Buffer</span></p></td><td class="c35"><p class="c4 c2"><a name="h.rbbw0rx8m512"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.bg1sshpu7d3p"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.dx7eibvymd2p"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.ssu9fv79la3s"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.nmchd34dvlrd"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.o91ingua0t1m"></a><span>2</span></p></td><td class="c35"><p class="c4 c2"><a name="h.67y7n43zvoxa"></a><span>2</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.l9lmdir3u5kt"></a><span>Ligase</span></p></td><td class="c35"><p class="c4 c2"><a name="h.y303ooloe8eo"></a><span>1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.f7r14f39zeo9"></a><span>1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.3guxuh1ts81v"></a><span>1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.fco4l7y7m9i0"></a><span>1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.102mclq61u6e"></a><span>1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.wlk08oxitdt"></a><span>1</span></p></td><td class="c35"><p class="c4 c2"><a name="h.f76a5efgbwy0"></a><span>1</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.hkl9t5ohw35f"></a><span>water</span></p></td><td class="c35"><p class="c4 c2"><a name="h.unws8s6zidez"></a><span>7.5</span></p></td><td class="c35"><p class="c4 c2"><a name="h.lot17dn25adf"></a><span>15</span></p></td><td class="c35"><p class="c4 c2"><a name="h.pjp8seiw7ctd"></a><span>9</span></p></td><td class="c35"><p class="c4 c2"><a name="h.2njbhf4869si"></a><span>14</span></p></td><td class="c35"><p class="c4 c2"><a name="h.azmz7p4j1n6j"></a><span>10</span></p></td><td class="c35"><p class="c4 c2"><a name="h.i79hrut5w68k"></a><span>13.5</span></p></td><td class="c35"><p class="c4 c2"><a name="h.kuwi6l8k42ow"></a><span>14</span></p></td></tr><tr class="c8"><td class="c35"><p class="c4 c2"><a name="h.j2utgt1mtyc9"></a><span>Total</span></p></td><td class="c35"><p class="c4 c2"><a name="h.w0wf66iqe0tz"></a><span>20</span></p></td><td class="c35"><p class="c4 c2"><a name="h.7zdyt1ohkrtq"></a><span>20</span></p></td><td class="c35"><p class="c4 c2"><a name="h.l8d72odxorcw"></a><span>20</span></p></td><td class="c35"><p class="c4 c2"><a name="h.i3y4oas8rm7p"></a><span>20</span></p></td><td class="c35"><p class="c4 c2"><a name="h.jrhfboomo7ic"></a><span>20</span></p></td><td class="c35"><p class="c4 c2"><a name="h.7tkqll8rjzny"></a><span>20</span></p></td><td class="c35"><p class="c4 c2"><a name="h.7vhzegboowg7"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.u3kbvtk7cxk"></a><span>1                2                3                4                5                6                7</span></p><p class="c1"><a name="h.mt6betv3gsga"></a></p><p class="c2"><a name="h.vdlf9hfxs3za"></a><span>8/1</span></p><p class="c2"><a name="h.3kl77r7ego4"></a><span>Transformation</span></p><p class="c2"><a name="h.qnxw5fcp5bdl"></a><span>+ control → i5-10-4</span></p><p class="c1"><a name="h.rxixlc9jyz7j"></a></p><p class="c2"><a name="h.d3hn90r6uogh"></a><span>1 → 10 colonies → picked 2 → i5-19-1</span></p><p class="c2"><a name="h.tky8lnz5box"></a><span>                                i5-19-2</span></p><p class="c2"><a name="h.v9a0276oa2c3"></a><span>2 →0 colonies</span></p><p class="c2"><a name="h.8rg3vllobj6l"></a><span>3 →0 colonies</span></p><p class="c2"><a name="h.terx6m36at4p"></a><span>4 →20 colonies → picked 2 → i5-19-3</span></p><p class="c2"><a name="h.ax82ddgvej52"></a><span>                                i5-19-4</span></p><p class="c2"><a name="h.kl8f3p7w9a94"></a><span>5 →50 + colonies → picked 2 → i5-19-5</span></p><p class="c2"><a name="h.rly6ll3758vh"></a><span>                                i5-19-6</span></p><p class="c2"><a name="h.t6yhc2bwlkmr"></a><span>6 →0 colonies (ruined plate)</span></p><p class="c2"><a name="h.txw92pj0umww"></a><span>7 →25  colonies → picked 2 → i5-19-7</span></p><p class="c2"><a name="h.2pg34ohcmptq"></a><span>                                i5-19-8</span></p><p class="c1"><a name="h.t1r4u7513way"></a></p><p class="c2"><a name="h.1yh2ap6r5dkj"></a><span>8/2</span></p><p class="c2"><a name="h.cbb9vyy27u50"></a><span>Transformation</span></p><p class="c2"><a name="h.tkzkibvuxed7"></a><span>+ control → i5-10-4</span></p><p class="c2"><a name="h.ygzhfj4d971e"></a><span>1 → 6 colonies → picked 2 → i5-19-9</span></p><p class="c2"><a name="h.jkleuycfz7gw"></a><span>                                i5-19-10</span></p><p class="c2"><a name="h.92goxt1940i6"></a><span>3 → 20 colonies → picked 2 → i5-19-11</span></p><p class="c2"><a name="h.n4keacizzoq7"></a><span>                                i5-19-12</span></p><p class="c2"><a name="h.1657iecoccwd"></a><span>6→ 8 colonies → picked 2 → i5-19-13</span></p><p class="c2"><a name="h.o4r84ljldi8j"></a><span>                                i5-19-14</span></p><p class="c1"><a name="h.i28qtsm3t7e"></a></p><p class="c2"><a name="h.hupfbsd3ivci"></a><span>8/3 </span></p><p class="c2"><a name="h.h7xzg2fhesfu"></a><span>Miniprep </span></p><p class="c1"><a name="h.785x68lq4t23"></a></p><a href="#" name="5da75b8ad68cb98f581dda94edaf46683d1aed71"></a><a href="#" name="16"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c5"><p class="c1 c4"><a name="h.5324zw47di0h"></a></p></td><td class="c48"><p class="c4 c2"><a name="h.i1tf65pvidmt"></a><span>Concentrations ng/ul</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.v7qvzht8rovt"></a><span>i5-19-1 [Com B]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.oycn5t5zwqw"></a><span>90.3</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.yg0vhvy9xbin"></a><span>i5-19-2 [Com B]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.6y6ofdr2i8h0"></a><span>77.5</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.ykmyu8l6hcn2"></a><span>i5-19-3 [pScr]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.3mr1227qxaog"></a><span>39.6</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.psdo7yxrgz7u"></a><span>i5-19-4 [pScr]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.d2gmy5rmmpa1"></a><span>89.5</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.whyjlta6ggiw"></a><span>i5-19-5 [Cra]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.nvrasbi1dfp7"></a><span>71.6</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.2adme0o5ap57"></a><span>i5-19-6 [Cra]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.jfs9zfjou67u"></a><span>120.2</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.g3ggqz1i48at"></a><span>i5-19-7 [Com C]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.zieop85xawgl"></a><span>124.8</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.al7jvioy9ey8"></a><span>i5-19-8 [Com C]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.qquo1hy4hm57"></a><span>101.6</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.sqlxlp2zkwfk"></a><span>i5-19-9</span></p></td><td class="c48"><p class="c4 c2"><a name="h.v2yehdjyl87j"></a><span>108.9</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.o2jm3ibgeqzn"></a><span>i5-19-10</span></p></td><td class="c48"><p class="c4 c2"><a name="h.r9qkczavx1zz"></a><span>102.3</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.3sd8ogw68ews"></a><span>i5-19-11 [Scr]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.t04wha5b9m3c"></a><span>85.8</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.toa1hyt6erb"></a><span>i5-19-12</span></p></td><td class="c48"><p class="c4 c2"><a name="h.l2uogxg89tml"></a><span>86.9</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.xyabvc51wqmz"></a><span>i5-19-13 [pFru]</span></p></td><td class="c48"><p class="c4 c2"><a name="h.t6elcnx8txdu"></a><span>101.4</span></p></td></tr><tr class="c8"><td class="c5"><p class="c4 c2"><a name="h.uwy9t1l2s2f1"></a><span>i5-19-14</span></p></td><td class="c48"><p class="c4 c2"><a name="h.zgdrxg3hnv3k"></a><span>97.8</span></p></td></tr></tbody></table><p class="c1"><a name="h.cb1zi8x4xf3h"></a></p><p class="c1"><a name="h.podivfsbn9ht"></a></p><a href="#" name="e792f2144a5f6b55832d2e140860bcc0027fa187"></a><a href="#" name="17"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c27"><p class="c4 c2"><a name="h.oiv53yapn8kh"></a><span>Digestion</span></p></td><td class="c27"><p class="c4 c2"><a name="h.ou4hb7h0seu9"></a><span>i5-19-1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.qte7npmt8r5c"></a><span>i5-19-2</span></p></td><td class="c27"><p class="c4 c2"><a name="h.t9s41xvjzho"></a><span>i5-19-3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.40i1l3i78is1"></a><span>i5-19-4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.br6zg9exyqka"></a><span>i5-19-5</span></p></td><td class="c27"><p class="c4 c2"><a name="h.ii3zqc2ors8j"></a><span>i5-19-6</span></p></td><td class="c27"><p class="c4 c2"><a name="h.fnvbqzaz7j1v"></a><span>i5-19-7</span></p></td><td class="c27"><p class="c4 c2"><a name="h.foy8blyca3l"></a><span>i5-19-8</span></p></td></tr><tr class="c8"><td class="c27"><p class="c4 c2"><a name="h.rf3ds9ps6nzd"></a><span>DNA</span></p></td><td class="c27"><p class="c4 c2"><a name="h.zfo8ozunugrr"></a><span>4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.68oznqb11yif"></a><span>4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.1cme7yxrlqj6"></a><span>4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.5l2uwmewg7ig"></a><span>4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.58m87rj49d9u"></a><span>4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.kvb2741eni0g"></a><span>4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.lsdtxms32y8a"></a><span>4</span></p></td><td class="c27"><p class="c4 c2"><a name="h.c1b212wrflbg"></a><span>4</span></p></td></tr><tr class="c8"><td class="c27"><p class="c4 c2"><a name="h.xikbro4ycyy1"></a><span>Buffer</span></p></td><td class="c27"><p class="c4 c2"><a name="h.vqsvtvp4raop"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.jqxro38sinut"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.2wn1xtn04gdc"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.esnrwhx0uqms"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.ug391abaxoi8"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.4n9bhe83qr01"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.v2f5x9dp0y5s"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.905i5wpg00mj"></a><span>1</span></p></td></tr><tr class="c8"><td class="c27"><p class="c4 c2"><a name="h.ihwa2erhrsb"></a><span>EcoR</span></p></td><td class="c27"><p class="c4 c2"><a name="h.41npynb4vjfh"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.guc33o4xo1ny"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.70bbc5qgkzwy"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.m2ea79t163dd"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.o4d1umcs55jw"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.rl19xxze3i3y"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.nh58fgbq8r36"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.mi69vlcxfy8l"></a><span>1</span></p></td></tr><tr class="c8"><td class="c27"><p class="c4 c2"><a name="h.mwpm9bcdyuz"></a><span>PstI</span></p></td><td class="c27"><p class="c4 c2"><a name="h.pbvtqamppw6t"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.gbl8y6odfyfe"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.8adi8qp99j8w"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.260azt8lkiok"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.41nqramq4vdp"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.gad2myviufeq"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.8cusr7ukuysz"></a><span>1</span></p></td><td class="c27"><p class="c4 c2"><a name="h.kiiioy1rzgw8"></a><span>1</span></p></td></tr><tr class="c8"><td class="c27"><p class="c4 c2"><a name="h.41vrfxo8n7q1"></a><span>Water</span></p></td><td class="c27"><p class="c4 c2"><a name="h.dk5jox3vckg2"></a><span>3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.a2vkupkprp96"></a><span>3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.5dsl3xrxgwhv"></a><span>3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.c860uhtzb5c"></a><span>3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.iyx6d43bxlro"></a><span>3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.meo2phn740du"></a><span>3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.x8ehq55mafpt"></a><span>3</span></p></td><td class="c27"><p class="c4 c2"><a name="h.nw7dgbn47q03"></a><span>3</span></p></td></tr><tr class="c8"><td class="c27"><p class="c4 c2"><a name="h.k9qik7fcg9bf"></a><span>Total</span></p></td><td class="c27"><p class="c4 c2"><a name="h.cul31bek5537"></a><span>10</span></p></td><td class="c27"><p class="c4 c2"><a name="h.f1xx1r7dzjjr"></a><span>10</span></p></td><td class="c27"><p class="c4 c2"><a name="h.ba6wq53qokt0"></a><span>10</span></p></td><td class="c27"><p class="c4 c2"><a name="h.41opoprgaair"></a><span>10</span></p></td><td class="c27"><p class="c4 c2"><a name="h.1ncbe75oart8"></a><span>10</span></p></td><td class="c27"><p class="c4 c2"><a name="h.txhacg7bmkcp"></a><span>10</span></p></td><td class="c27"><p class="c4 c2"><a name="h.ruryne7tthly"></a><span>10</span></p></td><td class="c27"><p class="c4 c2"><a name="h.ph7l6nwj0gjg"></a><span>10</span></p></td></tr></tbody></table><p class="c1"><a name="h.7h62glaa4yxs"></a></p><p class="c1"><a name="h.yiydd1897x9l"></a></p><a href="#" name="6e3d096d4f19e3b479acad84230ddfd1ed51fb99"></a><a href="#" name="18"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c63"><p class="c4 c2"><a name="h.w5r95sgzz3de"></a><span>Digestion #2</span></p></td><td class="c84"><p class="c4 c2"><a name="h.shg4jq7tk7e6"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.i4tyvwlnt2cz"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.4e8wcbhlw2uf"></a><span>3</span></p></td><td class="c32"><p class="c4 c2"><a name="h.yy6qr0cckv8x"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.y2dm5eoypfl7"></a><span>5</span></p></td><td class="c32"><p class="c4 c2"><a name="h.ej3acoipgytk"></a><span>6</span></p></td><td class="c32"><p class="c4 c2"><a name="h.9c0kciymnagl"></a><span>7</span></p></td><td class="c32"><p class="c4 c2"><a name="h.waz255hd9f70"></a><span>8</span></p></td></tr><tr class="c8"><td class="c63"><p class="c4 c2"><a name="h.25rke0gez3u"></a><span>DNA</span></p></td><td class="c84"><p class="c4 c2"><a name="h.d1o0e83sow7k"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.g0jcy4txksbh"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.sjk3vdrjgr0i"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.s769fou19dqv"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.wofmxmlpgegu"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.490r9syon6yy"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.uvj00hgmzve4"></a><span>4</span></p></td><td class="c32"><p class="c4 c2"><a name="h.yfgfb24q47wv"></a><span>4</span></p></td></tr><tr class="c8"><td class="c63"><p class="c4 c2"><a name="h.yxfyh25kr"></a><span>Buffer</span></p></td><td class="c84"><p class="c4 c2"><a name="h.17jjqi4cko9w"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.tbbryu1fptjw"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.kil451gf68zb"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.6bthosuis2ld"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.5ruryk190l6y"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.otjq8bsm9qzy"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.p0coi219v6fu"></a><span>2</span></p></td><td class="c32"><p class="c4 c2"><a name="h.sxsz8pmiwxo4"></a><span>2</span></p></td></tr><tr class="c8"><td class="c63"><p class="c4 c2"><a name="h.yo40h58dwy19"></a><span>EcoR</span></p></td><td class="c84"><p class="c4 c2"><a name="h.k66gxom0sihr"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.adf4asiw7tc8"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.3nnvtvje8bt3"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.ml3rbpcgyxov"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.kfp0hy18jq7t"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.3bvz2np9788h"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.xtmaj5gtpije"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.oxd61apfk9qv"></a><span>1</span></p></td></tr><tr class="c8"><td class="c63"><p class="c4 c2"><a name="h.irelmh3yosec"></a><span>PstI</span></p></td><td class="c84"><p class="c4 c2"><a name="h.s3uaas3bjpb0"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.uo5q6iu6tjjc"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.z9n2nm7tlijj"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.ncu2jl8fqkt"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.w1tglhjopah9"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.lbrsjtd160he"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.g7kckqak8syq"></a><span>1</span></p></td><td class="c32"><p class="c4 c2"><a name="h.4gkr4c91nlt2"></a><span>1</span></p></td></tr><tr class="c8"><td class="c63"><p class="c4 c2"><a name="h.83pjd4a6h35b"></a><span>Water</span></p></td><td class="c84"><p class="c4 c2"><a name="h.36d3xu7uq87m"></a><span>12</span></p></td><td class="c32"><p class="c4 c2"><a name="h.8j42nnal03uu"></a><span>12</span></p></td><td class="c32"><p class="c4 c2"><a name="h.qleutk3745uj"></a><span>12</span></p></td><td class="c32"><p class="c4 c2"><a name="h.ymia2hk4ktkj"></a><span>12</span></p></td><td class="c32"><p class="c4 c2"><a name="h.xtg7amrq798x"></a><span>12</span></p></td><td class="c32"><p class="c4 c2"><a name="h.oe3428yq07hx"></a><span>12</span></p></td><td class="c32"><p class="c4 c2"><a name="h.i3rsk29jxrhj"></a><span>12</span></p></td><td class="c32"><p class="c4 c2"><a name="h.jsd4zv9zl3rh"></a><span>12</span></p></td></tr><tr class="c8"><td class="c63"><p class="c4 c2"><a name="h.e6x30jr1n0q3"></a><span>Total</span></p></td><td class="c84"><p class="c4 c2"><a name="h.1vg74tppjqxe"></a><span>20</span></p></td><td class="c32"><p class="c4 c2"><a name="h.efr8py7hav4"></a><span>20</span></p></td><td class="c32"><p class="c4 c2"><a name="h.vz36gqyrwpjw"></a><span>20</span></p></td><td class="c32"><p class="c4 c2"><a name="h.gc3aj52yo3wh"></a><span>20</span></p></td><td class="c32"><p class="c4 c2"><a name="h.bbbs8h13lh35"></a><span>20</span></p></td><td class="c32"><p class="c4 c2"><a name="h.47r73jwcbtx4"></a><span>20</span></p></td><td class="c32"><p class="c4 c2"><a name="h.9gu3hni0qj2k"></a><span>20</span></p></td><td class="c32"><p class="c4 c2"><a name="h.rsfilo4bltd4"></a><span>20</span></p></td></tr></tbody></table><p class="c1"><a name="h.kyc9zicsa89a"></a></p><p class="c2"><a name="h.luqq5le1czzf"></a><span>8/4</span></p><p class="c1"><a name="h.sgg25dthi5y5"></a></p><a href="#" name="b65b6d34fbf5c5fb5aaaa4002212f34d7190e2a0"></a><a href="#" name="19"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.a5jijuql1rvr"></a><span>Digestion</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ezp4ebrmrt24"></a><span>i5-19-9</span></p></td><td class="c13"><p class="c4 c2"><a name="h.iabe8cgq9hmu"></a><span>i5-19-10</span></p></td><td class="c13"><p class="c4 c2"><a name="h.7yg7kzgsqtkh"></a><span>i5-19-11</span></p></td><td class="c13"><p class="c4 c2"><a name="h.3q6b1wcbissm"></a><span>i5-19-12</span></p></td><td class="c13"><p class="c4 c2"><a name="h.k8ue8imb8tnj"></a><span>i5-19-13</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ma78axahim7x"></a><span>i5-19-14</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.61t44pohvsaq"></a><span>DNA</span></p></td><td class="c13"><p class="c4 c2"><a name="h.x56fdk4v3ovw"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.2hvapkiw4m87"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.6f886gfut9m0"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.h8oscj46xqwu"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.kbbpg5ihzoll"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.my8jpea79q93"></a><span>2</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.w9esfgelg75h"></a><span>Buffer</span></p></td><td class="c13"><p class="c4 c2"><a name="h.cfww47qpd4af"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.qdl9k4lnp4su"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.abiben5rcnr3"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.3qcfojjf5lcr"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.w7s2l12kuc50"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.dzu83mp3t41"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.af2xg2y7avl3"></a><span>EcoR</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ni5uft1ulrul"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.1wo6olhwpjw2"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.wchoy4dr7x4g"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.tbg88l1b6cy2"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.c5y5gdkqyue2"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.1am4iyn15gpa"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.4tfklkehn3i5"></a><span>PstI</span></p></td><td class="c13"><p class="c4 c2"><a name="h.y5p3h0ubvj0y"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.6i9m52boa53p"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.13m0x4keo2uy"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.yfvo3v5lne2u"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.l32zngxtccv"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.xiw4zxi2jl9m"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.zi71ebctrsd"></a><span>Water</span></p></td><td class="c13"><p class="c4 c2"><a name="h.d0clbz3fd71r"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ryzpdn4j22ul"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.gmgufotxnxut"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.kp5bilx6wvit"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.3vycwm7wlzip"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.6c3d5q4bnsd1"></a><span>5</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.pfexr4rksv9i"></a><span>Total</span></p></td><td class="c13"><p class="c4 c2"><a name="h.xs9j65pjtnxv"></a><span>10</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ayq5fs1mxbjc"></a><span>10</span></p></td><td class="c13"><p class="c4 c2"><a name="h.pk8u9by8feav"></a><span>10</span></p></td><td class="c13"><p class="c4 c2"><a name="h.aou9icqk61cv"></a><span>10</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ez4mpw3kmvv0"></a><span>10</span></p></td><td class="c13"><p class="c4 c2"><a name="h.vfyzzhbb5ljt"></a><span>10</span></p></td></tr></tbody></table><p class="c1"><a name="h.2yrznbpl1lfj"></a></p><p class="c2"><a name="h.o6k1q9vzjd7a"></a><span>8/5 </span></p><p class="c2"><a name="h.2jmyh9gl83yq"></a><span>Digestion</span></p><p class="c1"><a name="h.ii8nthcymtpb"></a></p><a href="#" name="5d3e127f652e674bf47a39df42206f4c48805de0"></a><a href="#" name="20"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c13"><p class="c1 c4"><a name="h.a6dix5iagyun"></a></p></td><td class="c13"><p class="c4 c2"><a name="h.n9qgtg7uhhub"></a><span>i5-19-1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.x3s5avd5kmni"></a><span>i5-19-3</span></p></td><td class="c13"><p class="c4 c2"><a name="h.4gq7ivf6m0v5"></a><span>i5-19-5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.73crz0713h49"></a><span>i5-19-7</span></p></td><td class="c13"><p class="c4 c2"><a name="h.6nra6sudasj9"></a><span>i5-19-11</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ao2ac858ohn1"></a><span>i5-19-13</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.ooejk4dkc05a"></a><span>DNA</span></p></td><td class="c13"><p class="c4 c2"><a name="h.wjvlwqn0s8dc"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.eeg76xr9b7fv"></a><span>12.5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.pfzeo3ypcsto"></a><span>7.1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.qaoefpqrj4d7"></a><span>4</span></p></td><td class="c13"><p class="c4 c2"><a name="h.qu57tb5cdrok"></a><span>5.8</span></p></td><td class="c13"><p class="c4 c2"><a name="h.hgttgxoa6ab0"></a><span>5</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.e2razpc3mzde"></a><span>Buffer</span></p></td><td class="c13"><p class="c4 c2"><a name="h.f6uav9yzdyuy"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.4aqyawqr5qv3"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.b6rdcctzd01d"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.g5zt6eo2s21p"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.9skiq0maxx9b"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.qk65utt0ptcm"></a><span>2</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.4bnct5urijfx"></a><span>EcoR</span></p></td><td class="c13"><p class="c4 c2"><a name="h.33oeogcp86tv"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.jbie2odvtq7x"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.xblh5q1t1us0"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.hkms45pn6iwi"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.3j60bs4b7afo"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.l9onmyyqpe7z"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.401yo1d7kmd3"></a><span>PstI</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ifx7lnwalcxl"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.bw9e2h26z0uf"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.dnizgujui6pw"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.x5v4qjvauhyc"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.stev3lmw8nlg"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.tt6o8acvxkx"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.x6hciqcwfojq"></a><span>Water</span></p></td><td class="c13"><p class="c4 c2"><a name="h.9ht31r5zgfvd"></a><span>11</span></p></td><td class="c13"><p class="c4 c2"><a name="h.gv4kxpd8xtgw"></a><span>3.5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.yp8bd29zeip0"></a><span>8.9</span></p></td><td class="c13"><p class="c4 c2"><a name="h.n372tyu3vdn2"></a><span>12</span></p></td><td class="c13"><p class="c4 c2"><a name="h.or1waswqa7s0"></a><span>10.2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.26z1dl5b9lkj"></a><span>11</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.eho85b950qz4"></a><span>Total</span></p></td><td class="c13"><p class="c4 c2"><a name="h.yfnq9ah2mle0"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.wqspntd4kqe0"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.2jm6j51z1lg"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.9q55arl12i8e"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.wdg4qulbn5ru"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.bgbd4xfuntjm"></a><span>20</span></p></td></tr></tbody></table><p class="c1"><a name="h.5zjr9xt41w5m"></a></p><a href="#" name="edfa04fda6ad415d6e2fba616fd8950368ba86e6"></a><a href="#" name="21"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c89"><p class="c1 c4"><a name="h.a6dix5iagyun"></a></p></td><td class="c30"><p class="c4 c2"><a name="h.n9qgtg7uhhub"></a><span>i5-19-1</span></p></td></tr><tr class="c8"><td class="c89"><p class="c4 c2"><a name="h.ooejk4dkc05a"></a><span>DNA</span></p></td><td class="c30"><p class="c4 c2"><a name="h.wjvlwqn0s8dc"></a><span>5</span></p></td></tr><tr class="c8"><td class="c89"><p class="c4 c2"><a name="h.e2razpc3mzde"></a><span>Buffer</span></p></td><td class="c30"><p class="c4 c2"><a name="h.f6uav9yzdyuy"></a><span>2</span></p></td></tr><tr class="c8"><td class="c89"><p class="c4 c2"><a name="h.4bnct5urijfx"></a><span>EcoR</span></p></td><td class="c30"><p class="c4 c2"><a name="h.33oeogcp86tv"></a><span>1</span></p></td></tr><tr class="c8"><td class="c89"><p class="c4 c2"><a name="h.401yo1d7kmd3"></a><span>PstI</span></p></td><td class="c30"><p class="c4 c2"><a name="h.ifx7lnwalcxl"></a><span>1</span></p></td></tr><tr class="c8"><td class="c89"><p class="c4 c2"><a name="h.x6hciqcwfojq"></a><span>Water</span></p></td><td class="c30"><p class="c4 c2"><a name="h.9ht31r5zgfvd"></a><span>11</span></p></td></tr><tr class="c8"><td class="c89"><p class="c4 c2"><a name="h.eho85b950qz4"></a><span>Total</span></p></td><td class="c30"><p class="c4 c2"><a name="h.yfnq9ah2mle0"></a><span>20</span></p></td></tr></tbody></table><p class="c1"><a name="h.9s6iusvs6p02"></a></p><p class="c2"><a name="h.31al3sts3g4k"></a><span>Picked colonies from transformation of 8/2 and 8/1</span></p><p class="c1"><a name="h.6koh1p1nz9b7"></a></p><p class="c2"><a name="h.fltmgetfd1cq"></a><span>Colonies from 8/1</span></p><p class="c2"><a name="h.ap81cglnf23i"></a><span>1→ i5-22-1         Com B</span></p><p class="c2"><a name="h.17hi9mgj55xx"></a><span>4→ i5-22-2        pScr        Made glycerol stock for all 6</span></p><p class="c2"><a name="h.l5rilafwggo2"></a><span>5→ i5-22-3         Cra</span></p><p class="c2"><a name="h.4rt4e9ch9m8g"></a><span>7→ i5-22-4         Com C</span></p><p class="c1"><a name="h.ibu662kjaieq"></a></p><p class="c2"><a name="h.mzbnml4va3h9"></a><span>Colonies from 8/2 </span></p><p class="c2"><a name="h.bt2c6b5iji0t"></a><span>3 → i5-22-5        SCr</span></p><p class="c2"><a name="h.am7rhjrwfeew"></a><span>6→ i5-22-5        pFru</span></p><p class="c1"><a name="h.3osb89fv4fo1"></a></p><p class="c2"><a name="h.n85mmvxms0xm"></a><span>8/6 </span></p><p class="c2"><a name="h.uhbw6a447c9c"></a><span>Miniprep        Concentrations (ng/ul)</span></p><p class="c2"><a name="h.u1c3fa9lxf43"></a><span>i5-22-1                67.9        Com B</span></p><p class="c2"><a name="h.2s16kmp8fwjo"></a><span>i5-22-2                53.0        pScr</span></p><p class="c2"><a name="h.x2riupq5fzvg"></a><span>i5-22-3                90.9        Cra</span></p><p class="c2"><a name="h.3lehouxtgx4g"></a><span>i5-22-4                68.1         Com C</span></p><p class="c2"><a name="h.ghmduw89ynii"></a><span>i5-22-5                101.8        Scr</span></p><p class="c2"><a name="h.lj0ad7dpkd0a"></a><span>i5-22-6                185.5        pFru</span></p><p class="c1"><a name="h.tamily566yxl"></a></p><p class="c2"><a name="h.2pzbef12bxzr"></a><span>Test Digestion</span></p><p class="c1"><a name="h.qnwry7b6xsb"></a></p><a href="#" name="810c30ac7ea7af7f46d0637a23252142e0dd376c"></a><a href="#" name="22"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c13"><p class="c1 c4"><a name="h.yb5evczhpuom"></a></p></td><td class="c13"><p class="c4 c2"><a name="h.38w6pbrpqzdp"></a><span>i5-22-1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.zgn0l6d6vb5t"></a><span>i5-22-2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.r0yng41r0sic"></a><span>i5-22-3</span></p></td><td class="c13"><p class="c4 c2"><a name="h.p87wenjmpjwd"></a><span>i5-22-4</span></p></td><td class="c13"><p class="c4 c2"><a name="h.gkiou64ws2yz"></a><span>i5-22-5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.v2g12flru3jf"></a><span>i5-22-6</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.va3lacp26cyj"></a><span>DNA</span></p></td><td class="c13"><p class="c4 c2"><a name="h.yg4cfww47xgo"></a><span>3</span></p></td><td class="c13"><p class="c4 c2"><a name="h.9tdm4gqjkkrq"></a><span>4</span></p></td><td class="c13"><p class="c4 c2"><a name="h.442vr9tpdgd"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.o8mgufjbn5t0"></a><span>2.8</span></p></td><td class="c13"><p class="c4 c2"><a name="h.dt9091mp8qd3"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ulcrx6jqi4nf"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.kcdjdapobt6l"></a><span>Cutsmart</span></p></td><td class="c13"><p class="c4 c2"><a name="h.elf3qphugt90"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.3aenyfvkyno8"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ngwgkceddqu3"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.vls4ubjauel4"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.j3boni8avurg"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.sofpjesu1oxp"></a><span>2</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.5ybhn2jvcnur"></a><span>EcoR-HF</span></p></td><td class="c13"><p class="c4 c2"><a name="h.85q2gdi4ggxr"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.twfsbe5gdm9"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.c1ss864a3wyk"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.nmpu7yj3iswf"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.h4x5nkmbjj16"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.8wblq31iu8g7"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.diecaj2350pk"></a><span>Spe I</span></p></td><td class="c13"><p class="c4 c2"><a name="h.431hxums39ls"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ub0o1kbmag9s"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.bt4rjiep29yt"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.z7prz5q61f4i"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.5zwv242rkfvi"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.5bb79vg9hwzd"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.6gpw0rpjdfqg"></a><span>Water</span></p></td><td class="c13"><p class="c4 c2"><a name="h.tgamgczid50z"></a><span>13</span></p></td><td class="c13"><p class="c4 c2"><a name="h.g66iquhf4yb2"></a><span>12</span></p></td><td class="c13"><p class="c4 c2"><a name="h.daevk46emsi9"></a><span>14</span></p></td><td class="c13"><p class="c4 c2"><a name="h.5ctf9kjxkhwb"></a><span>13.2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.chhppzctwrg1"></a><span>14</span></p></td><td class="c13"><p class="c4 c2"><a name="h.plsnqnkfaspd"></a><span>15</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.2r87y2owrqhc"></a><span>Total</span></p></td><td class="c13"><p class="c4 c2"><a name="h.cmo9e7puvjlm"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ag8h3mnqd2l7"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.p4uocxac3rwe"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.izmeh02q5kv7"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.k5xtypjrck2a"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.uyb7c1nwgy0u"></a><span>20</span></p></td></tr></tbody></table><p class="c1"><a name="h.tgwmyrwiukz7"></a></p><p class="c2"><a name="h.e4kpgf1mueqv"></a><span>Digestion of backbone (pLac-GFP i5-11-5)</span></p><p class="c1"><a name="h.stcgnprpfqqm"></a></p><a href="#" name="2ba8e1570a206c8c0cd15a29df10cc1f3dacbf85"></a><a href="#" name="23"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c44"><p class="c1 c4"><a name="h.d25ftzkasjyj"></a></p></td><td class="c16"><p class="c4 c2"><a name="h.vwcs3u4w170g"></a><span>Com C (54.1)</span></p></td><td class="c16"><p class="c4 c2"><a name="h.c6xlvkt0pe77"></a><span>Com D (46.4)</span></p></td><td class="c28"><p class="c4 c2"><a name="h.o4fv70lu3990"></a><span>ComE (84.3)</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.44ngp9sc0gsx"></a><span>DNA</span></p></td><td class="c16"><p class="c4 c2"><a name="h.roe6ssa2ml6y"></a><span>40</span></p></td><td class="c16"><p class="c4 c2"><a name="h.fdpi22eu3m0i"></a><span>20</span></p></td><td class="c28"><p class="c4 c2"><a name="h.vg5gqoz8kjjv"></a><span>25</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.q0d68ztwehmr"></a><span>Buffer</span></p></td><td class="c16"><p class="c4 c2"><a name="h.v3t9mcfcbb2p"></a><span>5</span></p></td><td class="c16"><p class="c4 c2"><a name="h.dyjmwex93rs8"></a><span>5</span></p></td><td class="c28"><p class="c4 c2"><a name="h.9okzlob9xvmu"></a><span>5</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.nca0yzv523b"></a><span>Eco R</span></p></td><td class="c16"><p class="c4 c2"><a name="h.vh9t3cy5bmo6"></a><span>1</span></p></td><td class="c16"><p class="c4 c2"><a name="h.b6x14t79v1k4"></a><span>0</span></p></td><td class="c28"><p class="c4 c2"><a name="h.np51w6d9b29k"></a><span>0</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.5ago9jf3relr"></a><span>PstI</span></p></td><td class="c16"><p class="c4 c2"><a name="h.gqfk1jvl3ux7"></a><span>0</span></p></td><td class="c16"><p class="c4 c2"><a name="h.8d23ozny1z0m"></a><span>0</span></p></td><td class="c28"><p class="c4 c2"><a name="h.85nkqupm56uz"></a><span>1</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.tqxwofs4qxbc"></a><span>XbaI</span></p></td><td class="c16"><p class="c4 c2"><a name="h.dwkx341va5rr"></a><span>0</span></p></td><td class="c16"><p class="c4 c2"><a name="h.b43lcolhjx74"></a><span>1</span></p></td><td class="c28"><p class="c4 c2"><a name="h.5235tbrpkcom"></a><span>1</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.pznia856efr"></a><span>SpcI</span></p></td><td class="c16"><p class="c4 c2"><a name="h.hf4euicphgpb"></a><span>1</span></p></td><td class="c16"><p class="c4 c2"><a name="h.yu17ovg1nn2k"></a><span>1</span></p></td><td class="c28"><p class="c4 c2"><a name="h.sip7erk41zf6"></a><span>0</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.wnwxgm43end6"></a><span>Water</span></p></td><td class="c16"><p class="c4 c2"><a name="h.zdgligi8l7q8"></a><span>3</span></p></td><td class="c16"><p class="c4 c2"><a name="h.cukagcofwo2c"></a><span>23</span></p></td><td class="c28"><p class="c4 c2"><a name="h.xn42hpzcmfmz"></a><span>18</span></p></td></tr><tr class="c8"><td class="c44"><p class="c4 c2"><a name="h.pvrd163duddt"></a><span>Total</span></p></td><td class="c16"><p class="c4 c2"><a name="h.i9ljeyx3xeak"></a><span>50</span></p></td><td class="c16"><p class="c4 c2"><a name="h.enwfnqcs2nbq"></a><span>50</span></p></td><td class="c28"><p class="c4 c2"><a name="h.j2h3yznwe43u"></a><span>50</span></p></td></tr></tbody></table><p class="c1"><a name="h.s31a92d1wvih"></a></p><p class="c2"><a name="h.y9wahg3dflx7"></a><span>Digestion of insert</span></p><p class="c1"><a name="h.ai7xrolf51jo"></a></p><a href="#" name="4cdf434ed1f855c880097220a3ba4c66edfc753a"></a><a href="#" name="24"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c52"><p class="c1 c4"><a name="h.uh8rvi2dflk4"></a></p></td><td class="c39"><p class="c4 c2"><a name="h.tf68l84o8uvo"></a><span>Com A</span></p></td><td class="c63"><p class="c4 c2"><a name="h.nz13dv1nbx1n"></a><span>Com D</span></p></td><td class="c90"><p class="c4 c2"><a name="h.bnddbpky3te0"></a><span>Com E</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.44ngp9sc0gsx"></a><span>DNA</span></p></td><td class="c39"><p class="c4 c2"><a name="h.25pbpv9anmf5"></a><span>30</span></p></td><td class="c63"><p class="c4 c2"><a name="h.z0agsj6mnyww"></a><span>30</span></p></td><td class="c90"><p class="c4 c2"><a name="h.ssbk3twkauok"></a><span>30</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.q0d68ztwehmr"></a><span>Buffer</span></p></td><td class="c39"><p class="c4 c2"><a name="h.z5j53jq0ix0"></a><span>5</span></p></td><td class="c63"><p class="c4 c2"><a name="h.gbddpo6rdg2a"></a><span>5</span></p></td><td class="c90"><p class="c4 c2"><a name="h.y981msirfbq4"></a><span>5</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.nca0yzv523b"></a><span>Eco R</span></p></td><td class="c39"><p class="c4 c2"><a name="h.asxo1syv4hg1"></a><span>1</span></p></td><td class="c63"><p class="c4 c2"><a name="h.x71ph9ps5jlo"></a><span>0</span></p></td><td class="c90"><p class="c4 c2"><a name="h.caep0rvgrvh0"></a><span>0</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.5ago9jf3relr"></a><span>PstI</span></p></td><td class="c39"><p class="c4 c2"><a name="h.xxnp9vpfcyzi"></a><span>0</span></p></td><td class="c63"><p class="c4 c2"><a name="h.uh2ny991oqia"></a><span>0</span></p></td><td class="c90"><p class="c4 c2"><a name="h.ju4aq5ij8k44"></a><span>1</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.tqxwofs4qxbc"></a><span>XbaI</span></p></td><td class="c39"><p class="c4 c2"><a name="h.qihmsot6pl2a"></a><span>0</span></p></td><td class="c63"><p class="c4 c2"><a name="h.xtzo6ikfe7fq"></a><span>1</span></p></td><td class="c90"><p class="c4 c2"><a name="h.fje9ztr5fsb6"></a><span>1</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.pznia856efr"></a><span>SpcI</span></p></td><td class="c39"><p class="c4 c2"><a name="h.nxfafdazif22"></a><span>1</span></p></td><td class="c63"><p class="c4 c2"><a name="h.la1z3c7emzmo"></a><span>1</span></p></td><td class="c90"><p class="c4 c2"><a name="h.qceayaqmo7q1"></a><span>0</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.wnwxgm43end6"></a><span>Water</span></p></td><td class="c39"><p class="c4 c2"><a name="h.8ql6x1j0gvnk"></a><span>13</span></p></td><td class="c63"><p class="c4 c2"><a name="h.t9bpwap1kgw7"></a><span>13</span></p></td><td class="c90"><p class="c4 c2"><a name="h.7w9zite8hug9"></a><span>13</span></p></td></tr><tr class="c8"><td class="c52"><p class="c4 c2"><a name="h.pvrd163duddt"></a><span>Total</span></p></td><td class="c39"><p class="c4 c2"><a name="h.659ckxaagez4"></a><span>50</span></p></td><td class="c63"><p class="c4 c2"><a name="h.ig2gsn60ly02"></a><span>50</span></p></td><td class="c90"><p class="c4 c2"><a name="h.ieswbupikysh"></a><span>50</span></p></td></tr></tbody></table><p class="c1"><a name="h.wju7912zcsbi"></a></p><p class="c2"><a name="h.db9jjmra7drh"></a><span>8/6</span></p><p class="c2"><a name="h.xq6o2p8t3ekh"></a><span>Constitutive promoters</span></p><p class="c1"><a name="h.htlcv52tnfpc"></a></p><p class="c1"><a name="h.l6ban1o0huur"></a></p><a href="#" name="a706356d42cc6cb5710c29f11bea6cdecfddc9c2"></a><a href="#" name="25"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c95"><p class="c4 c2"><a name="h.wibnsic2a910"></a><span>CP Family member (strong)</span></p></td><td class="c98"><p class="c4 c2"><a name="h.6858tthab3pw"></a><span>BBa_J23119</span></p></td><td class="c89"><p class="c4 c2"><a name="h.x1z97dxzq6g7"></a><span>pSBIC3</span></p></td><td class="c17"><p class="c4 c2"><a name="h.gvhoulh69qzn"></a><span>2013 Kit plate 3</span></p></td><td class="c105"><p class="c4 c2"><a name="h.apwq70ggbn27"></a><span>18O</span></p></td><td class="c106"><p class="c4 c2"><a name="h.ints0anm9vhk"></a><span>i5-24-1</span></p></td></tr><tr class="c8"><td class="c95"><p class="c4 c2"><a name="h.t0ps5t1x8q4l"></a><span>CP Family member (medium)</span></p></td><td class="c98"><p class="c4 c2"><a name="h.v56droy9k0d8"></a><span>BBa_K823014 (J23118)</span></p></td><td class="c89"><p class="c4 c2"><a name="h.82leoibdgaqw"></a><span>pSBIC3</span></p></td><td class="c17"><p class="c4 c2"><a name="h.ezuwrzapwbbw"></a><span>2013 Kit plate 1</span></p></td><td class="c105"><p class="c4 c2"><a name="h.3lqdqk898cbd"></a><span>22M</span></p></td><td class="c106"><p class="c4 c2"><a name="h.pek0wjix7k64"></a><span>i5-24-2</span></p></td></tr><tr class="c8"><td class="c95"><p class="c4 c2"><a name="h.s5n956m48pp8"></a><span>CP Family member (weak)</span></p></td><td class="c98"><p class="c4 c2"><a name="h.3kc3i06t27zb"></a><span>BBa_K823012 (J23115)</span></p></td><td class="c89"><p class="c4 c2"><a name="h.jybz3h61sge6"></a><span>pSBIC3</span></p></td><td class="c17"><p class="c4 c2"><a name="h.h8fi7ukqrq2b"></a><span>2013 Kit plate 1</span></p></td><td class="c105"><p class="c4 c2"><a name="h.i92a4rin1bl2"></a><span>22I</span></p></td><td class="c106"><p class="c4 c2"><a name="h.o5q9bg9q4syf"></a><span>i5-24-3</span></p></td></tr></tbody></table><p class="c1"><a name="h.1ncejgt5yklj"></a></p><p class="c2"><a name="h.rycve1ih3gur"></a><span>Transformation </span></p><p class="c2"><a name="h.3jrw5j6p2sgm"></a><span>+ control i5-10-4 → 3 colonies </span></p><p class="c2"><a name="h.adynbsnxormw"></a><span>        i5-24-1 → 0 colonies</span></p><p class="c2"><a name="h.5rytshnqlc72"></a><span>        i5-24-2 → 25 colonies </span></p><p class="c2"><a name="h.k7raf6akjbw7"></a><span>        i5-24-3 → 15+ colonies</span></p><p class="c1"><a name="h.ixvvvk6gweyj"></a></p><p class="c2"><a name="h.5tcllztdk398"></a><span>Gel Extraction </span></p><p class="c1"><a name="h.vfrtwfc8f8cd"></a></p><a href="#" name="3b9b104010f4b2e1060d38a2f95b5f5fd7fba671"></a><a href="#" name="26"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c50"><p class="c1 c4"><a name="h.m0esxbrsc1wj"></a></p></td><td class="c50"><p class="c4 c2"><a name="h.i9bylpzyxh2"></a><span>Concentration (ng/ul)</span></p></td></tr><tr class="c8"><td class="c50"><p class="c4 c2"><a name="h.ints0anm9vhk"></a><span>i5-24-1 Com D</span></p></td><td class="c50"><p class="c4 c2"><a name="h.szs7o03b7gdz"></a><span>17.4</span></p></td></tr><tr class="c8"><td class="c50"><p class="c4 c2"><a name="h.pek0wjix7k64"></a><span>i5-24-2 Com E</span></p></td><td class="c50"><p class="c4 c2"><a name="h.ecp3xchccta6"></a><span>7.6</span></p></td></tr><tr class="c8"><td class="c50"><p class="c4 c2"><a name="h.o5q9bg9q4syf"></a><span>i5-24-3 ComA</span></p></td><td class="c50"><p class="c4 c2"><a name="h.37benhd42xmk"></a><span>7.9</span></p></td></tr><tr class="c8"><td class="c50"><p class="c4 c2"><a name="h.ints0anm9vhk"></a><span>i5-24-4 BackA</span></p></td><td class="c50"><p class="c4 c2"><a name="h.ia9g6gxge9v6"></a><span>13.3</span></p></td></tr><tr class="c8"><td class="c50"><p class="c4 c2"><a name="h.pek0wjix7k64"></a><span>i5-24-5 BackD</span></p></td><td class="c50"><p class="c4 c2"><a name="h.icjpxuqk2dxn"></a><span>11.6</span></p></td></tr><tr class="c8"><td class="c50"><p class="c4 c2"><a name="h.o5q9bg9q4syf"></a><span>i5-24-6 BackE</span></p></td><td class="c50"><p class="c4 c2"><a name="h.x6jb2cbnabnt"></a><span>9.6</span></p></td></tr></tbody></table><p class="c1"><a name="h.a0wn8d1nez2l"></a></p><p class="c2"><a name="h.vh0iwdhh5ns7"></a><span>8/7 </span></p><p class="c2"><a name="h.o7ul5ljt2ywf"></a><span>Inoculate from transformation 8/6 </span></p><p class="c2"><a name="h.2mgh77d71y1j"></a><span>i5-24-2 → 25+ colonies → picked 1 colony → i5-25-7</span></p><p class="c2"><a name="h.62ewmx28f7mt"></a><span>i5-24-3 → 15+ colonies → picked 1 colony → i5-25-8</span></p><p class="c1"><a name="h.yyhxqe7dj6zu"></a></p><p class="c2"><a name="h.iqqfoukq2oz"></a><span>Com D vector backbone dephosphorilation</span></p><p class="c2"><a name="h.h8c9smgqz13j"></a><span>i5-25-5 → i5-25-9 12.5 ng/ul</span></p><p class="c1"><a name="h.hmejr6cbbi1s"></a></p><p class="c2"><a name="h.8qibq2rdsosv"></a><span>Ligation </span></p><p class="c1"><a name="h.cr42wd9mm2t5"></a></p><a href="#" name="92d3062c8ee6a88c6fc20b0ac66d4fb45db13dbe"></a><a href="#" name="27"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c13"><p class="c1 c4"><a name="h.h7dmtux69b0h"></a></p></td><td class="c13"><p class="c4 c2"><a name="h.75fcnjetnhp0"></a><span>Com A 3:1 </span></p></td><td class="c13"><p class="c4 c2"><a name="h.ukmleeioa352"></a><span>Com A 0:1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.g3ghsm5hssqk"></a><span>Com D 3:1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.dnh0x7ywufq7"></a><span>Com D 0:1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.tg789oqh35vb"></a><span>Com E 3:1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.932dpd75v4u"></a><span>Com E 0:1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.u5a3yt7rngho"></a><span>Insert </span></p></td><td class="c13"><p class="c4 c2"><a name="h.bj9gpbdmzw02"></a><span>10</span></p></td><td class="c13"><p class="c4 c2"><a name="h.u3k3id4m92o3"></a><span>0</span></p></td><td class="c13"><p class="c4 c2"><a name="h.7upxcedqi2ni"></a><span>6</span></p></td><td class="c13"><p class="c4 c2"><a name="h.ag8ouhvjt9pj"></a><span>0</span></p></td><td class="c13"><p class="c4 c2"><a name="h.xu0iinttgao0"></a><span>8</span></p></td><td class="c13"><p class="c4 c2"><a name="h.wavjmvuq0zyn"></a><span>0</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.a484e1vh4vez"></a><span>Vector</span></p></td><td class="c13"><p class="c4 c2"><a name="h.x5hvz11tyyru"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.23wofbg7dk9o"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.so5pbs3hicb"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.3mxqleb9s8sn"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.6zldclloa19r"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.kgkhhly07n67"></a><span>5</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.fxjmuca5mbqo"></a><span>Buffer</span></p></td><td class="c13"><p class="c4 c2"><a name="h.684nr6u0aavk"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.f29ne6k35ks0"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.gwt9yiywu8g9"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.9p1fp38hzmrf"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.uw0txhnqieyp"></a><span>2</span></p></td><td class="c13"><p class="c4 c2"><a name="h.blp0k1q8ca3r"></a><span>2</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.eh8s8aai6ccl"></a><span>Ligase</span></p></td><td class="c13"><p class="c4 c2"><a name="h.rdx2fo9pjv50"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.afzclqfltvst"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.jn4ila6bgqp0"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.niyq33tmnvsd"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.mhdr3k2q70c4"></a><span>1</span></p></td><td class="c13"><p class="c4 c2"><a name="h.kdwsnfeco08e"></a><span>1</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.jhs7yvjkgipu"></a><span>Water</span></p></td><td class="c13"><p class="c4 c2"><a name="h.98uffvxft24t"></a><span>5</span></p></td><td class="c13"><p class="c4 c2"><a name="h.jx2mq28ndrhq"></a><span>15</span></p></td><td class="c13"><p class="c4 c2"><a name="h.avvmnx6rvcq7"></a><span>6</span></p></td><td class="c13"><p class="c4 c2"><a name="h.jomrcq1rkgq3"></a><span>12</span></p></td><td class="c13"><p class="c4 c2"><a name="h.2t6bgg8pq62k"></a><span>4</span></p></td><td class="c13"><p class="c4 c2"><a name="h.bk5em5zaqqk1"></a><span>12</span></p></td></tr><tr class="c8"><td class="c13"><p class="c4 c2"><a name="h.r4gakjxirnm"></a><span>Total</span></p></td><td class="c13"><p class="c4 c2"><a name="h.b7px5p629wb2"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.dr5wsphwugfr"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.1jukh4az3av"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.lv58iogydtel"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.rs9niqua7llp"></a><span>20</span></p></td><td class="c13"><p class="c4 c2"><a name="h.kjbbjk1msd6u"></a><span>20</span></p></td></tr></tbody></table><p class="c1"><a name="h.w7hero9cuyev"></a></p><p class="c2"><a name="h.2j6yxbeu2tab"></a><span>                insert → i5-23-3                        i5-25-1                                i5-25-2</span></p><p class="c2"><a name="h.3o8psi3w6gw"></a><span>                Vector → i5-25-4                        i5-25-9                                i5-25-6</span></p><p class="c2"><a name="h.5yfxj9jlbxfe"></a><span>                1                        2                3                4                5                6</span></p><p class="c1"><a name="h.p6m8jq20861m"></a></p><p class="c2"><a name="h.42bedh5ney9q"></a><span>Transformation </span></p><p class="c2"><a name="h.sn6yo85y7wf5"></a><span>i5-25-1 Com A 3:1 → 0 colonies </span></p><p class="c2"><a name="h.cl44wfisntbw"></a><span>i5-25-2 Com A 0:1 → 0 colonies</span></p><p class="c2"><a name="h.xgujaa4m9ze6"></a><span>i5-25-3 Com D 3:1 → 0 colonies</span></p><p class="c2"><a name="h.lu7vdyxwppfl"></a><span>i5-25-4 Com D 0:1 → 0 colonies </span></p><p class="c2"><a name="h.3kh89f44w1zf"></a><span>i5-25-9 Com E 3:1 →20 colonies → picked 3 colonies → i5-25-10</span></p><p class="c2"><a name="h.iehtfwff6p7f"></a><span>                                                → i5-25-11</span></p><p class="c2"><a name="h.5sydh01237my"></a><span>                                                → - control i5-25-12</span></p><p class="c2"><a name="h.vdvi3f1vqpef"></a><span>i5-25-6 Com E 0:1 → 0 colonies </span></p><p class="c2"><a name="h.7q6omna9ogct"></a><span>i5-11-5 + colonies → plenty colonies </span></p><p class="c1"><a name="h.zcyit1h53l3v"></a></p><p class="c2"><a name="h.86c253es1cm9"></a><span>8/8 </span></p><p class="c2"><a name="h.maa5grwkkatz"></a><span>Made glycerol stock from i5-15-7 and i5-25-8</span></p><p class="c2"><a name="h.ck7tfh7i7pmt"></a><span>Miniprep</span></p><a href="#" name="ec89fb10a3a2f50f8dc02e3b45423d2b3675faa4"></a><a href="#" name="28"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c93"><p class="c1 c4"><a name="h.3jpbcjkctjf6"></a></p></td><td class="c7"><p class="c4 c2"><a name="h.qs5drvo28rm7"></a><span>Concentration (ng/ul)</span></p></td></tr><tr class="c8"><td class="c93"><p class="c4 c2"><a name="h.t0j5ex3dratj"></a><span>i5-25-7 (medium CP)</span></p></td><td class="c7"><p class="c4 c2"><a name="h.91gep83p0uur"></a><span>109.3</span></p></td></tr><tr class="c8"><td class="c93"><p class="c4 c2"><a name="h.cutho5fp38n3"></a><span>i5-25-8 (small CP)</span></p></td><td class="c7"><p class="c4 c2"><a name="h.kxapg9r7ojwl"></a><span>116.7</span></p></td></tr></tbody></table><p class="c1"><a name="h.xtxmx6x6whny"></a></p><p class="c2"><a name="h.bvnzeytbhsfd"></a><span>Transformation</span></p><p class="c2"><a name="h.wg6vhgd3o65d"></a><span>i5-26-1        ComA 3:1</span></p><p class="c2"><a name="h.h76705w0uaeb"></a><span>i5-26-2        ComD 3:1</span></p><p class="c2"><a name="h.r92f30awsl43"></a><span>i5-26-3 pLac-GFP (+ control) </span></p><p class="c2"><a name="h.3dlfciq4c227"></a><span>i5-26-4 ComE 3:1</span></p><p class="c1"><a name="h.aj4foswu0mmv"></a></p><p class="c2"><a name="h.s3iv9m4193k7"></a><span>Digestion </span></p><p class="c1"><a name="h.sa3hn2besxn6"></a></p><a href="#" name="0f6777305afa90a7628766949228ff86f8e7762f"></a><a href="#" name="29"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c3"><p class="c1 c4"><a name="h.1ybxzsmmqky1"></a></p></td><td class="c3"><p class="c4 c2"><a name="h.cztt9ea1hy78"></a><span>i5-26-4</span></p></td><td class="c3"><p class="c4 c2"><a name="h.jxlyl7ugm2hk"></a><span>i5-26-5</span></p></td><td class="c3"><p class="c4 c2"><a name="h.12pcqlm112un"></a><span>i5-26-6</span></p></td><td class="c3"><p class="c4 c2"><a name="h.wk9jf1mpv9w8"></a><span>i5-26-7</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.evm95gi6jtb1"></a><span>DNA</span></p></td><td class="c3"><p class="c4 c2"><a name="h.xzp8jpql5w90"></a><span>30</span></p></td><td class="c3"><p class="c4 c2"><a name="h.43vqez2vhz75"></a><span>11</span></p></td><td class="c3"><p class="c4 c2"><a name="h.vsidxwd6b7mg"></a><span>20</span></p></td><td class="c3"><p class="c4 c2"><a name="h.yxlmuzunsphy"></a><span>20</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.11w80jnnzisx"></a><span>Buffer</span></p></td><td class="c3"><p class="c4 c2"><a name="h.op2rau3vwoi1"></a><span>5</span></p></td><td class="c3"><p class="c4 c2"><a name="h.u5r2g4aik22j"></a><span>5</span></p></td><td class="c3"><p class="c4 c2"><a name="h.93b535wzow5k"></a><span>5</span></p></td><td class="c3"><p class="c4 c2"><a name="h.2ohgeht0b18a"></a><span>5</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.48w8qfo4euj6"></a><span>PstI</span></p></td><td class="c3"><p class="c4 c2"><a name="h.5pgl0skigbuo"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.r168jkjs4pk"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.4djrpkwjmoyu"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.cdxwrql0am71"></a><span>1</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.h6zo64dmnvbu"></a><span>SpcI</span></p></td><td class="c3"><p class="c4 c2"><a name="h.pedgoamanh83"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.bano6kt8m2br"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.df63q63ruwu3"></a><span>1</span></p></td><td class="c3"><p class="c4 c2"><a name="h.4rgl5cj93v1p"></a><span>1</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.veh91msc06en"></a><span>Water</span></p></td><td class="c3"><p class="c4 c2"><a name="h.damc93mc41p6"></a><span>13</span></p></td><td class="c3"><p class="c4 c2"><a name="h.4ss3yymvo82g"></a><span>32</span></p></td><td class="c3"><p class="c4 c2"><a name="h.7uzwotz35pb0"></a><span>23</span></p></td><td class="c3"><p class="c4 c2"><a name="h.2njg98ojeu4w"></a><span>23</span></p></td></tr><tr class="c8"><td class="c3"><p class="c4 c2"><a name="h.iwuuy5rg7fcj"></a><span>Total</span></p></td><td class="c3"><p class="c4 c2"><a name="h.82wh9ptmr0k7"></a><span>50</span></p></td><td class="c3"><p class="c4 c2"><a name="h.lzlefu8t5gkh"></a><span>50</span></p></td><td class="c3"><p class="c4 c2"><a name="h.qy4uj2gg7gii"></a><span>50</span></p></td><td class="c3"><p class="c4 c2"><a name="h.6fj46w7i9aw9"></a><span>50</span></p></td></tr></tbody></table><p class="c1"><a name="h.q7os7wcvo5yl"></a></p><p class="c1"><a name="h.8oucsdmb4l50"></a></p><a href="#" name="ef197295390ef46b61c6f71de9807a81f1f2109a"></a><a href="#" name="30"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c42"><p class="c1 c4"><a name="h.7ohb181cvlc5"></a></p></td><td class="c42"><p class="c4 c2"><a name="h.1ndnlsrexn6p"></a><span>i5-26-8</span></p></td><td class="c42"><p class="c4 c2"><a name="h.yvwbj81t7251"></a><span>i5-26-9</span></p></td><td class="c42"><p class="c4 c2"><a name="h.4z2y4vcanl7x"></a><span>i5-26-10</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.bb6yew8hwcb5"></a><span>DNA</span></p></td><td class="c42"><p class="c4 c2"><a name="h.tcf5ai7nvoqi"></a><span>20</span></p></td><td class="c42"><p class="c4 c2"><a name="h.30ln3nwv5bi0"></a><span>20</span></p></td><td class="c42"><p class="c4 c2"><a name="h.dbwm952yixl0"></a><span>20</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.u3c5408277v5"></a><span>Buffer</span></p></td><td class="c42"><p class="c4 c2"><a name="h.4k8p8j95ks0u"></a><span>5</span></p></td><td class="c42"><p class="c4 c2"><a name="h.asyhjt5797kh"></a><span>5</span></p></td><td class="c42"><p class="c4 c2"><a name="h.u0pcfq9fffqb"></a><span>5</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.tqq52md11g1r"></a><span>PstI</span></p></td><td class="c42"><p class="c4 c2"><a name="h.gqx5h1gwtiph"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.8gr973v6w2ij"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.j8dbj5ybpdft"></a><span>1</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.o2auivle7b2"></a><span>XbaI</span></p></td><td class="c42"><p class="c4 c2"><a name="h.s65sqjtdl1fv"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.de99c0rajslu"></a><span>1</span></p></td><td class="c42"><p class="c4 c2"><a name="h.gso7obnhep95"></a><span>1</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.aop280y95qcn"></a><span>Water</span></p></td><td class="c42"><p class="c4 c2"><a name="h.bsebvjwtdzro"></a><span>23</span></p></td><td class="c42"><p class="c4 c2"><a name="h.o5xzj7daaev0"></a><span>23</span></p></td><td class="c42"><p class="c4 c2"><a name="h.2raz43srotx"></a><span>23</span></p></td></tr><tr class="c8"><td class="c42"><p class="c4 c2"><a name="h.xy9f3ac7jb3n"></a><span>Total</span></p></td><td class="c42"><p class="c4 c2"><a name="h.z39vfuuiv370"></a><span>50</span></p></td><td class="c42"><p class="c4 c2"><a name="h.ce8zmsqe95xj"></a><span>50</span></p></td><td class="c42"><p class="c4 c2"><a name="h.6p64omi9z28m"></a><span>50</span></p></td></tr></tbody></table><p class="c1"><a name="h.kamtocqtkydk"></a></p><p class="c2"><a name="h.cpb6f9oy218h"></a><span>Transformation </span></p><p class="c2"><a name="h.82l5yurfhh46"></a><span>3:1 ComA</span></p><p class="c2"><a name="h.zc9adneqyswj"></a><span>3:1 ComD</span></p><p class="c2"><a name="h.u4z7e8tb0rrs"></a><span>3:1 ComE</span></p><p class="c2"><a name="h.ib77a1l87t48"></a><span>+ control (pLac-GFP)</span></p><p class="c1"><a name="h.eh7ds6f1gnpm"></a></p><p class="c2"><a name="h.f00tibgergp"></a><span>8/9</span></p><p class="c2"><a name="h.kodcs5othrdp"></a><span>Ligation </span></p><p class="c2"><a name="h.z0kwt5nxopux"></a><span>insert: i5-26-10 Vector i5-26-5</span></p><p class="c1"><a name="h.9h5o97z62ydu"></a></p><a href="#" name="19791e3fc694b2233e965d69ebe788cf41afb465"></a><a href="#" name="31"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c16"><p class="c1 c4"><a name="h.7vluc54wk01l"></a></p></td><td class="c57"><p class="c4 c2"><a name="h.k6mui7b6rz68"></a><span>3:1</span></p></td><td class="c43"><p class="c4 c2"><a name="h.dgvrvsc7bu0g"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c16"><p class="c4 c2"><a name="h.oub71i2pw3n2"></a><span>Insert</span></p></td><td class="c57"><p class="c4 c2"><a name="h.amz5pvvj9q3t"></a><span>6.3</span></p></td><td class="c43"><p class="c4 c2"><a name="h.g3hp3ac60ptf"></a><span>0</span></p></td></tr><tr class="c8"><td class="c16"><p class="c4 c2"><a name="h.ba6duxxfy39o"></a><span>Vector</span></p></td><td class="c57"><p class="c4 c2"><a name="h.60oo94a93h0b"></a><span>2</span></p></td><td class="c43"><p class="c4 c2"><a name="h.n9l5xk276hqv"></a><span>2</span></p></td></tr><tr class="c8"><td class="c16"><p class="c4 c2"><a name="h.yly4vsjehj4"></a><span>Buffer</span></p></td><td class="c57"><p class="c4 c2"><a name="h.m9ba0nwqm5pd"></a><span>2</span></p></td><td class="c43"><p class="c4 c2"><a name="h.z71k2r76mt7f"></a><span>2</span></p></td></tr><tr class="c8"><td class="c16"><p class="c4 c2"><a name="h.abw7saw8owjz"></a><span>Ligase</span></p></td><td class="c57"><p class="c4 c2"><a name="h.q3wgcgmtgmn4"></a><span>1</span></p></td><td class="c43"><p class="c4 c2"><a name="h.6dxg7vck2gbh"></a><span>1</span></p></td></tr><tr class="c8"><td class="c16"><p class="c4 c2"><a name="h.erqt6icosda0"></a><span>Water</span></p></td><td class="c57"><p class="c4 c2"><a name="h.jl1ceixsbhx7"></a><span>8.7</span></p></td><td class="c43"><p class="c4 c2"><a name="h.gfwp6asmt3ku"></a><span>15</span></p></td></tr><tr class="c8"><td class="c16"><p class="c4 c2"><a name="h.3ufubvxg739z"></a><span>Total</span></p></td><td class="c57"><p class="c4 c2"><a name="h.l6j7c6inlwdf"></a><span>20</span></p></td><td class="c43"><p class="c4 c2"><a name="h.zdtgvwkzd1kj"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.68jcggnxe2kk"></a><span>                        1        2</span></p><p class="c1"><a name="h.xbyphphtdb9x"></a></p><p class="c2"><a name="h.13k2y8znsdx"></a><span>Insert i5-26-10 Vector i5-25-4</span></p><p class="c1"><a name="h.1pw16fkk8vpm"></a></p><a href="#" name="d17a5321e97ae3ad32f7b3ff7218a6be7531f799"></a><a href="#" name="32"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c33"><p class="c1 c4"><a name="h.peun7r6p3on6"></a></p></td><td class="c54"><p class="c4 c2"><a name="h.asit7cs2rk80"></a><span>3:1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.xgvt5agishz"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c33"><p class="c4 c2"><a name="h.oub71i2pw3n2"></a><span>Insert</span></p></td><td class="c54"><p class="c4 c2"><a name="h.xt55nt6qk32q"></a><span>6.6</span></p></td><td class="c11"><p class="c4 c2"><a name="h.3vknl45azwvf"></a><span>0</span></p></td></tr><tr class="c8"><td class="c33"><p class="c4 c2"><a name="h.ba6duxxfy39o"></a><span>Vector</span></p></td><td class="c54"><p class="c4 c2"><a name="h.d392mwaeapid"></a><span>2.5</span></p></td><td class="c11"><p class="c4 c2"><a name="h.vt7xt8wbfrnz"></a><span>2.5</span></p></td></tr><tr class="c8"><td class="c33"><p class="c4 c2"><a name="h.yly4vsjehj4"></a><span>Buffer</span></p></td><td class="c54"><p class="c4 c2"><a name="h.8gz1siehn7ls"></a><span>2</span></p></td><td class="c11"><p class="c4 c2"><a name="h.m5w9b5bldgpf"></a><span>2</span></p></td></tr><tr class="c8"><td class="c33"><p class="c4 c2"><a name="h.abw7saw8owjz"></a><span>Ligase</span></p></td><td class="c54"><p class="c4 c2"><a name="h.7dv0ve66io28"></a><span>1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.e8rwfes5l0e6"></a><span>1</span></p></td></tr><tr class="c8"><td class="c33"><p class="c4 c2"><a name="h.erqt6icosda0"></a><span>Water</span></p></td><td class="c54"><p class="c4 c2"><a name="h.ygv2qmektbul"></a><span>7.3</span></p></td><td class="c11"><p class="c4 c2"><a name="h.nwq9epaajli6"></a><span>14.5</span></p></td></tr><tr class="c8"><td class="c33"><p class="c4 c2"><a name="h.3ufubvxg739z"></a><span>Total</span></p></td><td class="c54"><p class="c4 c2"><a name="h.k4b8pbveipsm"></a><span>20</span></p></td><td class="c11"><p class="c4 c2"><a name="h.9a8kvehi89fe"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.ssbodvtory9t"></a><span>                3                4</span></p><p class="c1"><a name="h.ml1j8fbaxfv8"></a></p><p class="c2"><a name="h.l4rs6e81pgwk"></a><span>Insert i5-26-8  Vector i5-26-6</span></p><p class="c1"><a name="h.4xblm2trv2u8"></a></p><a href="#" name="aaf417d0d211b075e9abdd58751a67903bdd32fa"></a><a href="#" name="33"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c58"><p class="c1 c4"><a name="h.o14kiavtq1a6"></a></p></td><td class="c35"><p class="c4 c2"><a name="h.2p4wl2lj83k8"></a><span>3:1</span></p></td><td class="c53"><p class="c4 c2"><a name="h.1s8hl78y6siy"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c58"><p class="c4 c2"><a name="h.oub71i2pw3n2"></a><span>Insert</span></p></td><td class="c35"><p class="c4 c2"><a name="h.rsxvxg7ie1av"></a><span>4.36</span></p></td><td class="c53"><p class="c4 c2"><a name="h.q6l6h93wnemx"></a><span>0</span></p></td></tr><tr class="c8"><td class="c58"><p class="c4 c2"><a name="h.ba6duxxfy39o"></a><span>Vector</span></p></td><td class="c35"><p class="c4 c2"><a name="h.xhwradv7ofd0"></a><span>2</span></p></td><td class="c53"><p class="c4 c2"><a name="h.hqbqsoydztdo"></a><span>2</span></p></td></tr><tr class="c8"><td class="c58"><p class="c4 c2"><a name="h.yly4vsjehj4"></a><span>Buffer</span></p></td><td class="c35"><p class="c4 c2"><a name="h.5uydunqvx82s"></a><span>2</span></p></td><td class="c53"><p class="c4 c2"><a name="h.lg03ux2ir8a3"></a><span>2</span></p></td></tr><tr class="c8"><td class="c58"><p class="c4 c2"><a name="h.abw7saw8owjz"></a><span>Ligase</span></p></td><td class="c35"><p class="c4 c2"><a name="h.963w6glbch7w"></a><span>1</span></p></td><td class="c53"><p class="c4 c2"><a name="h.avgzsiyct428"></a><span>1</span></p></td></tr><tr class="c8"><td class="c58"><p class="c4 c2"><a name="h.erqt6icosda0"></a><span>Water</span></p></td><td class="c35"><p class="c4 c2"><a name="h.mn8l1ghufu4q"></a><span>10.64</span></p></td><td class="c53"><p class="c4 c2"><a name="h.fip39ewf67qm"></a><span>15</span></p></td></tr><tr class="c8"><td class="c58"><p class="c4 c2"><a name="h.3ufubvxg739z"></a><span>Total</span></p></td><td class="c35"><p class="c4 c2"><a name="h.oz0oalujao1s"></a><span>20</span></p></td><td class="c53"><p class="c4 c2"><a name="h.qt6t1rqi4nbf"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.cewwqq7q8bj1"></a><span>                5                6</span></p><p class="c2"><a name="h.4dvgy01tjgh8"></a><span>insert i5-26-9 Vector i5-26-6</span></p><p class="c1"><a name="h.dlzdlm52ue9"></a></p><a href="#" name="d04ab804a74f9748d8914ea8ae7ab6df1f55f82f"></a><a href="#" name="34"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c84"><p class="c1 c4"><a name="h.uk8xljkz43q0"></a></p></td><td class="c14"><p class="c4 c2"><a name="h.rm2a91ypwctm"></a><span>3:1</span></p></td><td class="c44"><p class="c4 c2"><a name="h.rlch06w846tp"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c84"><p class="c4 c2"><a name="h.u924bruxam85"></a><span>insert</span></p></td><td class="c14"><p class="c4 c2"><a name="h.2bhmpi28qiop"></a><span>2.3</span></p></td><td class="c44"><p class="c4 c2"><a name="h.v61xzojcboi6"></a><span>0</span></p></td></tr><tr class="c8"><td class="c84"><p class="c4 c2"><a name="h.fdocnt4y7ji3"></a><span>vector</span></p></td><td class="c14"><p class="c4 c2"><a name="h.sjk306r40m4u"></a><span>2</span></p></td><td class="c44"><p class="c4 c2"><a name="h.se475ae81qyn"></a><span>2</span></p></td></tr><tr class="c8"><td class="c84"><p class="c4 c2"><a name="h.t4a7k41jiwj"></a><span>buffer</span></p></td><td class="c14"><p class="c4 c2"><a name="h.o3qtri6whr8e"></a><span>2</span></p></td><td class="c44"><p class="c4 c2"><a name="h.e5p264vlll3s"></a><span>2</span></p></td></tr><tr class="c8"><td class="c84"><p class="c4 c2"><a name="h.7cppxveawh2b"></a><span>ligase</span></p></td><td class="c14"><p class="c4 c2"><a name="h.a29fc4w34toq"></a><span>1</span></p></td><td class="c44"><p class="c4 c2"><a name="h.e99nn4f6ts9w"></a><span>1</span></p></td></tr><tr class="c8"><td class="c84"><p class="c4 c2"><a name="h.ob97c8cj4okf"></a><span>water</span></p></td><td class="c14"><p class="c4 c2"><a name="h.ze8koa8orksh"></a><span>12.7</span></p></td><td class="c44"><p class="c4 c2"><a name="h.f77rwsefozr5"></a><span>15</span></p></td></tr><tr class="c8"><td class="c84"><p class="c4 c2"><a name="h.1qxb72m36rfj"></a><span>total</span></p></td><td class="c14"><p class="c4 c2"><a name="h.8b6ffl6k30mh"></a><span>20</span></p></td><td class="c44"><p class="c4 c2"><a name="h.chvq5gaw562h"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.5h8su0c8cvoe"></a><span>                7                8</span></p><p class="c1"><a name="h.3g8l51vx5mmt"></a></p><p class="c2"><a name="h.31elyj5r49k7"></a><span>Transformation</span></p><p class="c2"><a name="h.v1z8vn6xu3il"></a><span>Control (w/ GFP) [i5-10-1] → i5-28-1</span></p><p class="c2"><a name="h.qakg30ck28x3"></a><span>Control (w/out GFP) [i5-22-2] → i5-28-2</span></p><p class="c1"><a name="h.9d158dy3dj5z"></a></p><p class="c2"><a name="h.tz9j1wbg7mr3"></a><span>1 → i5-28-3</span></p><p class="c2"><a name="h.8il6mtefdzrt"></a><span>2 → i5-28-4</span></p><p class="c2"><a name="h.747dg3btie74"></a><span>3 → i5-28-5</span></p><p class="c2"><a name="h.9540h4uswcxa"></a><span>4 → i5-28-6        no growth </span></p><p class="c2"><a name="h.lrkw3udcpxuo"></a><span>5 → i5-28-7</span></p><p class="c2"><a name="h.58jyy6t51cy7"></a><span>6 → i5-28-8</span></p><p class="c2"><a name="h.vwu0q7nzmq1k"></a><span>7→ i5-28-9</span></p><p class="c2"><a name="h.2zsv5y78rfix"></a><span>8 → i5-28-10 </span></p><p class="c1"><a name="h.hbt714lmlqv9"></a></p><p class="c2"><a name="h.73fhqn2jb5kj"></a><span>Ligation</span></p><p class="c2"><a name="h.g3cakqv1n2j"></a><span>redo from 8/7</span></p><p class="c1"><a name="h.213inz40gxm2"></a></p><p class="c2"><a name="h.5qyjxe42jdd2"></a><span>Transformation </span></p><p class="c2"><a name="h.te5asa59w3uc"></a><span>ComA 3:1</span></p><p class="c2"><a name="h.49y1cm5hrwwa"></a><span>Com A 0:1</span></p><p class="c2"><a name="h.4m8d55jvn825"></a><span>Com D 3:1</span></p><p class="c2"><a name="h.4afpflrq2tlb"></a><span>Com D 0:1</span></p><p class="c2"><a name="h.6s97dwn6g7av"></a><span>ComE 3:1</span></p><p class="c2"><a name="h.euuof7rbjt5n"></a><span>Com E 0:1</span></p><p class="c2"><a name="h.1e00fxu6rigc"></a><span>pLac-GFP as + control</span></p><p class="c1"><a name="h.r9jiwbxkqjp"></a></p><p class="c2"><a name="h.8asdz9ocehq7"></a><span>8/10 </span></p><p class="c2"><a name="h.9tu8znned25x"></a><span>ligation </span></p><p class="c2"><a name="h.7ggfrz5jwixt"></a><span>insert: i5-26-10 Vector i5-26-5</span></p><p class="c1"><a name="h.fs330v6uzncj"></a></p><a href="#" name="8f02dfea07dc25a4d7910b4bcdabaf0828fd7502"></a><a href="#" name="35"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c60"><p class="c1 c4"><a name="h.lr1ucm3je5ch"></a></p></td><td class="c34"><p class="c4 c2"><a name="h.1tdfkaxf72hu"></a><span>3:1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.irgy751q45bb"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.29frdg7sf7qs"></a><span>insert</span></p></td><td class="c34"><p class="c4 c2"><a name="h.3jo10h4lfbwa"></a><span>6.3</span></p></td><td class="c11"><p class="c4 c2"><a name="h.66r7wdylz39h"></a><span>0</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.grelxwnto9yw"></a><span>vector</span></p></td><td class="c34"><p class="c4 c2"><a name="h.3uzirpqv6238"></a><span>2</span></p></td><td class="c11"><p class="c4 c2"><a name="h.f2tp69mzqtu8"></a><span>2</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.mr4s0tbd3zsx"></a><span>buffer</span></p></td><td class="c34"><p class="c4 c2"><a name="h.5d0nzx5y3dd7"></a><span>2</span></p></td><td class="c11"><p class="c4 c2"><a name="h.b7g51hyqpul1"></a><span>2</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.voe786qcl1rf"></a><span>ligase</span></p></td><td class="c34"><p class="c4 c2"><a name="h.q5ym06cr9hin"></a><span>1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.o7p5fhdl1qx2"></a><span>1</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.kvnjo4exssvp"></a><span>water</span></p></td><td class="c34"><p class="c4 c2"><a name="h.xaalwyd7nrzb"></a><span>8.7</span></p></td><td class="c11"><p class="c4 c2"><a name="h.8xb8uwpml5mk"></a><span>15</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.rrlxvuele5sw"></a><span>total</span></p></td><td class="c34"><p class="c4 c2"><a name="h.mwokkpi7pthu"></a><span>20</span></p></td><td class="c11"><p class="c4 c2"><a name="h.xke9jmf50d4i"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.mrcdf6e8qi1q"></a><span>                1                2</span></p><p class="c2"><a name="h.8pcb8d35m6as"></a><span>insert i5-26-10 vector i5-26-4</span></p><p class="c1"><a name="h.fs330v6uzncj"></a></p><a href="#" name="d9c4c682c5375fd0d45dd4d7f3730aaa47820b8a"></a><a href="#" name="36"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c60"><p class="c1 c4"><a name="h.lr1ucm3je5ch"></a></p></td><td class="c34"><p class="c4 c2"><a name="h.1tdfkaxf72hu"></a><span>3:1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.irgy751q45bb"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.29frdg7sf7qs"></a><span>insert</span></p></td><td class="c34"><p class="c4 c2"><a name="h.3jo10h4lfbwa"></a><span>6.6</span></p></td><td class="c11"><p class="c4 c2"><a name="h.66r7wdylz39h"></a><span>0</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.grelxwnto9yw"></a><span>vector</span></p></td><td class="c34"><p class="c4 c2"><a name="h.3uzirpqv6238"></a><span>2.5</span></p></td><td class="c11"><p class="c4 c2"><a name="h.f2tp69mzqtu8"></a><span>2.5</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.mr4s0tbd3zsx"></a><span>buffer</span></p></td><td class="c34"><p class="c4 c2"><a name="h.5d0nzx5y3dd7"></a><span>2</span></p></td><td class="c11"><p class="c4 c2"><a name="h.b7g51hyqpul1"></a><span>2</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.voe786qcl1rf"></a><span>ligase</span></p></td><td class="c34"><p class="c4 c2"><a name="h.q5ym06cr9hin"></a><span>1</span></p></td><td class="c11"><p class="c4 c2"><a name="h.o7p5fhdl1qx2"></a><span>1</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.kvnjo4exssvp"></a><span>water</span></p></td><td class="c34"><p class="c4 c2"><a name="h.xaalwyd7nrzb"></a><span>7.3</span></p></td><td class="c11"><p class="c4 c2"><a name="h.8xb8uwpml5mk"></a><span>14.5</span></p></td></tr><tr class="c8"><td class="c60"><p class="c4 c2"><a name="h.rrlxvuele5sw"></a><span>total</span></p></td><td class="c34"><p class="c4 c2"><a name="h.mwokkpi7pthu"></a><span>20</span></p></td><td class="c11"><p class="c4 c2"><a name="h.xke9jmf50d4i"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.2zzkjr266vom"></a><span>                3                4</span></p><p class="c2"><a name="h.c7ej88x5dknt"></a><span>insert: i5-26-8 vector: i5-26-6</span></p><p class="c1"><a name="h.75jan7t5a5wa"></a></p><a href="#" name="fb06182ab4eca39bc376798c31660a3ff39e3dbc"></a><a href="#" name="37"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c49"><p class="c1 c4"><a name="h.rp0xx4ndzdnc"></a></p></td><td class="c54"><p class="c4 c2"><a name="h.mcz4896ozeum"></a><span>3:1</span></p></td><td class="c59"><p class="c4 c2"><a name="h.vxgp54ojxuvj"></a><span>0:1</span></p></td></tr><tr class="c8"><td class="c49"><p class="c4 c2"><a name="h.qausojphcizg"></a><span>insert</span></p></td><td class="c54"><p class="c4 c2"><a name="h.9nynadi0m07"></a><span>4.4</span></p></td><td class="c59"><p class="c4 c2"><a name="h.tqh3z8gi5eem"></a><span>0</span></p></td></tr><tr class="c8"><td class="c49"><p class="c4 c2"><a name="h.g8daez7kpm6m"></a><span>vector</span></p></td><td class="c54"><p class="c4 c2"><a name="h.zbn5uiicfri2"></a><span>2</span></p></td><td class="c59"><p class="c4 c2"><a name="h.de9a00gs89ot"></a><span>2</span></p></td></tr><tr class="c8"><td class="c49"><p class="c4 c2"><a name="h.qc6mrhqcnlyr"></a><span>buffer</span></p></td><td class="c54"><p class="c4 c2"><a name="h.pcgj2fa8t2fk"></a><span>2</span></p></td><td class="c59"><p class="c4 c2"><a name="h.lz8uvgragy7d"></a><span>2</span></p></td></tr><tr class="c8"><td class="c49"><p class="c4 c2"><a name="h.nxwjrpkmzq6v"></a><span>ligase</span></p></td><td class="c54"><p class="c4 c2"><a name="h.atwez3jpqgqh"></a><span>1</span></p></td><td class="c59"><p class="c4 c2"><a name="h.knj4v3lna52d"></a><span>1</span></p></td></tr><tr class="c8"><td class="c49"><p class="c4 c2"><a name="h.3mc1x2tvomwu"></a><span>water</span></p></td><td class="c54"><p class="c4 c2"><a name="h.yuz1fyimsmsf"></a><span>10.6</span></p></td><td class="c59"><p class="c4 c2"><a name="h.6bzfezftr4lh"></a><span>15</span></p></td></tr><tr class="c8"><td class="c49"><p class="c4 c2"><a name="h.eqqsvyoz2beq"></a><span>total</span></p></td><td class="c54"><p class="c4 c2"><a name="h.slsnq3q69jdh"></a><span>20</span></p></td><td class="c59"><p class="c4 c2"><a name="h.za6kz0jioq5y"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.5jjln8h6k8kb"></a><span>                        5                6</span></p><p class="c2"><a name="h.y7etqamfqm6a"></a><span>insert i5-26-9 Vector i5-26-6</span></p><p class="c1"><a name="h.j08bvg6vg5b6"></a></p><a href="#" name="2b6ea62b0c59c28b37143d040b37dd7b568cb551"></a><a href="#" name="38"></a><table class="c12" cellpadding="0" cellspacing="0"><tbody><tr class="c8"><td class="c101"><p class="c1 c4"><a name="h.o9c2o0qsvdh"></a></p></td><td class="c59"><p class="c4 c2"><a name="h.xr45vdntahox"></a><span>3:1</span></p></td></tr><tr class="c8"><td class="c101"><p class="c4 c2"><a name="h.xg71pdalpo99"></a><span>insert</span></p></td><td class="c59"><p class="c4 c2"><a name="h.9i2exop4xi3i"></a><span>5.1</span></p></td></tr><tr class="c8"><td class="c101"><p class="c4 c2"><a name="h.tnj7o45aslz6"></a><span>vector</span></p></td><td class="c59"><p class="c4 c2"><a name="h.4jq6y5rz3ega"></a><span>2</span></p></td></tr><tr class="c8"><td class="c101"><p class="c4 c2"><a name="h.8rl0imxjpnln"></a><span>buffer</span></p></td><td class="c59"><p class="c4 c2"><a name="h.h7ae1fba1ius"></a><span>2</span></p></td></tr><tr class="c8"><td class="c101"><p class="c4 c2"><a name="h.3n097hgtubt9"></a><span>ligase</span></p></td><td class="c59"><p class="c4 c2"><a name="h.1h6286lbzxyx"></a><span>1</span></p></td></tr><tr class="c8"><td class="c101"><p class="c4 c2"><a name="h.6j9jobvb4735"></a><span>water</span></p></td><td class="c59"><p class="c4 c2"><a name="h.jdxhm6vm65zt"></a><span>9.9</span></p></td></tr><tr class="c8"><td class="c101"><p class="c4 c2"><a name="h.qf5w6k7j91ln"></a><span>total</span></p></td><td class="c59"><p class="c4 c2"><a name="h.7srltd35ouat"></a><span>20</span></p></td></tr></tbody></table><p class="c2"><a name="h.2oy2xb6en0p2"></a><span>                7</span></p><p class="c2"><a name="h.sio52xcr0lwf"></a><span>8/10</span></p><p class="c2"><a name="h.424r9tg71t6"></a><span>Transformation </span></p><p class="c2"><a name="h.qv53featg3ve"></a><span>1 → i5-30-1 → 5+ colonies [pFru-RBS-GFP]</span></p><p class="c2"><a name="h.2mrodfqf4cj5"></a><span>2 → i5-30-2 → 0 colonies</span></p><p class="c2"><a name="h.h1ailh1sd55k"></a><span>3 → i5-30-3 → 5 colonies f[pScr-RBS-GFP]</span></p><p class="c2"><a name="h.h9xnfa7sv8gj"></a><span>4 → i5-30-4→ 0 colonies </span></p><p class="c2"><a name="h.f5rdsf7n4y3l"></a><span>5 → i5-30-5 → 60+ colonies [CP-Cra]</span></p><p class="c2"><a name="h.64iburyxf127"></a><span>6 → i5-30-6 → 60+ colonies </span></p><p class="c2"><a name="h.bi19hrunzili"></a><span>7 → i5-30-7 → 60+ colonies  [CP-ScR]</span></p>