Template:Kyoto/Notebook/Sep 8

From 2013.igem.org

(Difference between revisions)
(→Ligation)
(→Gel Extraction)
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==Sep 8==
==Sep 8==
===Gel Extraction===
===Gel Extraction===
-
<!-- こっから -->
 
<div class="experiment">
<div class="experiment">
-
<span class="author">じっけんしゃ</span>
+
<span class="author">No name</span>
{| class="wikitable"
{| class="wikitable"
!Lane||DNA||Enzyme
!Lane||DNA||Enzyme
Line 9: Line 8:
|1||100bp ladder||
|1||100bp ladder||
|-
|-
-
|2||RBS-lysis1-DT||X+P
+
|2||RBS-lysis1-DT||XbaI & PstI
|-
|-
-
|3||RBS-lysis1-DT||X+P
+
|3||RBS-lysis1-DT||XbaI & PstI
|-
|-
-
|5||RBS-lysis3-DT||X+P
+
|5||RBS-lysis3-DT||XbaI & PstI
|-
|-
-
|6||RBS-lysis3-DT||X+P
+
|6||RBS-lysis3-DT||XbaI & PstI
|}
|}
[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxafterxx.xxx]]<br>
[[File:igku_xxafterxx.xxx]]<br>
{| class="wikitable"
{| class="wikitable"
-
!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
+
!Name||concentration[&micro;g/mL]||260/280||260/230
|-
|-
-
|RBS-lysis1-DT(X+P)|| ||5.3||1.98||0.06
+
|RBS-lysis1-DT (XbaI & PstI)||5.3||1.98||0.06
|-
|-
-
|RBS-lysis3-DT(X+P)|| ||5.3||1.98||0.06
+
|RBS-lysis3-DT (XbaI & PstI)||5.3||1.98||0.06
|}
|}
-
</div>
 
{| class="wikitable"
{| class="wikitable"
!Lane||DNA||Enzyme
!Lane||DNA||Enzyme
Line 32: Line 30:
|1||100bp ladder||
|1||100bp ladder||
|-
|-
-
|3||Pcon-pT181 atte||E+S
+
|3||Pcon-pT181 atte||EcoRI & SpeI
|-
|-
-
|4||Pcon-pT181 atte||E+S
+
|4||Pcon-pT181 atte||EcoRI & SpeI
|-
|-
-
|6||Spinach-DT||X+P
+
|6||Spinach-DT||XbaI & PstI
|-
|-
-
|7||Spinach-DT||X+P
+
|7||Spinach-DT||XbaI & PstI
|-
|-
-
|9||RBS-lacZα-DT||X+P
+
|9||RBS-lacZ&alpha;-DT||XbaI & PstI
|-
|-
-
|10||RBS-lacZα-DT||X+P
+
|10||RBS-lacZ&alpha;-DT||XbaI & PstI
|}
|}
[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxafterxx.xxx]]<br>
[[File:igku_xxafterxx.xxx]]<br>
{| class="wikitable"
{| class="wikitable"
-
!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
+
!Name||concentration[&micro;g/mL]||260/280||260/230
|-
|-
-
|Pcon-pT181 atte(E+S)|| ||5.2||1.82||0.36
+
|Pcon-pT181 atte(EcoRI & SpeI)||5.2||1.82||0.36
|-
|-
-
|Spinach-DT(X+P)|| ||8.0||1.87||0.25
+
|Spinach-DT(XbaI & PstI)||8.0||1.87||0.25
|-
|-
-
|RBS-lacZα-DT(X+P)|| ||10.3||1.71||0.54
+
|RBS-lacZ&alpha;-DT(XbaI & PstI)||10.3||1.71||0.54
|}
|}
{| class="wikitable"
{| class="wikitable"
Line 60: Line 58:
|1||1kbp ladder||
|1||1kbp ladder||
|-
|-
-
|2||Ptet-pT181-anti||E+P
+
|2||Ptet-pT181 antisense||EcoRI & PstI
|-
|-
-
|3||Ptet-pT181-anti||E+P
+
|3||Ptet-pT181 antisense||EcoRI & PstI
|-
|-
-
|5||pSB4K5||E+S
+
|5||pSB4K5||EcoRI & SpeI
|-
|-
-
|6||pSB4K5||E+S
+
|6||pSB4K5||EcoRI & SpeI
|}
|}
[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxafterxx.xxx]]<br>
[[File:igku_xxafterxx.xxx]]<br>
{| class="wikitable"
{| class="wikitable"
-
!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
+
!Name||concentration[&micro;g/mL]||260/280||260/230
|-
|-
-
|Ptet-pT181-anti(E+P)|| ||30.6||1.84||1.16
+
|Ptet-pT181 antisense(EcoRI & PstI)||30.6||1.84||1.16
|-
|-
-
|pSB4K5(E+S)|| ||21.8||1.87||0.98
+
|pSB4K5(EcoRI & SpeI)||21.8||1.87||0.98
|}
|}
{| class="wikitable"
{| class="wikitable"
Line 82: Line 80:
|1||1kbp ladder||
|1||1kbp ladder||
|-
|-
-
|2||Pcon-pT181 atte||S+P
+
|2||Pcon-pT181 attenuator||SpeI & PstI
|-
|-
-
|3||Pcon-pT181 atte||S+P
+
|3||Pcon-pT181 attenuator||SpeI & PstI
|-
|-
-
|5||Plac||S+P
+
|5||Plac||SpeI & PstI
|-
|-
-
|6||Plac||S+P
+
|6||Plac||SpeI & PstI
|-
|-
-
|8||pSB1C3||E+S
+
|8||pSB1C3||EcoRI & SpeI
|-
|-
-
|9||pSB1C3||E+S
+
|9||pSB1C3||EcoRI & SpeI
|-
|-
-
|11||pSB1C3||X+P
+
|11||pSB1C3||XbaI & PstI
|-
|-
-
|12||pSB1C3||X+P
+
|12||pSB1C3||XbaI & PstI
|}
|}
[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxafterxx.xxx]]<br>
[[File:igku_xxafterxx.xxx]]<br>
{| class="wikitable"
{| class="wikitable"
-
!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
+
!Name||concentration[&micro;g/mL]||260/280||260/230
|-
|-
-
|Pcon-pT181 atte(S+P)|| ||14.5||1.91||1.09
+
|Pcon-pT181 attenuator(SpeI & PstI)||14.5||1.91||1.09
|-
|-
-
|Plac(S+P)|| ||11.6||1.74||1.19
+
|Plac(SpeI & PstI)||11.6||1.74||1.19
|-
|-
-
|pSB1C3(E+S)|| ||5.7||1.74||0.34
+
|pSB1C3(EcoRI & SpeI)||5.7||1.74||0.34
|-
|-
-
|pSB1C3(X+P)|| ||5.6||2.00||0.37
+
|pSB1C3(XbaI & PstI)||5.6||2.00||0.37
|}
|}

Revision as of 22:27, 23 September 2013

Contents

Sep 8

Gel Extraction

No name

LaneDNAEnzyme
1100bp ladder
2RBS-lysis1-DTXbaI & PstI
3RBS-lysis1-DTXbaI & PstI
5RBS-lysis3-DTXbaI & PstI
6RBS-lysis3-DTXbaI & PstI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
RBS-lysis1-DT (XbaI & PstI)5.31.980.06
RBS-lysis3-DT (XbaI & PstI)5.31.980.06
LaneDNAEnzyme
1100bp ladder
3Pcon-pT181 atteEcoRI & SpeI
4Pcon-pT181 atteEcoRI & SpeI
6Spinach-DTXbaI & PstI
7Spinach-DTXbaI & PstI
9RBS-lacZα-DTXbaI & PstI
10RBS-lacZα-DTXbaI & PstI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
Pcon-pT181 atte(EcoRI & SpeI)5.21.820.36
Spinach-DT(XbaI & PstI)8.01.870.25
RBS-lacZα-DT(XbaI & PstI)10.31.710.54
LaneDNAEnzyme
11kbp ladder
2Ptet-pT181 antisenseEcoRI & PstI
3Ptet-pT181 antisenseEcoRI & PstI
5pSB4K5EcoRI & SpeI
6pSB4K5EcoRI & SpeI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
Ptet-pT181 antisense(EcoRI & PstI)30.61.841.16
pSB4K5(EcoRI & SpeI)21.81.870.98
LaneDNAEnzyme
11kbp ladder
2Pcon-pT181 attenuatorSpeI & PstI
3Pcon-pT181 attenuatorSpeI & PstI
5PlacSpeI & PstI
6PlacSpeI & PstI
8pSB1C3EcoRI & SpeI
9pSB1C3EcoRI & SpeI
11pSB1C3XbaI & PstI
12pSB1C3XbaI & PstI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
Pcon-pT181 attenuator(SpeI & PstI)14.51.911.09
Plac(SpeI & PstI)11.61.741.19
pSB1C3(EcoRI & SpeI)5.71.740.34
pSB1C3(XbaI & PstI)5.62.000.37

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
Pcon-pT181 anti243.01.901.81
Pcon-Spinach-DT253.71.571.51

Restriction Enzyme Digestion

じっけんしゃ

DNArestricion enzymerestriction enzymeBufferBSAMilliQtotal
2 cuts（°°） µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL

じっけんしゃ

DNArestricion enzymerestriction enzymeBufferBSAMilliQtotal
2 cuts（°°） µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL

Transformation

じっけんしゃ

NameSampleCompetent CellsTotalPlate
いでんしなんちゃらµLごにょごにょµLほにゃららµLこーせーぶっしつ
いかどうよう

Electrophoresis

じっけんしゃ

LaneSampleEnzyme1Enzyme2
（°Д°）
LaneSampleEnzyme1Enzyme2
（°Д°）
LaneSampleEnzyme1Enzyme2
（°Д°）

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experimentnamexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL
stateVectorInserterLigation High ver.2
experimentnamexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL

PCR

No name

genome DNAKOD plus10x bufferdNTPMgSO4SasA_fwd primerSasA_rev primerMilliQtotal
0.52.52.51.50.750.75 25
PreDenatureDenatureAnnealingExtensioncycle
　°C　°C　°C　°C--
（◎Д◎）　　　　

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
LaneDNAEnzyme
（＞ω・）b

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）
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