From 2013.igem.org
(Difference between revisions)
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| | ===Colony PCR=== | | ===Colony PCR=== |
| - | <div class="experiment">
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| - | <span class="author">No name</span>
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| - | {| class="wikitable"
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| - | !Sample||base pair
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| - | |-
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| - | |9/8 Plac-spinach-DT-(1)||659
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| - | |}
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| - | {| class="wikitable"
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| - | !PreDenature||Denature||Annealing||Extension||cycle
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| - | |-
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| - | |94°C||94°C||55°C||68°C||--
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| - | |-
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| - | |5m||30s||30s||40s||30cycles
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| - | |}
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| - | </div>
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| - | <div class="experiment">
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| - | {| class="wikitable"
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| - | !Sample||base pair
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| - | |-
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| - | |9/8 Plac-spinach-DT-(2)||659
| |
| - | |}
| |
| - | {| class="wikitable"
| |
| - | !PreDenature||Denature||Annealing||Extension||cycle
| |
| - | |-
| |
| - | |94°C||94°C||55°C||68°C||--
| |
| - | |-
| |
| - | |5m||30s||30s||40s||30cycles
| |
| - | |}
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| - | </div>
| |
| - | <div class="experiment">
| |
| - | {| class="wikitable"
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| - | !Sample||base pair
| |
| - | |-
| |
| - | |9/8 Pcon-pt181atte-DT-(1)||584
| |
| - | |}
| |
| - | {| class="wikitable"
| |
| - | !PreDenature||Denature||Annealing||Extension||cycle
| |
| - | |-
| |
| - | |94°C||94°C||55°C||68°C||--
| |
| - | |-
| |
| - | |5m||30s||30s||40s||30cycles
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| - | |}
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| - | </div>
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Revision as of 03:05, 24 September 2013
Sep 11
Tatsui
| pKaiBC(200p) | 10*buffer KOD plus | dNTPs | MgSO4 | primer mix(F&R) | KOD plus | MilliQ | total
|
| 0.5 | 2.5 | 2.5 | 1.5 | 1.5 | 0.5 | 16 | | 25
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 98°C | 60°C | 68°C | 30
|
| 2min | 10sec | 30sec | 30sec |
|
| pKaiBC(400p) | 10*buffer KOD plus | dNTPs | MgSO4 | primer mix(F&R) | KOD plus | MilliQ | total
|
| 1 | 2.5 | 2.5 | 1.5 | 1.5 | 0.5 | 15.5 | | 25
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 98°C | 60°C | 68°C | 30
|
| 2min | 10sec | 30sec | 30sec |
|
| pKaiBC(800p) | 10*buffer KOD plus | dNTPs | MgSO4 | primer mix(F&R) | KOD plus | MilliQ | total
|
| 2 | 2.5 | 2.5 | 1.5 | 1.5 | 0.5 | 14.5 | | 25
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 98°C | 60°C | 68°C | 30
|
| 2min | 10sec | 30sec | 30sec |
|
| pKaiBC(1ng) | 10*buffer KOD plus | dNTPs | MgSO4 | primer mix(F&R) | KOD plus | MilliQ | total
|
| 2.5 | 2.5 | 2.5 | 1.5 | 1.5 | 0.5 | 14 | | 25
|
| PreDenature | Denature | Annealing | Extension | cycle
|
| 94°C | 98°C | 60°C | 68°C | 30
|
| 2min | 10sec | 30sec | 30sec |
|
Colony PCR
"
