Team:BYU Provo/Notebook/Phage Purification/Winterexp/Period1/Exp/4.5PhageSpotTest

From 2013.igem.org

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: We plated together .5mL of BL21 with 1X LB TA.
: We plated together .5mL of BL21 with 1X LB TA.
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We separated the plates into different segments and then spot tested the phage.
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: We separated the plates into different segments and then spot tested the phage.
The plates were then put in a 37°C incubator.
The plates were then put in a 37°C incubator.

Revision as of 22:16, 17 June 2013


Phage Purification March - April Notebook: Experiments



Overview
March-April
May-June
July-August
September-October

4.5 Phage Spot Test


I) Purpose

Test for viable phage.

II) Expected Outcome

Plaques in the bacteria where phage can infect and kill.

III) Reagants Used

BL21
1x LB top agar
Phage: 14 T3
13 T5
10 T2
20OX171
40ØX174

IV) Actual Procedure

We plated together .5mL of BL21 with 1X LB TA.
We separated the plates into different segments and then spot tested the phage.

The plates were then put in a 37°C incubator.

V) Results

No plaques were seen on a uniform bacterial lawn. The control was clear of any contamination or plaques. The phage were not viable.


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