Template:Kyoto/Notebook/Sep 16

From 2013.igem.org

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(→Sep 16)
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{| class="wikitable"
{| class="wikitable"
!Sample||medium
!Sample||medium
 +
|}
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===Colony PCR===
 +
<!-- ここから -->
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<div class="experiment">
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<span class="author">No name</span>
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{| class="wikitable"
 +
!Sample||base pair
 +
|-
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|(｀・ω・´)||
 +
|}
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{| class="wikitable"
 +
!PreDenature||Denature||Annealing||Extension||cycle
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|-
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|　&deg;C||　&deg;C||　&deg;C||　&deg;C||--
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|-
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|（◎Д◎）　||　||　||　||
|}
|}
===Gel Extraction===
===Gel Extraction===

Revision as of 05:59, 24 September 2013

Contents

Sep 16

Liquid Culture

No name

Samplemedium

Colony PCR

No name

Samplebase pair
(｀・ω・´)
PreDenatureDenatureAnnealingExtensioncycle
　°C　°C　°C　°C--
（◎Д◎）　　　　

Gel Extraction

じっけんしゃ

LaneDNAEnzyme

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
(・▽・)　　

PCR

No name

genome DNAKOD plus10x bufferdNTPMgSO4SasA_fwd primerSasA_rev primerMilliQtotal
0.52.52.51.50.750.75 25
PreDenatureDenatureAnnealingExtensioncycle
　°C　°C　°C　°C--
（◎Д◎）　　　　

Electrophoresis

じっけんしゃ

LaneSampleEnzyme1Enzyme2
（°Д°）

File:Igku xxxxxx.xxx

Restriction Enzyme Digestion

じっけんしゃ

DNArestricion enzymerestriction enzymeBufferBSAMilliQtotal
2 cuts（°°） µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experimentnamexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL

PCR

No name

genome DNAKOD plus10x bufferdNTPMgSO4SasA_fwd primerSasA_rev primerMilliQtotal
0.52.52.51.50.750.75 25
PreDenatureDenatureAnnealingExtensioncycle
　°C　°C　°C　°C--
（◎Д◎）　　　　
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