Template:Kyoto/Notebook/Sep 16

From 2013.igem.org

(Difference between revisions)
(→Colony PCR)
(→Gel Extraction)
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{| class="wikitable"
{| class="wikitable"
!Lane||DNA||Enzyme
!Lane||DNA||Enzyme
 +
|-
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|1||1kb ladder
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|-
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|2||pT181 attenuator||X+P
 +
|-
 +
|3||pT181 attenuator||X+P
 +
|-
 +
|4||pT181 attenuator||X+P
 +
|-
 +
|6||pSB1C3||E+S
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|-
 +
|7||pSB1C3||E+S
 +
|-
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|8||pSB1C3||E+S
 +
|-
 +
|10||pSB4K5||X+P
 +
|-
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|11||pSB4K5||X+P
 +
|-
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|12||pSB4K5||X+P
 +
|-
|}
|}
[[File:igku_xxbeforexx.xxx]]<br>
[[File:igku_xxbeforexx.xxx]]<br>
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{| class="wikitable"
{| class="wikitable"
!Name||concentration[&micro;g/mL]||260/280||260/230
!Name||concentration[&micro;g/mL]||260/280||260/230
 +
|-
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|pT181 attenuator(X+P)|| ||8.2||1.62||0.11
 +
|-
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|pSB1C3(E+S)||49.5||1.48||0.18
 +
|-
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|pSB4K5(X+P)||18.1||1.73||0.57
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|-
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|DT(S+P)||70.4||1.36||1.02
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|-
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|aptamer12-1R-DT(S+P)||42.0||1.82||0.47
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|-
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|spinach-DT(S+P)||36.8||1.79||0.61
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|-
|}
|}
 +
===Miniprep===
===Miniprep===
<div class="experiment">
<div class="experiment">

Revision as of 06:10, 24 September 2013

Contents

Sep 16

Liquid Culture

No name

Samplemedium
pSB1C3+P-KaiBC①Plusgrow medium(+CP)
pSB1C3+P-KaiBC②Plusgrow medium(+CP)

Colony PCR

No name

Samplebase pair
pSB1C3+P-KaiBC①283
pSB1C3+P-KaiBC②283
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
2min30s30s18s30cycles

Gel Extraction

じっけんしゃ

LaneDNAEnzyme
11kb ladder
2pT181 attenuatorX+P
3pT181 attenuatorX+P
4pT181 attenuatorX+P
6pSB1C3E+S
7pSB1C3E+S
8pSB1C3E+S
10pSB4K5X+P
11pSB4K5X+P
12pSB4K5X+P

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Nameconcentration[µg/mL]260/280260/230
pT181 attenuator(X+P) 8.21.620.11
pSB1C3(E+S)49.51.480.18
pSB4K5(X+P)18.11.730.57
DT(S+P)70.41.361.02
aptamer12-1R-DT(S+P)42.01.820.47
spinach-DT(S+P)36.81.790.61

Miniprep

No name

DNAconcentration[µg/mL]260/280260/230
(・▽・)　　

PCR

No name

genome DNAKOD plus10x bufferdNTPMgSO4SasA_fwd primerSasA_rev primerMilliQtotal
0.52.52.51.50.750.75 25
PreDenatureDenatureAnnealingExtensioncycle
　°C　°C　°C　°C--
（◎Д◎）　　　　

Electrophoresis

じっけんしゃ

LaneSampleEnzyme1Enzyme2
（°Д°）

File:Igku xxxxxx.xxx

Restriction Enzyme Digestion

じっけんしゃ

DNArestricion enzymerestriction enzymeBufferBSAMilliQtotal
2 cuts（°°） µL µL µL µL µL µL µL
NC µL µL µL µL µL µL µL

Ligation

じっけんしゃ

stateVectorInserterLigation High ver.2
experimentnamexx µg/mLnamexx µg/mLxx µg/mL
NCnamexx µg/mLnamexx µg/mLxx µg/mL

PCR

No name

genome DNAKOD plus10x bufferdNTPMgSO4SasA_fwd primerSasA_rev primerMilliQtotal
0.52.52.51.50.750.75 25
PreDenatureDenatureAnnealingExtensioncycle
　°C　°C　°C　°C--
（◎Д◎）　　　　
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