Team:Peking

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Revision as of 07:08, 24 September 2013

Aromatics Scouts

A Comprehensive Biosensor Toolkit to Profile Aromatics in the Environment

Monitoring aromatics in the environment remains a substantial challenge today. Noting the power of biosensors for quick and convenient testing, Peking iGEM has developed a functionally comprehensive biosensor toolkit to profile aromatics in the environment.

Transcriptional regulators that each senses a specific class of aromatics were first bioinformatically determined; they were then utilized to build a comprehensive set of biosensor circuits. Characterization on the detection profiles of individual biosensors and their orthogonality/crosstalk prove that these biosensors are really high-performance to profile aromatics in water.

Moreover, for the ease of practical applications, two types of genetic devices were also developed as plug-ins for biosensors: "Adaptors", a set of conceptually novel devices to convert undetectable chemicals into detectable aromatics, and "Band-pass Filter", a "concentration filter" that allows the detection of analyte concentration within a specific range.

We expect that these novel biosensors, together with the plug-in devices, will serve as intriguing synthetic biological tools for diverse practical applications.

Mining aromatics-sensing Biobricks
from the genomic database

Sensor Mining

High-performance, profile-
specific biosensors

Biosensors

Convert the undetectable into
the detectable

Adaptors

Rapidly tell the concentration
of the analyte

Band-pass Filter

Resonate to bridge the gap
between minds

Human Practice