Template:Kyoto/Notebook/Sep 4

From 2013.igem.org

(Difference between revisions)
(→Colony PCR)
(→Gel Extraction)
Line 13: Line 13:
|1||100bp ladder||--
|1||100bp ladder||--
|-
|-
-
|2||Pcon||Spe1&Pst1
+
|2||Pcon||SpeI&PstI
|-
|-
-
|3||Pcon||Spe1&Pst1
+
|3||Pcon||SpeI&PstI
|-
|-
|4||--||--
|4||--||--
|-
|-
-
|5||Plac||Spe1&Pst1
+
|5||Plac||SpeI&PstI
|-
|-
-
|6||Plac||Spe1&Pst1
+
|6||Plac||SpeI&PstI
|-
|-
Line 48: Line 48:
|1||100bp ladder||--
|1||100bp ladder||--
|-
|-
-
|2||Ptet||Spe1&Pst1
+
|2||Ptet||SpeI&PstI
|-
|-
-
|3||Ptet||Spe1&Pst1
+
|3||Ptet||SpeI&PstI
|-
|-
|4||--||--
|4||--||--
|-
|-
-
|5||RBS-tetR-DT||Xba1&Pst1
+
|5||RBS-tetR-DT||XbaI&PstI
|-
|-
-
|6||RBS-tetR-DT||Xba1&Pst1
+
|6||RBS-tetR-DT||XbaI&PstI
|-
|-

Revision as of 08:57, 24 September 2013

Contents

Sep 4

Gel Extraction

No name

1

LaneDNAEnzyme
1100bp ladder--
2PconSpeI&PstI
3PconSpeI&PstI
4----
5PlacSpeI&PstI
6PlacSpeI&PstI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

</div>

No name

2

LaneDNAEnzyme
1100bp ladder--
2PtetSpeI&PstI
3PtetSpeI&PstI
4----
5RBS-tetR-DTXbaI&PstI
6RBS-tetR-DTXbaI&PstI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
（´ω｀）
（ΦωΦ^）

Master Plate

No name

NumberUse LB plate (+Amp)
48/9 BBa_J23100-3
58/9 BBa_J23100-4
68/9 BBa_J23100-5

Master Plate

Hirano

NumberUse LB plate
1JM109-1
2JM109-2
3JM109-3
4JM109-4


Liquid Culture

Hirano

Samplemedium
8/9 BBa_J23100-3Plusgrow medium(+Amp)

Liquid Culture

Hirano

Samplemedium
JM109-1(Master plate)LB medium

Colony PCR

Hirano

Samplebase pair
8/9 BBa_J23100 3--
8/9 BBa_J23100 4--
8/9 BBa_J23100 5--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s30s30cycles
Retrieved from "http://2013.igem.org/Template:Kyoto/Notebook/Sep_4"