Template:Kyoto/Notebook/Sep 4

From 2013.igem.org

(Difference between revisions)
(→Gel Extraction)
(→Colony PCR)
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|5min||30s||30s||30s||30cycles
|5min||30s||30s||30s||30cycles
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|}
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</div>
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===Gel Extraction===
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<div class="experiment">
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<span class="author">No name</span></div>
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{| class="wikitable"
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!Lane||DNA||Enzyme
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|-
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|1||100bp ladder||--
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|-
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|2||spinach-DT||XbaII&PstI
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|-
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|3||spinach-DT ||XbaII&PstI
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|-
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|4||--||--
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|-
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|5||RBS-lacZ&alpha;-DT||XbaI&PstI
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|-
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|6||RBS-lacZ&alpha;-DT||XbaI&PstI
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|}
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[[File:igku_xxbeforexx.xxx]]<br>
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[[File:igku_xxafterxx.xxx]]<br>
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{| class="wikitable"
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!Name||quantity||concentration[&micro;g/mL]||260/280||260/230
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|-
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|spinach-DT||--||2.1 ||2.10 ||-0.05
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|-
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|RBS-lacZ&alpha;-DT||-- ||3.3 ||2.59 ||-0.07
|}
|}
</div>
</div>

Revision as of 09:27, 24 September 2013

Contents

Sep 4

Gel Extraction

No name

1

LaneDNAEnzyme
1100bp ladder--
2PconSpeI&PstI
3PconSpeI&PstI
4----
5PlacSpeI&PstI
6PlacSpeI&PstI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
Pcon--13.2 1.99 -1.64
Plac-- 15.3 2.07 -o.39
No name

2

LaneDNAEnzyme
1100bp ladder--
2PtetSpeI&PstI
3PtetSpeI&PstI
4----
5RBS-tetR-DTXbaI&PstI
6RBS-tetR-DTXbaI&PstI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
Ptet-- 30.1 1.97 -0.97
RBS-tetR-DT-- 3.0 2.44 0.88

Gel Extraction

No name


LaneDNAEnzyme
1100bp ladder--
2spinach-DTXbaII&PstI
3spinach-DT NCXbaII&PstI
4RBS-lacZα-DTXbaI&PstI
5RBS-lacZα-DT NCXbaI&PstI
6100bp ladder --

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
spinach-DT--2.1 2.10 -0.05
RBS-lacZα-DT-- 3.3 2.59 -0.07

</div>

Master Plate

No name

NumberUse LB plate (+Amp)
48/9 BBa_J23100-3
58/9 BBa_J23100-4
68/9 BBa_J23100-5

Master Plate

Hirano

NumberUse LB plate
1JM109-1
2JM109-2
3JM109-3
4JM109-4


Liquid Culture

Hirano

Samplemedium
8/9 BBa_J23100-3Plusgrow medium(+Amp)

Liquid Culture

Hirano

Samplemedium
JM109-1(Master plate)LB medium

Colony PCR

Hirano

Samplebase pair
8/9 BBa_J23100 3--
8/9 BBa_J23100 4--
8/9 BBa_J23100 5--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s30s30cycles

Gel Extraction

No name


LaneDNAEnzyme
1100bp ladder--
2spinach-DTXbaII&PstI
3spinach-DT XbaII&PstI
4----
5RBS-lacZα-DTXbaI&PstI
6RBS-lacZα-DTXbaI&PstI

File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx

Namequantityconcentration[µg/mL]260/280260/230
spinach-DT--2.1 2.10 -0.05
RBS-lacZα-DT-- 3.3 2.59 -0.07

</div>

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