Template:Team:SydneyUni Australia/Calendar/Events List
From 2013.igem.org
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// -----------------Week 1 ---------------- | // -----------------Week 1 ---------------- | ||
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title: 'PCR analysis and GC analysis of DCA metabolism', | title: 'PCR analysis and GC analysis of DCA metabolism', | ||
start: new Date(2013, 5, 3), | start: new Date(2013, 5, 3), | ||
- | description: '<b>Members:</b> Colman, Hugh, Rob <br> <b>What we did:</b> We ran a gel of last Friday’s PCR. The PCR was of pBS(ToMO) extracted from Wood’s E. Coli TG1, with primers iGEM 1 & 2. The banding on the gel was obscure, potentially due to too much template and could not be used. <br><br> We used the Gas Chromatographer (GC) to look at our two DCA treatments of E. Coli (transformed with either pBBR or pBS(ToMO)). Both had pretty much the same reading but it is uncertain yet whether this is because pBS(ToMO) was lost or altered during the extended incubation from Week 2, or whether ToMO was unable to degrade DCA. We also set up standardised solutions of NaCl in KP buffer for a future Cl assay. | + | description: '<b>Members:</b> Colman, Hugh, Rob <br> <b>What we did:</b> We ran a gel of last Friday’s PCR. The PCR was of pBS(ToMO) extracted from Wood’s E. Coli TG1, with primers iGEM 1 & 2. The banding on the gel was obscure, potentially due to too much template and could not be used. <br><br> We used the Gas Chromatographer (GC) to look at our two DCA treatments of E. Coli (transformed with either pBBR or pBS(ToMO)). Both had pretty much the same reading but it is uncertain yet whether this is because pBS(ToMO) was lost or altered during the extended incubation from Week 2, or whether ToMO was unable to degrade DCA. We also set up standardised solutions of NaCl in KP buffer for a future Cl assay.' |
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// -----------------Week 4 ---------------- | // -----------------Week 4 ---------------- | ||
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url: 'http://google.com/' | url: 'http://google.com/' | ||
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}); | }); |
Revision as of 11:01, 24 September 2013