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Template:Kyoto/Notebook/Sep 15
From 2013.igem.org
(Difference between revisions)
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|experiment||9/13 Pcon(SpeI & PstI)||3.6 µL||9/12 RBS-lacZα-DT(XbaI & PstI)||4.7 µL||8 µL | |experiment||9/13 Pcon(SpeI & PstI)||3.6 µL||9/12 RBS-lacZα-DT(XbaI & PstI)||4.7 µL||8 µL | ||
|} | |} | ||
| + | </div> | ||
| + | <!-- ここまでをコピーしてね --> | ||
| + | ===Restriction Enzyme Digestion=== | ||
| + | <!-- こっから --> | ||
| + | <div class="experiment"> | ||
| + | <span class="author">じっけんしゃ</span> | ||
| + | {| class="wikitable" | ||
| + | ! ||DNA||EcorI||SpeI||Buffer||BSA||MilliQ||total | ||
| + | |- | ||
| + | |9/7Pcon-RBS-tetR-DT (EcoRI & SpeI)|| µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |- | ||
| + | |NC|| µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |} | ||
| + | <br> | ||
| + | {| class="wikitable" | ||
| + | ! ||DNA||EcoRI||SpeI||Buffer||BSA||MilliQ||total | ||
| + | |- | ||
| + | |9/12 Pcon-pT181 attenuator-DT (EcoRI & SpeI)|| µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |- | ||
| + | |NC|| µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |} | ||
| + | <br> | ||
| + | {| class="wikitable" | ||
| + | ! ||DNA||EcoRI||SpeI||Buffer||BSA||MilliQ||total | ||
| + | |- | ||
| + | |9/12 Pcon-apt12_1R-DT(EcoRI & SpeI)|| µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |- | ||
| + | |NC|| µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |} | ||
| + | <br> | ||
| + | {| class="wikitable" | ||
| + | ! ||DNA||EcoRI||SpeI||Buffer||BSA||MilliQ||total | ||
| + | |- | ||
| + | |9/8 Pcon-spin-DT (EcoRI & SpeI)|| µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |- | ||
| + | |NC|| µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |} | ||
| + | <br> | ||
| + | {| class="wikitable" | ||
| + | ! ||DNA||XbaI||PstI||Buffer||BSA||MilliQ||total | ||
| + | |- | ||
| + | |9/11 Pcon-pT181 anticodone-spin-DT(XbaI & PstI)|| µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |- | ||
| + | |NC|| µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |} | ||
| + | <br> | ||
| + | {| class="wikitable" | ||
| + | ! ||DNA||XbaI||PstI||Buffer||BSA||MilliQ||total | ||
| + | |- | ||
| + | |8/17 RBS-lacZα-DT (XbaI & PstI)|| µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |- | ||
| + | |NC|| µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |} | ||
| + | <br> | ||
| + | {| class="wikitable" | ||
| + | ! ||DNA||EcoRI||XbaI||Buffer||BSA||MilliQ||total | ||
| + | |- | ||
| + | |9/7 Ptet-RBS-LacZα-DT (EcoRI & XbaI)|| µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |- | ||
| + | |NC|| µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |} | ||
| + | <br> | ||
| + | {| class="wikitable" | ||
| + | |||
</div> | </div> | ||
<!-- ここまでをコピーしてね --> | <!-- ここまでをコピーしてね --> | ||
Revision as of 14:12, 24 September 2013
Contents |
Sep 15
Miniprep
| DNA | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| Pλ-luxI | 225 | 1.59 | 0.83 |
Liquid Culture
| Sample | medium |
|---|---|
| 9/13 Pλ-luxI-1 | |
| 9/7 pSB4K5-1 | |
| 9/11 Plac-3 |
Ligation
| state | Vector | Inserter | Ligation High ver.2 | ||
|---|---|---|---|---|---|
| experiment | 9/13 Pcon(SpeI & PstI) | 3.6 µL | 9/12 RBS-lacZα-DT(XbaI & PstI) | 4.7 µL | 8 µL |
Restriction Enzyme Digestion
| DNA | EcorI | SpeI | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 9/7Pcon-RBS-tetR-DT (EcoRI & SpeI) | µL | µL | µL | µL | µL | µL | µL |
| NC | µL | µL | µL | µL | µL | µL | µL |
| DNA | EcoRI | SpeI | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 9/12 Pcon-pT181 attenuator-DT (EcoRI & SpeI) | µL | µL | µL | µL | µL | µL | µL |
| NC | µL | µL | µL | µL | µL | µL | µL |
| DNA | EcoRI | SpeI | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 9/12 Pcon-apt12_1R-DT(EcoRI & SpeI) | µL | µL | µL | µL | µL | µL | µL |
| NC | µL | µL | µL | µL | µL | µL | µL |
| DNA | EcoRI | SpeI | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 9/8 Pcon-spin-DT (EcoRI & SpeI) | µL | µL | µL | µL | µL | µL | µL |
| NC | µL | µL | µL | µL | µL | µL | µL |
| DNA | XbaI | PstI | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 9/11 Pcon-pT181 anticodone-spin-DT(XbaI & PstI) | µL | µL | µL | µL | µL | µL | µL |
| NC | µL | µL | µL | µL | µL | µL | µL |
| DNA | XbaI | PstI | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 8/17 RBS-lacZα-DT (XbaI & PstI) | µL | µL | µL | µL | µL | µL | µL |
| NC | µL | µL | µL | µL | µL | µL | µL |
| DNA | EcoRI | XbaI | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 9/7 Ptet-RBS-LacZα-DT (EcoRI & XbaI) | µL | µL | µL | µL | µL | µL | µL |
| NC | µL | µL | µL | µL | µL | µL | µL |
