Template:Kyoto/Notebook/Sep 15

(Difference between revisions)

Revision as of 15:02, 24 September 2013

No name

DNA	concentration[µg/mL]	260/280	260/230
Pλ-luxI	225	1.59	0.83

No name

じっけんしゃ

state	Vector		Inserter		Ligation High ver.2
experiment	9/13 Pcon(SpeI & PstI)	3.6 µL	9/12 RBS-lacZα-DT(XbaI & PstI)	4.7 µL	8 µL

No Name

	DNA	EcorI	SpeI	Buffer	BSA	MilliQ	total
9/7Pcon-RBS-tetR-DT (EcoRI & SpeI)	15 µL	1 µL	1 µL	3 µL	3 µL	6 µL	30 µL
NC	0.7 µL	0 µL	0 µL	1 µL	1 µL	7.3 µL	10 µL

	DNA	EcoRI	SpeI	Buffer	BSA	MilliQ	total
9/12 Pcon-pT181 attenuator-DT (EcoRI & SpeI)	6.8 µL	1 µL	1 µL	3 µL	3 µL	15.2 µL	30 µL
NC	0.3 µL	0 µL	0 µL	1 µL	1 µL	7.7 µL	10 µL

	DNA	EcoRI	SpeI	Buffer	BSA	MilliQ	total
9/12 Pcon-apt12_1R-DT(EcoRI & SpeI)	6.6 µL	1 µL	1 µL	3 µL	3 µL	15.4 µL	30 µL
NC	0.3 µL	0 µL	0 µL	1 µL	1 µL	7.7 µL	10 µL

	DNA	EcoRI	SpeI	Buffer	BSA	MilliQ	total
9/8 Pcon-spin-DT (EcoRI & SpeI)	7.6 µL	1 µL	1 µL	3 µL	3 µL	15.4 µL	30 µL
NC	0.3 µL	0 µL	0 µL	1 µL	1 µL	7.7 µL	10 µL

	DNA	XbaI	PstI	Buffer	BSA	MilliQ	total
9/11 Pcon-pT181 anticodone-spin-DT(XbaI & PstI)	7.2 µL	1 µL	1 µL	3 µL	3 µL	14.8 µL	30 µL
NC	0.4 µL	0 µL	0 µL	1 µL	1 µL	7.6 µL	10 µL

	DNA	XbaI	PstI	Buffer	BSA	MilliQ	total
8/17 RBS-lacZα-DT (XbaI & PstI)	5.3 µL	1 µL	1 µL	3 µL	3 µL	16.7 µL	30 µL
NC	0.5 µL	0 µL	0 µL	1 µL	1 µL	7.5 µL	10 µL

	DNA	EcoRI	XbaI	Buffer	BSA	MilliQ	total
9/7 Ptet-RBS-LacZα-DT (EcoRI & XbaI)	2.9 µL	1 µL	1 µL	3 µL	3 µL	18.5 µL	30 µL
NC	1.5 µL	0 µL	0 µL	1 µL	1 µL	7 µL	10 µL

No Name

Lane	Sample	Enzyme1	Enzyme2
1,14
2	Pcon-RBS-tetR-DT	EcoRI	SpeI
3	Pcon-RBS-tetR-DT
4	Pcon-pT181 attenuator-DT	EcoRI	SpeI
5	Pcon-pT181 attenuator-DT
6	Pcon-apt12-1R-DT	EcoRI	SpeI
7	Pcon-apt12-1R-DT
8	Pcon-spin-DT	EcoRI	SpeI
9	Pcon-spin-DT
10	Pcon-pT181 antisense-spin-DT	XbaI	PstI
11	Pcon-pT181 antisense-spin-DT
12	RBS-lacZα-DT	XbaI	PstI
13	RBS-lacZα-DT
15	Ptet-RBS-lacZα-DT	EcoRI	XbaI
16	Ptet-RBS-lacZα-DT
17	1kbp ladder

No Name 1

2

Name	concentration[µg/mL]	260/280	260/230
9/15 Ptet-RBS-lacZα-DT (EcoRI & SpeI)	1.9	1.08	0.77
9/15 Pcon-RBS-tetR-DT (EcoRI & SpeI)	5.9	1.30	0.17
9/15 RBS-lacZα-DT (XbaI & PstI)	1.0	0.85	0.44
Pcon-pT181 anticodone-spin-DT (XbaI & PstI)	1.3	0.95	0.28
Pcon-spin-DT	0.9	1.37	0.05
Pcon-apt12_1R-DT	1.7	1.42	0.29
Pcon-pT181 attenuator-DT (EcoRI & SpeI)	2.1	1.53	0.46

@@ Line 144: / Line 144: @@
 </div>
 <!-- ここまでをコピーしてね -->
+===Gel Extraction===
+<div class="experiment">
+<span class="author">No Name</span>
+{| class="wikitable"
+!Lane||DNA||Enzyme
+|-
+|1 ||100bp ladder ||
+|-
+|2~4 ||Pcon-pT181 attenuator-DT ||EcoRI & SpeI
+|-
+|6~8 ||Pcon-apt12_1R-DT ||
+|-
+|10~12 ||Pcon-spin-DT ||
+|}
+{| class="wikitable"
+!Lane||DNA||Enzyme
+|-
+|1 ||100bp ladder ||
+|-
+|2~4 ||Pcon-pT181 anticodone-spin-DT ||XbaI & PstI
+|-
+|6~8 ||RBS-lacZ&alpha;-DT ||XbaI & PstI
+|-
+|10~12 ||Pcon-RBS-tetR-DT ||EcoRI & SpeI
+|}
+[[File:igku_xxbeforexx.xxx]]<br>
+[[File:igku_xxafterxx.xxx]]<br>
+{| class="wikitable"
+!Name||concentration[&micro;g/mL]||260/280||260/230
+|-
+|9/15 Ptet-RBS-lacZ&alpha;-DT (EcoRI & SpeI) ||1.9 ||1.08 ||0.77
+|-
+|9/15 Pcon-RBS-tetR-DT (EcoRI & SpeI) ||5.9 ||1.30 ||0.17
+|-
+|9/15 RBS-lacZ&alpha;-DT (XbaI & PstI) ||1.0 ||0.85 ||0.44
+|-
+|Pcon-pT181 anticodone-spin-DT (XbaI & PstI) ||1.3 ||0.95 ||0.28
+|-
+|Pcon-spin-DT ||0.9 ||1.37 ||0.05
+|-
+|Pcon-apt12_1R-DT ||1.7 ||1.42 ||0.29
+|-
+|Pcon-pT181 attenuator-DT (EcoRI & SpeI) ||2.1 ||1.53 ||0.46
+|}
+</div>