Template:Kyoto/Notebook/Aug 15

(Difference between revisions)

Revision as of 01:09, 25 September 2013

Nakamoto

Nakamoto

Name	Sample	Competent Cells	Total	Plate
BBa_I0500(Pbad/araC)	2	17	19	LB (+Kan)
BBa_F1610(RBS-luxI-DT)	2	17	19	LB (+Amp)
BBa_R0061(Plux)	2	17	19	LB (+CP)
BBa_I13504(RBS-GFP-DT)	2	17	19	LB (+CP)
BBa_I0462(RBS-luxR-DT)	2	17	19	LB (+Amp)
BBa_K117000(lysis1)	2	17	19	LB (+CP)
BBa_K112806(lysis2)	2	17	19	LB (+CP)
BBa_K748002(lysis3)	2	17	19	LB (+CP)
BBa_B0015(DT)	2	17	19	LB (+CP)
BBa_Q04400(RBS-tetR-DT-Ptet)	2	17	19	LB (+Kan)
BBa_I732020(RBS-lacZα-DT)	2	17	19	LB (+CP)
BBa_J23100	2	17	19	LB (+Amp)

No name

DNA	concentration[µg/mL]	260/280	260/230
BBa_B0034(1)	84.1	2.11	2.01
BBa_B0034(2)	86.9	2.03	2.02
BBa_R0011(1)	78.3	2.26	2.02
BBa_R0011(2)	88.9	2.24	2.02
BBa_P0412(1)	15.61	2.10	1.93
BBa_P0412(2)	12.93	2.18	1.91

@@ Line 20: / Line 20: @@
 </div>
-===LB Medium Plate===
+===Transformation===
 <div class="experiment">
-<span class="author">Nakamoto & Tatsui</span>
+<span class="author">Nakamoto</span>
 {| class="wikitable"
-!volume||200ml
+!Name||Sample||Competent Cells||Total||Plate
 |-
-|Bacto T2ypton || 2g
+|BBa_I0500(Pbad/araC)||2||17||19||LB (+Kan)
 |-
-|Bacto yeast extract ||1g
+|BBa_F1610(RBS-luxI-DT)||2||17||19||LB (+Amp)
 |-
-|NaCl||1g
+|BBa_R0061(Plux)||2||17||19||LB (+CP)
 |-
-|0.4&micro; NaOHaq||50&micro;L
+|BBa_I13504(RBS-GFP-DT)||2||17||19||LB (+CP)
 |-
-|Agar Pouder||2g
+|BBa_I0462(RBS-luxR-DT)||2||17||19||LB (+Amp)
+|-
+|BBa_K117000(lysis1)||2||17||19||LB (+CP)
+|-
+|BBa_K112806(lysis2)||2||17||19||LB (+CP)
+|-
+|BBa_K748002(lysis3)||2||17||19||LB (+CP)
+|-
+|BBa_B0015(DT)||2||17||19||LB (+CP)
+|-
+|BBa_Q04400(RBS-tetR-DT-Ptet)||2||17||19||LB (+Kan)
+|-
+|BBa_I732020(RBS-lacZ&alpha;-DT)||2||17||19||LB (+CP)
+|-
+|BBa_J23100||2||17||19||LB (+Amp)
 |}
 </div>
 ===Miniprep===
 <div class="experiment">