Template:Kyoto/Notebook/Aug 16

From 2013.igem.org

(Difference between revisions)
(→Electrophoresis)
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===Transformation===
===Transformation===
-
<!-- こっから -->
 
<div class="experiment">
<div class="experiment">
-
<span class="author">じっけんしゃ</span>
+
<span class="author">Hirano</span>
{| class="wikitable"
{| class="wikitable"
!Name||Sample||Competent Cells||Total||Plate
!Name||Sample||Competent Cells||Total||Plate
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|pBR322||1&micro;L||50&micro;L(takara)||51&micro;L||Amp
|pBR322||1&micro;L||50&micro;L(takara)||51&micro;L||Amp
|-
|-
-
|Pbad araC||2&micro;L|||20&micro;L||22&micro;L||K
+
|Pbad/araC||2&micro;L|||20&micro;L||22&micro;L||K
|-
|-
|RBS-luxI-DT||2&micro;L|||20&micro;L||22&micro;L||K
|RBS-luxI-DT||2&micro;L|||20&micro;L||22&micro;L||K
|}
|}
 +
</div>
===Liquid Culture===
===Liquid Culture===

Revision as of 03:37, 25 September 2013

Contents

Aug 16

Colony PCR

Tatsui and Ashida

Samplebase pair
8/15 BBa_I13504(RBS-GFP-DT)(1)1174
8/15 BBa_I13504(RBS-GFP-DT)(2)1174
8/15 BBa_I0462(RBS-luxR-DT)(1)1174
8/15 BBa_I0462(RBS-luxR-DT)(2)1174
8/15 BBa_K748002(lysis3)1053
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s1min30cycles
Samplebase pair
8/15 BBa_R0061(Plux)344
8/15 BBa_K117000(lysis1)(1)456
8/15 BBa_K117000(lysis1)(2)456
8/15 BBa_B0015(DT)443
8/15 BBa_I732020(RBS-lacZα-DT)703
negative control--
PreDenatureDenatureAnnealingExtensioncycle
94°C94°C55°C68°C--
5min30s30s40s30cycles

Electrophoresis

No name

LaneSample
1100bp ladder
2BBa_I13504(RBS-GFP-DT) -(1)
3BBa_I13504(RBS-GFP-DT) -(2)
4BBa_I0462(RBS-luxR-DT) -(1)
5BBa_I0462(RBS-luxR-DT) -(2)
6BBa_K748002(lysis3)
7NC
8100bp ladder

File:Igku xxxxxx.xxx

LaneSample
1100bp ladder
2BBa_R0061(Plux)
3BBa_K117000(lysis1) -(1)
4BBa_K117000(lysis1) -(1)
5BBa_B0015(DT)
6BBa_I732020(RBS-lacZα-DT)
7NC
8100bp ladder

File:Igku xxxxxx.xxx

Master Plate

Tatsui

NumberUse LB plate (+CP)
1BBa_I13504(RBS-GFP-DT)(1)
2BBa_I13504(RBS-GFP-DT)(2)
3BBa_K748002(lysis3)
4BBa_R0061(Plux)
5BBa_K117000(lysis1)(1)
6BBa_K117000(lysis1)(2)
7BBa_B0015(DT)
8BBa_I732020(RBS-lacZα-DT)
NumberUse LB plate (+Amp)
1BBa_I0462(RBS-luxR-DT)(1)
2BBa_I0462(RBS-luxR-DT)(2)

Transformation

Hirano

NameSampleCompetent CellsTotalPlate
pBR3221µL100µL(XL-10gold)101µLAmp
pBR3221µL50µL(takara)51µLAmp
Pbad/araC2µL20µL22µLK
RBS-luxI-DT2µL20µL22µLK

Liquid Culture

A

Samplemedium
8/15 BBa_I13504(RBS-GFP-DT))(1)Plusgrow medium (+CP)
8/15 BBa_I13504(RBS-GFP-DT)(2)Plusgrow medium (+CP)
8/15 BBa_I0462(RBS-luxR-DT)(2)Plusgrow medium (+Amp)
8/15 BBa_K748002(lysis3)Plusgrow medium (+CP)
8/15 BBa_R0061(Plux)Plusgrow medium (+CP)
8/15 BBa_K117000(lysis1)(1)Plusgrow medium (+CP)
8/15 BBa_K117000(lysis1)(2)Plusgrow medium (+CP)
8/15 BBa_B0015(DT)Plusgrow medium (+CP)
8/15 BBa_I732020(RBS-lacZα-DT)Plusgrow medium (+CP)
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