Team:Washington/iGEMVectors

From 2013.igem.org

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<li><a href = "https://static.igem.org/mediawiki/2013/5/59/IGEM_pSB3T5%28std%29.gb" pSB1C3(std).gb></li>
<li><a href = "https://static.igem.org/mediawiki/2013/5/59/IGEM_pSB3T5%28std%29.gb" pSB1C3(std).gb></li>
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<li><a href = "https://2013.igem.org/Team:Washington/HEAT_SHOCK_CHEM_TRANS">6) Heat shock/chemical competent transformation</a></li>
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Revision as of 04:14, 25 September 2013



iGEM Vector Information

  • 6) Heat shock/chemical competent transformation
    • Vector Copy Number Equivalent Antibiotic Length Map
    pSB1C3 100-300 ColE1,pMB1(pUC19) Chloramphenicol 2070 bp Map:
    pSB3K3 20-30 p15A(pMR101) Kanamycin 2750 bp Map
    pSB4A5 ~5 RepA(pSC101) Ampicillin 2037 bp Map
    pSB3T5 20-30 p15A(pMR101) Tetracycline 3252 bp Map


    Vector Equivalency Chart
    Source Vector Origin Copy Number Antibiotic Biobrick Equivalent Antibiotic
    pJT119b ColE1,pMB1 100-300 Chloramphenicol pSB1C3 Chloramphenicol
    pPLPCB p15A 20-30 Spectinomycin pSB3T5 Tetracycline
    pEO100c pSC101 ~5 Ampicillin pSB4A5 Ampicillin
    pJT106b pSC101 ~5 Ampicillin pSB4A5 Ampicillin

    Retrieved from "http://2013.igem.org/Team:Washington/iGEMVectors"