Template:Kyoto/Notebook/Sep 13

From 2013.igem.org

(Difference between revisions)
(→Sep 13)
(→Sep 13)
Line 13: Line 13:
|-
|-
|9/12 Fusion3in2 attenuator-1||231.9||1.81||1.31
|9/12 Fusion3in2 attenuator-1||231.9||1.81||1.31
 +
|}
 +
</div>
 +
 +
===Gel Extraction===
 +
<div class="experiment">
 +
<span class="author">Tatsui,Ashida</span>
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||1kb ladder||--
 +
|-
 +
|2||RBS-lysis1-DT||XbaI&PstI
 +
|-
 +
|3||RBS-lysis1-DT||XbaI&PstI
 +
|-
 +
|4||RBS-lysis1-DT||XbaI&PstI
 +
|}
 +
 +
[[File:igku_Aug19electrophoresis3]]<br>
 +
[[File:igku_Aug19electrophoresis4]]<br>
 +
{| class="wikitable"
 +
!Name||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|RBS-lacZ&alpha;-DT(EcoRI&SpeI)||9.8||1.50||0.39
 +
|-
 +
|Pcon-RBS-lacZ&alpha;-DT(EcoRI&SpeI)||11.2||1.47||0.08
 +
|-
 +
|}
 +
</div>
 +
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||1kb ladder||--
 +
|-
 +
|3||RBS-lysis2-DT||XbaI&PstI
 +
|-
 +
|4||RBS-lysis2-DT||XbaI&PstI
 +
|-
 +
|5||RBS-lysis2-DT||XbaI&PstI
 +
|}
 +
 +
[[File:igku_Aug19electrophoresis3]]<br>
 +
[[File:igku_Aug19electrophoresis4]]<br>
 +
{| class="wikitable"
 +
!Name||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|RBS-lacZ&alpha;-DT(EcoRI&SpeI)||9.8||1.50||0.39
 +
|-
 +
|Pcon-RBS-lacZ&alpha;-DT(EcoRI&SpeI)||11.2||1.47||0.08
 +
|-
 +
|}
 +
</div>
 +
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|3||RBS-lysis3-DT||XbaI&PstI
 +
|-
 +
|4||RBS-lysis3-DT||XbaI&PstI
 +
|-
 +
|5||RBS-lysis3-DT||XbaI&PstI
 +
|-
 +
|6||1kb ladder||--
 +
|}
 +
 +
[[File:igku_Aug19electrophoresis3]]<br>
 +
[[File:igku_Aug19electrophoresis4]]<br>
 +
{| class="wikitable"
 +
!Name||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|RBS-lacZ&alpha;-DT(EcoRI&SpeI)||9.8||1.50||0.39
 +
|-
 +
|Pcon-RBS-lacZ&alpha;-DT(EcoRI&SpeI)||11.2||1.47||0.08
 +
|-
 +
|}
 +
</div>
 +
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|2||1kb ladder||--
 +
|-
 +
|3||pSB1C3||EcoRI&SpeI
 +
|-
 +
|4||pSB1C3||EcoRI&SpeI
 +
|-
 +
|5||pSB1C3||EcoRI&SpeI
 +
 +
|}
 +
 +
[[File:igku_Aug19electrophoresis3]]<br>
 +
[[File:igku_Aug19electrophoresis4]]<br>
 +
{| class="wikitable"
 +
!Name||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|RBS-lacZ&alpha;-DT(EcoRI&SpeI)||9.8||1.50||0.39
 +
|-
 +
|Pcon-RBS-lacZ&alpha;-DT(EcoRI&SpeI)||11.2||1.47||0.08
 +
|-
 +
|}
 +
</div>
 +
 +
{| class="wikitable"
 +
!Lane||DNA||Enzyme
 +
|-
 +
|1||1kb ladder||--
 +
|-
 +
|2||pSB1C3||XbaI&PstI
 +
|-
 +
|3||pSB1C3||XbaI&PstI
 +
|-
 +
|4||pSB1C3||XbaI&PstI
 +
|}
 +
 +
[[File:igku_Aug19electrophoresis3]]<br>
 +
[[File:igku_Aug19electrophoresis4]]<br>
 +
{| class="wikitable"
 +
!Name||concentration[&micro;g/mL]||260/280||260/230
 +
|-
 +
|RBS-lacZ&alpha;-DT(EcoRI&SpeI)||9.8||1.50||0.39
 +
|-
 +
|Pcon-RBS-lacZ&alpha;-DT(EcoRI&SpeI)||11.2||1.47||0.08
 +
|-
|}
|}
</div>
</div>

Revision as of 06:37, 25 September 2013

Sep 13

Miniprep

Nakamoto

DNAconcentration[µg/mL]260/280260/230
9/12 Fusion6 antisense245.91.881.41
9/12 Plux-3115.51.581.32
9/12 Plux-4125.31.931.44
9/12 Fusion3in2 attenuator-1231.91.811.31

Gel Extraction

Tatsui,Ashida

LaneDNAEnzyme
11kb ladder--
2RBS-lysis1-DTXbaI&PstI
3RBS-lysis1-DTXbaI&PstI
4RBS-lysis1-DTXbaI&PstI

File:Igku Aug19electrophoresis3
File:Igku Aug19electrophoresis4

Nameconcentration[µg/mL]260/280260/230
RBS-lacZα-DT(EcoRI&SpeI)9.81.500.39
Pcon-RBS-lacZα-DT(EcoRI&SpeI)11.21.470.08
LaneDNAEnzyme
11kb ladder--
3RBS-lysis2-DTXbaI&PstI
4RBS-lysis2-DTXbaI&PstI
5RBS-lysis2-DTXbaI&PstI

File:Igku Aug19electrophoresis3
File:Igku Aug19electrophoresis4

Nameconcentration[µg/mL]260/280260/230
RBS-lacZα-DT(EcoRI&SpeI)9.81.500.39
Pcon-RBS-lacZα-DT(EcoRI&SpeI)11.21.470.08

</div>

LaneDNAEnzyme
3RBS-lysis3-DTXbaI&PstI
4RBS-lysis3-DTXbaI&PstI
5RBS-lysis3-DTXbaI&PstI
61kb ladder--

File:Igku Aug19electrophoresis3
File:Igku Aug19electrophoresis4

Nameconcentration[µg/mL]260/280260/230
RBS-lacZα-DT(EcoRI&SpeI)9.81.500.39
Pcon-RBS-lacZα-DT(EcoRI&SpeI)11.21.470.08

</div>

LaneDNAEnzyme
21kb ladder--
3pSB1C3EcoRI&SpeI
4pSB1C3EcoRI&SpeI
5pSB1C3EcoRI&SpeI

File:Igku Aug19electrophoresis3
File:Igku Aug19electrophoresis4

Nameconcentration[µg/mL]260/280260/230
RBS-lacZα-DT(EcoRI&SpeI)9.81.500.39
Pcon-RBS-lacZα-DT(EcoRI&SpeI)11.21.470.08

</div>

LaneDNAEnzyme
11kb ladder--
2pSB1C3XbaI&PstI
3pSB1C3XbaI&PstI
4pSB1C3XbaI&PstI

File:Igku Aug19electrophoresis3
File:Igku Aug19electrophoresis4

Nameconcentration[µg/mL]260/280260/230
RBS-lacZα-DT(EcoRI&SpeI)9.81.500.39
Pcon-RBS-lacZα-DT(EcoRI&SpeI)11.21.470.08

</div>

Retrieved from "http://2013.igem.org/Template:Kyoto/Notebook/Sep_13"