Template:Kyoto/Notebook/Sep 3

From 2013.igem.org

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===Miniprep===
===Miniprep===
<div class="experiment">
<div class="experiment">
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<span class="author">No name</span>
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<span class="author">Tatsui</span>
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{|class="wikitable"  
!DNA||concentration[&micro;g/mL]||260/280||260/230  
!DNA||concentration[&micro;g/mL]||260/280||260/230  

Revision as of 09:27, 25 September 2013

Contents

Sep 3

Electrophoresis

Kozima

LaneSample
1100bp ladder
2pT181 attenuater(2)-DT2(738)
3Ptet-RBS-lacZα-DT-2(756)
4Ptet-RBS-lacZα-DT-3
5Ptet-RBS-lacZα-DT-4
6Ptet-RBS-lacZα-DT-5
7Ptet-RBS-lacZα-DT-6
8RBS-lysis2-DT-2(985)
9RBS-lysis2-DT-3
10100bp ladder

File:Igku xxxxxx.xxx

Miniprep

Tatsui

DNAconcentration[µg/mL]260/280260/230
9/2 14:00 pT181 attenuator418.41.821.80
9/2 14:00 pT181 antisense362.21.921.96
9/2 14:00 Spinach438.31.861.99
9/2 14:00 tetR-apt 12_1R567.51.901.99
9/2 21:00 pT181 attenuater320.91.902.15
9/2 21:00 Spinach457.01.882.24

Liquid Culture

Restriction Enzyme Digestion

PCR

Mutatation PCR

Colony PCR

Restriction Enzyme Digestion

Gel Extraction

Liquid Culture

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