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Template:Kyoto/Notebook/Sep 3
From 2013.igem.org
(Difference between revisions)
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===Restriction Enzyme Digestion=== | ===Restriction Enzyme Digestion=== | ||
| + | <div class="experiment"> | ||
| + | <span class="author">Kozima</span> | ||
| + | {| class="wikitable" | ||
| + | ! ||DNA||restricion enzyme||restriction enzyme||Buffer||BSA||MilliQ||total | ||
| + | |- | ||
| + | |2 cuts||（°°） µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |- | ||
| + | |NC|| µL|| µL|| µL|| µL|| µL|| µL|| µL | ||
| + | |} | ||
| + | </div> | ||
| + | |||
===PCR=== | ===PCR=== | ||
===Mutatation PCR=== | ===Mutatation PCR=== | ||
Revision as of 09:50, 25 September 2013
Contents |
Sep 3
Electrophoresis
| Lane | Sample |
|---|---|
| 1 | 100bp ladder |
| 2 | pT181 attenuater(2)-DT2(738) |
| 3 | Ptet-RBS-lacZα-DT-2(756) |
| 4 | Ptet-RBS-lacZα-DT-3 |
| 5 | Ptet-RBS-lacZα-DT-4 |
| 6 | Ptet-RBS-lacZα-DT-5 |
| 7 | Ptet-RBS-lacZα-DT-6 |
| 8 | RBS-lysis2-DT-2(985) |
| 9 | RBS-lysis2-DT-3 |
| 10 | 100bp ladder |
Miniprep
| DNA | concentration[µg/mL] | 260/280 | 260/230 |
|---|---|---|---|
| 9/2 14:00 pT181 attenuator | 418.4 | 1.82 | 1.80 |
| 9/2 14:00 pT181 antisense | 362.2 | 1.92 | 1.96 |
| 9/2 14:00 Spinach | 438.3 | 1.86 | 1.99 |
| 9/2 14:00 tetR-apt 12_1R | 567.5 | 1.90 | 1.99 |
| 9/2 21:00 pT181 attenuater | 320.9 | 1.90 | 2.15 |
| 9/2 21:00 Spinach | 457.0 | 1.88 | 2.24 |
Liquid Culture
| Sample | medium |
|---|---|
| Spinach-DT(9/2 master plate) | plusgrow(CP)4ml |
Restriction Enzyme Digestion
| DNA | restricion enzyme | restriction enzyme | Buffer | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 2 cuts | （°°） µL | µL | µL | µL | µL | µL | µL |
| NC | µL | µL | µL | µL | µL | µL | µL |
