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From 2013.igem.org

Revision as of 10:57, 24 September 2013 (view source) Ejpostema (Talk | contribs) ← Older edit		Revision as of 11:15, 25 September 2013 (view source) Ejpostema (Talk | contribs) Newer edit →
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-	Our silk we have developed was based on the article of Brooks et al. (1), but the codon optimization was done by the team itself using software written by one of the team members.	+	Our silk we have developed was based on the article of Brooks et al. [1], but the codon optimization was done by the team itself using software written by one of the team members.
	We improved the backbone made by the iGEM team from Munich in 2012 and used this backbone for our constructs.		We improved the backbone made by the iGEM team from Munich in 2012 and used this backbone for our constructs.
	We also validated that biobrick (Bba_E0840) works in <i>bacillus subtilis </i>(Figure 1).		We also validated that biobrick (Bba_E0840) works in <i>bacillus subtilis </i>(Figure 1).
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-	The coating mechanism was developed by the team using article (2). The knock-out chains where shipped from the university of Illinois and Buenos Aires .	+	The coating mechanism was developed by the team using article [2]. The knock-out chains where shipped from the university of Illinois and Buenos Aires .
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	<h2> References </h2>		<h2> References </h2>
-	1: Brooks et al (complete ref)	+	1: Brooks, Amanda E., Shane M. Stricker, Sangeeta B. Joshi, Timothy J. Kamerzell, C. Russell Middaugh, and Randolph V. Lewis. "Properties of Synthetic Spider Silk Fibers Based on Argiope Aurantia MaSp2." Biomacromolecules 9.6 (2008): 1506-510.
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Revision as of 11:15, 25 September 2013

Attributions

We have thought of this project by ourselves, so it was not an assignment from one of our supervisors. The supervisors and advisers only had influence on the team as a consultant. All the design, labwork and modeling was done by the team itself and started in June. We also took care of the sponsoring and all other organisational issues, made our own presentations and poster.

Our silk we have developed was based on the article of Brooks et al. [1], but the codon optimization was done by the team itself using software written by one of the team members. We improved the backbone made by the iGEM team from Munich in 2012 and used this backbone for our constructs. We also validated that biobrick (Bba_E0840) works in bacillus subtilis (Figure 1).

The coating mechanism was developed by the team using article [2]. The knock-out chains where shipped from the university of Illinois and Buenos Aires .

References

1: Brooks, Amanda E., Shane M. Stricker, Sangeeta B. Joshi, Timothy J. Kamerzell, C. Russell Middaugh, and Randolph V. Lewis. "Properties of Synthetic Spider Silk Fibers Based on Argiope Aurantia MaSp2." Biomacromolecules 9.6 (2008): 1506-510. 2: