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Team:UCL/Project/Protocols
From 2013.igem.org
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This protocol is for the stable transfection of eukaryotic adherent cell types in a 6-well plate, with plasmid DNA that ought to be of as high a purity as possible. | This protocol is for the stable transfection of eukaryotic adherent cell types in a 6-well plate, with plasmid DNA that ought to be of as high a purity as possible. | ||
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| + | <b>1)</b> The day before transfection, seed 0.9-4x10^5 cell per well of the six well plate with 2ml of appropriate growth medium. This should produce a confluence of 40-80% for the next day's transfection. | ||
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| + | <b>2)</b> Incubate cells in their normal growth conditions (37^0 C and 5% CO2). | ||
| + | </p> | ||
| + | <p class="body_text"> | ||
| + | <b>3)</b> | ||
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Revision as of 11:29, 25 September 2013
There are two solutions to \(ax^2 + bx + c = 0\) and they are
$$x = {-b \pm \sqrt{b^2-4ac} \over 2a}.$$
Bacterial Lab Protocols
Stable Transfection Of Adherent Cells
This protocol is for the stable transfection of eukaryotic adherent cell types in a 6-well plate, with plasmid DNA that ought to be of as high a purity as possible.
1) The day before transfection, seed 0.9-4x10^5 cell per well of the six well plate with 2ml of appropriate growth medium. This should produce a confluence of 40-80% for the next day's transfection.
2) Incubate cells in their normal growth conditions (37^0 C and 5% CO2).
3)
Mammalian Lab Protocols
expands with content
