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Template:Team:SydneyUni Australia/Calendar/Events List
From 2013.igem.org
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title: 'Gibson Assembly Take 2', | title: 'Gibson Assembly Take 2', | ||
start: new Date(2013, 7, 14), | start: new Date(2013, 7, 14), | ||
| - | end: new Date(2013, 7, 15) | + | end: new Date(2013, 7, 15), |
description: '<b>Members:</b> Rob, Hugh, Shuravi, Desmond<br> <b>What we did: </b> A few different things were tried including:<br><br>- Transforming into a different E. Coli host (TOP10), since perhaps our home-made cells weren’t up to IDT’s standards.<br><br>- Transforming into the same host (EPI300) but letting the cells recover and incubate at room temperature. Perhaps our pathway assembled correctly but overexpression of foreign enzymes impeded cells at maximum growth rate. Results were the same as the original Gibson Assembly transformation.' | description: '<b>Members:</b> Rob, Hugh, Shuravi, Desmond<br> <b>What we did: </b> A few different things were tried including:<br><br>- Transforming into a different E. Coli host (TOP10), since perhaps our home-made cells weren’t up to IDT’s standards.<br><br>- Transforming into the same host (EPI300) but letting the cells recover and incubate at room temperature. Perhaps our pathway assembled correctly but overexpression of foreign enzymes impeded cells at maximum growth rate. Results were the same as the original Gibson Assembly transformation.' | ||
}, | }, | ||
Revision as of 13:22, 25 September 2013
