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Template:Kyoto/Notebook/Aug 23
From 2013.igem.org
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Revision as of 15:29, 25 September 2013
Contents |
Aug 23
Electrophoresis
| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| 1 | pT181 antisense | EcoRI | SpeI |
| 2 | pT181 antisense | EcoRI | -- |
| 3 | pT181 antisense | -- | SpeI |
| 4 | pT181 antisense | -- | -- |
| 5 | pT181 antisense | XbaI | PstI |
| 6 | pT181 antisense | XbaI | -- |
| 7 | pT181 antisense | -- | PstI |
| 8 | pT181 antisense | -- | -- |
| 9 | 100bp ladder | -- | -- |
| 10 | Fusion1 attenuator | EcoRI | SpeI |
| 11 | Fusion1 attenuator | EcoRI | -- |
| 12 | Fusion1 attenuator | -- | SpeI |
| 13 | Fusion1 attenuator | -- | -- |
| 14 | Fusion1 attenuator | XbaI | PstI |
| 15 | Fusion1 attenuator | XbaI | -- |
| 16 | Fusion1 attenuator | -- | PstI |
| 17 | Fusion1 attenuator | -- | -- |
| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| 1 | Fusion3m2 attenuator | EcoRI | SpeI |
| 2 | Fusion3m2 attenuator | EcoRI | -- |
| 3 | Fusion3m2 attenuator | -- | SpeI |
| 4 | Fusion3m2 attenuator | -- | -- |
| 5 | Fusion3m2 attenuator | XbaI | PstI |
| 6 | Fusion3m2 attenuator | XbaI | -- |
| 7 | Fusion3m2 attenuator | -- | PstI |
| 8 | Fusion3m2 attenuator | -- | -- |
| 9 | 100bp ladder | -- | -- |
| 10 | tetR aptamer 12_1M | EcoRI | SpeI |
| 11 | tetR aptamer 12_1M | EcoRI | -- |
| 12 | tetR aptamer 12_1M | -- | SpeI |
| 13 | tetR aptamer 12_1M | -- | -- |
| 14 | tetR aptamer 12_P | EcoRI | SpeI |
| 15 | tetR aptamer 12_P | EcoRI | -- |
| 16 | tetR aptamer 12_P | -- | SpeI |
| 17 | tetR aptamer 12_P | -- | -- |
| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| 1 | Plac | SpeI | PstI |
| 2 | Plac | SpeI | -- |
| 3 | Plac | -- | PstI |
| 4 | Plac | -- | -- |
| 5 | Spinach | EcoRI | SpeI |
| 6 | Spinach | EcoRI | -- |
| 7 | Spinach | -- | SpeI |
| 8 | Spinach | -- | -- |
| 9 | 100bp ladder | -- | -- |
| 10 | J23100 | SpeI | PstI |
| 11 | J23100 | SpeI | -- |
| 12 | J23100 | -- | PstI |
| 13 | J23100 | -- | -- |
| 14 | tetR aptamer 12_1R | EcoRI | SpeI |
| 15 | tetR aptamer 12_1R | EcoRI | -- |
| 16 | tetR aptamer 12_1R | -- | SpeI |
| 17 | tetR aptamer 12_1R | -- | -- |
.jpg)
Restriction Enzyme Digestion
| Fusion6 antisense (1) | EcoRI | SpeI | buffer B | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 2 cuts | 3 | 0.5 | 0.5 | 3 | 0.3 | 22.7 | 30 |
| 1 cut | 0.6 | 0.1 | 0 | 1 | 0.1 | 8.2 | 10 |
| 1 cut | 0.6 | 0 | 0.1 | 1 | 0.1 | 8.2 | 10 |
| NC | 0.6 | 0 | 0 | 1 | 0.1 | 8.3 | 10 |
| Fusion6 antisense (1) | XbaI | PstI | buffer D | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 2 cuts | 3 | 0.5 | 0.5 | 3 | 0.3 | 22.7 | 30 |
| 1 cut | 0.6 | 0.1 | 0 | 1 | 0.1 | 8.2 | 10 |
| 1 cut | 0.6 | 0 | 0.1 | 1 | 0.1 | 8.2 | 10 |
| NC | 0.6 | 0 | 0 | 1 | 0.1 | 8.3 | 10 |
| Fusion1 antisense (1) | EcoRI | SpeI | buffer B | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 2 cuts | 3.3 | 0.5 | 0.5 | 3 | 0.3 | 22.4 | 30 |
| 1 cut | 0.7 | 0.1 | 0 | 1 | 0.1 | 8.1 | 10 |
| 1 cut | 0.7 | 0 | 0.1 | 1 | 0.1 | 8.1 | 10 |
| NC | 0.7 | 0 | 0 | 1 | 0.1 | 8.2 | 10 |
| pT181 antisense (1) | EcoRI | SpeI | buffer B | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 2 cuts | 5.6 | 0.5 | 0.5 | 3 | 0.3 | 20.1 | 30 |
| 1 cut | 1.1 | 0.1 | 0 | 1 | 0.1 | 7.7 | 10 |
| 1 cut | 1.1 | 0 | 0.1 | 1 | 0.1 | 7.7 | 10 |
| NC | 1.1 | 0 | 0 | 1 | 0.1 | 7.8 | 10 |
| pT181 antisense (1) | XbaI | PstI | buffer D | BSA | MilliQ | total | |
|---|---|---|---|---|---|---|---|
| 2 cuts | 5.6 | 0.5 | 0.5 | 3 | 0.3 | 20.1 | 30 |
| 1 cut | 1.1 | 0.1 | 0 | 1 | 0.1 | 7.7 | 10 |
| 1 cut | 1.1 | 0 | 0.1 | 1 | 0.1 | 7.7 | 10 |
| NC | 1.1 | 0 | 0 | 1 | 0.1 | 7.8 | 10 |
- incubate at 37°C from 17:29.
Electrophoresis
| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| 1 | 100bp | -- | -- |
| 2 | pT181 attenuator | EcoRI | SpeI |
| 3 | pT181 attenuator | XbaI | PstI |
| 4 | Fusion3m2 attenuator | EcoRI | SpeI |
| 5 | Fusion1 attenuator | XbaI | PstI |
| 6 | Fusion1 attenuator | EcoRI | SpeI |
| 7 | Fusion3m2 attenuator | XbaI | PstI |
| 8 | pT181 antisense | EcoRI | SpeI |
| 9 | pT181 antisense | XbaI | PstI |
| 10 | Fusion1 antisense | EcoRI | SpeI |
| 11 | Fusion1 antisense | XbaI | PstI |
| 12 | Fusion6 antisense | EcoRI | SpeI |
| 13 | Fusion6 antisense | XbaI | PstI |
| 14 | tetR aptamer 12_1M | EcoRI | SpeI |
| 15 | tetR aptamer 12_P | EcoRI | SpeI |
| 16 | tetR aptamer 12_1R | EcoRI | SpeI |
| 17 | 100bp | -- | -- |
.jpg)
| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| 1 | 100bp | -- | -- |
| 2 | Spinach | EcoRI | SpeI |
| 3 | J23100 | SpeI | PstI |
| 4 | Plac | SpeI | PstI |
| 5 | 100bp | -- | -- |
| 6 | J23100 | SpeI | PstI |
-2.jpg)
| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| 1 | 100bp | -- | -- |
| 2 | Fusion6 antisense | EcoRI | SpeI |
| 3 | Fusion6 antisense | EcoRI | -- |
| 4 | Fusion6 antisense | -- | SpeI |
| 5 | Fusion6 antisense | -- | -- |
| 6 | pT181 antisense | XbaI | PstI |
| 8 | 100bp | -- | -- |
| 9 | 100bp | -- | -- |
| 10 | Fusion6 antisense | XbaI | PstI |
| 11 | Fusion6 antisense | XbaI | -- |
| 12 | Fusion6 antisense | -- | PstI |
| 13 | Fusion6 antisense | -- | -- |
| 14 | pT181 antisense | XbaI | -- |
| 16 | 100bp | -- | -- |
| Lane | Sample | Enzyme1 | Enzyme2 |
|---|---|---|---|
| 1 | 100bp | -- | -- |
| 2 | Fusion1 antisense | EcoRI | SpeI |
| 3 | Fusion1 antisense | EcoRI | -- |
| 4 | Fusion1 antisense | -- | SpeI |
| 5 | Fusion1 antisense | -- | -- |
| 6 | pT181 antisense | -- | PstI |
| 8 | 100bp | -- | -- |
| 9 | 100bp | -- | -- |
| 10 | pT181 antisense | EcoRI | SpeI |
| 11 | pT181 antisense | EcoRI | -- |
| 12 | pT181 antisense | -- | SpeI |
| 13 | pT181 antisense | -- | -- |
| 14 | pT181 antisense | -- | -- |
| 16 | 100bp | -- | -- |
Gel Extraction
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | pT181 attenuator | XbaI & PstI |
| 3 | ||
| 5 | Fusion3m2 attenuator | EcoRI & SpeI |
| 6 | ||
| 8 | Fusion1 attenuator | XbaI & PstI |
| 9 | ||
| 11 | Fusion1 attenuator | EcoRI & SpeI |
| 12 |
-1.2.jpg)
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | Fusion3m2 attenuator | XbaI & PstI |
| 3 | ||
| 5 | pT181 antisense | EcoRI & SpeI |
| 6 | ||
| 8 | pT181 antisense | XbaI & PstI |
| 9 | ||
| 11 | Fusion1 antisense | XbaI & PstI |
| 12 |
File:Igku xxafterxx.xxx
File:Igku xxbeforexx.xxx
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | Fusion6 attenuator | XbaI & PstI |
| 3 | ||
| 5 | aptamer 12_1M | EcoRI & SpeI |
| 6 |
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | aptamer 12_P | EcoRI & SpeI |
| 3 | ||
| 5 | aptamer 12_1R | EcoRI & SpeI |
| 6 |
-2.4.jpg)
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 2 | Fusion6 antisense | EcoRI & SpeI |
| 3 | ||
| 5 | Fusion6 attenuator | XbaI & PstI |
| 6 | ||
| 8 | Fusion1 antisense | EcoRI & SpeI |
| 9 | ||
| 11 | pT181 antisense | EcoRI & SpeI |
| 12 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 3 | pT181 antisense | XbaI & PstI |
| 4 |
File:Igku xxbeforexx.xxx
File:Igku xxafterxx.xxx
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 3 | Plac | SpeI & PstI |
| 4 |
| Lane | DNA | Enzyme |
|---|---|---|
| 1 | 100bp ladder | -- |
| 3 | J23100 | SpeI & PstI |
| 5 | Spinach | EcoRI & SpeI |
| 6 |
