Template:Kyoto/Notebook/Aug 24

From 2013.igem.org

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|8/22 pT181 antisense (EcoRI & SpeI)||8||1.13||0.82
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|8/22 Fusion3m2 attenuator (XbaI & PstI)||5||2.277||1.50
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{| class="wikitable"
{| class="wikitable"
!Lane||Sample||Enzyme1||Enzyme2
!Lane||Sample||Enzyme1||Enzyme2
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Latest revision as of 16:18, 25 September 2013

Contents

Aug 24

Gel Extraction

Ashida

Nameconcentration[µg/mL]260/280260/230
8/22 aptamer 12_P (EcoRI & SpeI)8821.3--
8/22 aptamer 12_1R (EcoRI & SpeI)6323.1--
8/22 aptamer 12_1M (EcoRI & SpeI)4022.0--
8/23 Fusion6 antisense (EcoRI & SpeI)6.01.600.17
8/23 Fusion6 antisense (XbaI & PstI)6.01.710.25
8/23 pT181 antisense (EcoRI & SpeI)6.02.220.15
8/23 pT181 antisense (XbaI & PstI)6.02.100.04
8/22 Plac (SpeI & PstI)6.01.470.09
8/22 Fusion1 antisense (EcoRI & SpeI)102.160.01
8/22 J23100 (SpeI & PstI)6.01.520.35
Nameconcentration[µg/mL]260/280260/230
8/22 Spinach (EcoRI & SpeI)8.01.150.77
8/22 Fusion6 antisense (XbaI & PstI)441.070.96
8/22 Fusion1 antisense (XbaI & PstI)2--1.00
8/22 pT181 antisense (XbaI & PstI)3.2.010.40
8/22 pT181 antisense (EcoRI & SpeI)81.130.82
8/22 Fusion3m2 attenuator (XbaI & PstI)52.271.50
8/22 Fusion3m2 attenuator (EcoRI & SpeI)341.161.02
8/22 Fusion1 attenuator (XbaI & PstI)141.400.62
8/22 Fusion1 attenuator (EcoRI & SpeI)161.270.61
8/22 pT181 attenuator (XbaI & PstI)5--0.88
  • These samples were performed ethanol ethanl precipitation.

Restriction Enzyme Digestion

No name

tetR aptamer 12_P(2)EcoRISpeI10x Buffer B100x BSAMilliQtotal
2 cuts2.7µL0.5µL0.5µL3µL0.3µL23µL30µL
NC0.3µL0µL0µL1µL0.1µL8.6µL10µL
tetR aptamer 12_1R(2)EcoRISpeI10x Buffer B100x BSAMilliQtotal
2 cuts2.6µL0.5µL0.5µL3µL0.3µL23.1µL30µL
NC0.3µL0µL0µL1µL0.1µL8.6µL10µL
tetR aptamer 12_M(1)EcoRISpeI10x Buffer B100x BSAMilliQtotal
2 cuts2.7µL0.5µL0.5µL3µL0.3µL23µL30µL
NC0.3µL0µL0µL1µL0.1µL8.6µL10µL

Master Plate

No name

NumberUse LB plate(+Kan)
1entA- #1
2entA- #1
3entA- #1
4entA- #2
5entA- #2
6entA- #2

Electrophoresis

No name

LaneSampleEnzyme1Enzyme2
1100bp ladder--
2tetR aptamer 12_PEcoRISpeI
3tetR aptamer 12_P--
4tetR aptamer 12_1REcoRISpeI
5tetR aptamer 12_1R--
6tetR aptamer 12_1MEcoRISpeI
7tetR aptamer 12_1M--
8100bp ladder--

Igku Aug24 Electrophoresis(N1)-pic.jpg

LaneSampleEnzyme1Enzyme2
1100bp ladder--
2tetR aptamer 12_PEcoRISpeI
3tetR aptamer 12_1REcoRISpeI
4tetR aptamer 12_1MEcoRISpeI
5100bp ladder--


Igku Aug24 Electrophoresis(N2)-pic2.jpg


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