Team:SCUT

From 2013.igem.org

(Difference between revisions)

Revision as of 18:47, 25 September 2013

Abstract

E.cerevisiae is a sophisticated signal transport system between E.coli and S.cerevisiae. Producer, the E.coli, is assigned to distribute a special volatile—butanedione periodically with a stable oscillation circuit, which defines the meaning of the signal. On the other side, Sniffer，the yeast, transplanted with a nose from nematode, can respond to the signal immediately. We hope this can realize the communication between prokaryotes and eukaryotes for the further research on symbiosis.

Oscillating odorant contains two parts: the Producer and the Oscillator. We choose diacetyl as our desire odorant which widely used as a food additive to emit buttery flavor. In order to enhance the production, we construct the E.coli as the producer . Additionally, we add the oscillation system on the basis of odorant producing system, together, distributing a special volatile—butanedione periodically.		Receptor aims to fabricate abiomeric nose and mouth for S.cerevisiae to achieve the communication between eukaryotes and prokaryotes. We select a G protein coupled receptor(GPCR), odr-10, as our nose to detect diacetyl generated by E.coli In addition, we use aiiA gene as our mouth, which translate into aiiA enzyme secreted from S.cerevisiae with the help of signal peptide.
	各种文字

@@ Line 35: / Line 35: @@
 	 <img style="width:250px;height:420px;"src="https://static.igem.org/mediawiki/igem.org/0/0a/Scut_rightpart3.0.png"/>
 	 <div style="display:none;">
-	  <p class="r" style="">Receptor aims to fabricate abiomeric nose and mouth for S.cerevisiae to achieve the communication between eukaryotes and prokaryotes. We select a G protein coupled receptor(GPCR), odr-10, as our nose to detect diacetyl generated by E.coli In addition, we use aiiA gene as our mouth, which translate into aiiA enzyme secreted from S.cerevisiae with the help of signal peptide. The aiiA enzyme would inhibit the production of diacetyl, so that the E.coli and S.cerevisiae can communicate using languages diacetyl and aiiA enyme.</p>
+	  <p class="r" style="">Receptor aims to fabricate abiomeric nose and mouth for S.cerevisiae to achieve the communication between eukaryotes and prokaryotes. We select a G protein coupled receptor(GPCR), odr-10, as our nose to detect diacetyl generated by E.coli In addition, we use aiiA gene as our mouth, which translate into aiiA enzyme secreted from S.cerevisiae with the help of signal peptide.</p>
 	 </div>
 	</div>