Team:NJU China/Notebook

From 2013.igem.org

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<a>siRNA screening (Failed)</a>
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Revision as of 23:23, 25 September 2013

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Notebook

March

2013

Experiment training We learnt to culture the 293t cells. We learnt to extract plasmids and transfect it into 293t cells. We learnt to collect exosomes. We learnt to extract RNA from exosomes and cells. We learnt to do RT-PCR and qPCR.

April

2013

siRNA screening (Failed) 14th: We extracted plasmids and cultured the 293t cells. 15th: We transfected plasmids into 293t cells. 16th: We collected cells and preserved it in Trizol. 17th: We extracted RNA, then did RT-PCR with the RNA. 18th: We did qPCR with the cDNA we got on 17th April. 21th: We reexamined the concentration of RNA, and redid qPCR.

siRNA screening (Failed) 27th: We cultured the 293t cells in a 12-well plate. 28th: We transfected 293t cells. 29th: We collected cells and extracted RNA from it. 30th: We did RT-qPCR with the RNA we extracted on 29th April. 1st: We did qPCR with the cDNA we got on 30th April.

May

2013

siRNA screening (Failed) 27th: We cultured the 293t cells in a 12-well plate. 28th: We transfected 293t cells. 29th: We collected cells and extracted RNA from it. 30th: We did RT-qPCR with the RNA we extracted on 29th April. 1st: We did qPCR with the cDNA we got on 30th April.

August

2013

July

2013

June

2013

来源:站长素材