"
Team:NJU China/Notebook
From 2013.igem.org
(Difference between revisions)
| Line 676: | Line 676: | ||
<div class="ss-row ss-medium"> | <div class="ss-row ss-medium"> | ||
<div class="ss-left"> | <div class="ss-left"> | ||
| - | <a class="ss-circle ss-circle- | + | <a class="ss-circle ss-circle-4">Week4 (8th-13th, May)</a> |
</div> | </div> | ||
</div> | </div> | ||
| Line 682: | Line 682: | ||
<div class="ss-left"> | <div class="ss-left"> | ||
<h3> | <h3> | ||
| - | <a> siRNA screening (Failed)</a> | + | <a> siRNA screening (Failed), examination whether siRNA are capsulated into exosomes (Success)</a> |
| - | <span> | + | <span>8th: We extracted plasmids of 3 kinds of siRNA.</span> |
| - | <span> | + | <span>9th: We cultured 293t cells in eight D10 dishes and a 12-well plate.</span> |
| - | <span> | + | <span>10th: We extracted 2 kinds of over-expression plasmids and examined the concentration of it. Then we transfect these plasmids into 293t cells respectively.</span> |
| - | <span> | + | <span>11th: We collected cells and exosomes from 8 D10 dish, and cells from 12-well plate.</span> |
| - | <span> | + | <span>12th: We extracted RNA from cells and exosomes.</span> |
| + | <span>13th: We did RT-PCR and qPCR.</span> | ||
| + | |||
</h3> | </h3> | ||
</div> | </div> | ||
Revision as of 23:29, 25 September 2013
<!DOCTYPE html>
Notebook
March
2013
Experiment training We learnt to culture the 293t cells. We learnt to extract plasmids and transfect it into 293t cells. We learnt to collect exosomes. We learnt to extract RNA from exosomes and cells. We learnt to do RT-PCR and qPCR.
April
2013
siRNA screening (Failed) 14th: We extracted plasmids and cultured the 293t cells. 15th: We transfected plasmids into 293t cells. 16th: We collected cells and preserved it in Trizol. 17th: We extracted RNA, then did RT-PCR with the RNA. 18th: We did qPCR with the cDNA we got on 17th April. 21th: We reexamined the concentration of RNA, and redid qPCR.
siRNA screening (Failed) 27th: We cultured the 293t cells in a 12-well plate. 28th: We transfected 293t cells. 29th: We collected cells and extracted RNA from it. 30th: We did RT-qPCR with the RNA we extracted on 29th April. 1st: We did qPCR with the cDNA we got on 30th April.
May
2013
siRNA screening (Failed), examination whether siRNA are capsulated into exosomes (Success) 8th: We extracted plasmids of 3 kinds of siRNA. 9th: We cultured 293t cells in eight D10 dishes and a 12-well plate. 10th: We extracted 2 kinds of over-expression plasmids and examined the concentration of it. Then we transfect these plasmids into 293t cells respectively. 11th: We collected cells and exosomes from 8 D10 dish, and cells from 12-well plate. 12th: We extracted RNA from cells and exosomes. 13th: We did RT-PCR and qPCR.
September 27, 2013 Multi-level Photo Map
September 20, 2013 Responsive Image Gallery with Thumbnail Carousel
September 12, 2013 Elastislide - A Responsive jQuery Carousel Plugin
September 5, 2013 Slicebox - A fresh 3D image slider with graceful fallback
August
2013
August 23, 2013 Image Zoom Tour with jQuery
July
2013
July 29, 2013 Content Rotator with jQuery
July 5, 2013 Fullscreen Slideshow with HTML5 Audio and jQuery
June
2013
来源：站长素材
