"
Team:BYU Provo/Results/Modeling
From 2013.igem.org
| Line 40: | Line 40: | ||
Traditionally, experiments with bacteriophage growth is performed either in liquid broth or in solid agar medium. In contrast to the relative uniform distribution of phages in liquid broth, phages form individual clearings in bacterial lawn within solid agar medium. These clearings are termed plaques. They the product of bacterial cell lysis as phage replicate and spread outward. | Traditionally, experiments with bacteriophage growth is performed either in liquid broth or in solid agar medium. In contrast to the relative uniform distribution of phages in liquid broth, phages form individual clearings in bacterial lawn within solid agar medium. These clearings are termed plaques. They the product of bacterial cell lysis as phage replicate and spread outward. | ||
| - | + | produces individual clearings impose significant limitations on phage and bacterial movement. These restrictions allow phage with different characteristics to form different plaques. | |
We hypothesized that multiple experimental factors may influence | We hypothesized that multiple experimental factors may influence | ||
Revision as of 03:27, 26 September 2013
| |
|
|
Basic DesignTraditionally, experiments with bacteriophage growth is performed either in liquid broth or in solid agar medium. In contrast to the relative uniform distribution of phages in liquid broth, phages form individual clearings in bacterial lawn within solid agar medium. These clearings are termed plaques. They the product of bacterial cell lysis as phage replicate and spread outward. produces individual clearings impose significant limitations on phage and bacterial movement. These restrictions allow phage with different characteristics to form different plaques. We hypothesized that multiple experimental factors may influence
Many experimental A viral plaque is a visible structure formed within a cell culture, such as bacterial cultures within some nutrient medium (e.g. agar). The bacteriophage viruses replicate and spread, thus generating regions of cell destructions known as plaques. A plaque is developed as a part of infection of one cell by a single virus particle that is followed by the replication of that virus, and finally, the death of the cell. The newly replicated virus particles will later infect and then kill surrounding cells.
Cholera Team
|
