Team:UChicago/Protocols

From 2013.igem.org

(Difference between revisions)
Line 13: Line 13:
Protocols page
Protocols page
 +
 +
 +
Contents:
 +
 +
1 General Lab Best Practices
 +
 +
2 Lab Notebook Best Practices (read: do these best practices)
 +
 +
3 Steps to 3A Assembly & Labeling Guidelines
 +
 +
4 Resuspending DNA from iGEM Kit Plates
 +
 +
5 Recipes
 +
 +
:5.1 Making Agar Plates
 +
 +
:5.2 Pouring the Plates
 +
 +
:5.3 Making LB (500mL)     
 +
 +
6 Agar Stab Protocols
 +
 +
7 Ligation Protocol
 +
 +
8 Transformation Procedures
 +
 +
9 Making Overnight Cultures
 +
 +
10 Miniprep Protocols
 +
 +
11 Making an agarose gel for gel purification
 +
 +
12 Enzymatic digestion
 +
 +
13 Gel extraction with the QIAGEN kit
 +
{{:Team:UChicago/Templates/Standard_footer}}
{{:Team:UChicago/Templates/Standard_footer}}

Revision as of 03:28, 26 September 2013


Notebook > Protocols

Protocols page


Contents:

1 General Lab Best Practices

2 Lab Notebook Best Practices (read: do these best practices)

3 Steps to 3A Assembly & Labeling Guidelines

4 Resuspending DNA from iGEM Kit Plates

5 Recipes

5.1 Making Agar Plates
5.2 Pouring the Plates
5.3 Making LB (500mL)

6 Agar Stab Protocols

7 Ligation Protocol

8 Transformation Procedures

9 Making Overnight Cultures

10 Miniprep Protocols

11 Making an agarose gel for gel purification

12 Enzymatic digestion

13 Gel extraction with the QIAGEN kit


Retrieved from "http://2013.igem.org/Team:UChicago/Protocols"