Team:UChicago/Protocols

From 2013.igem.org

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Contents:
 
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1 General Lab Best Practices
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==General Lab Best Practices==
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2 Lab Notebook Best Practices (read: do these best practices)
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==Lab Notebook Best Practices (read: do these best practices)==
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3 Steps to 3A Assembly & Labeling Guidelines
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==Steps to 3A Assembly & Labeling Guidelines==
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4 Resuspending DNA from iGEM Kit Plates
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==Resuspending DNA from iGEM Kit Plates==
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5 Recipes
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==Recipes==
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:5.1 Making Agar Plates
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===Making Agar Plates===
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:5.2 Pouring the Plates
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===Pouring the Plates===
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:5.3 Making LB (500mL)    
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===Making LB (500mL)===   
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6 Agar Stab Protocols
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==Agar Stab Protocols==
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7 Ligation Protocol
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==Ligation Protocol==
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8 Transformation Procedures
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==Transformation Procedures==
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==Making Overnight Cultures==
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==Miniprep Protocols==
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==Making an agarose gel for gel purification==
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==Enzymatic digestion==
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==Gel extraction with the QIAGEN kit==
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9 Making Overnight Cultures
 
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10 Miniprep Protocols
 
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11 Making an agarose gel for gel purification
 
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12 Enzymatic digestion
 
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13 Gel extraction with the QIAGEN kit
 
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Revision as of 03:40, 26 September 2013


Notebook > Protocols

Protocols page


Contents

General Lab Best Practices

Lab Notebook Best Practices (read: do these best practices)

Steps to 3A Assembly & Labeling Guidelines

Resuspending DNA from iGEM Kit Plates

Recipes

Making Agar Plates

Pouring the Plates

Making LB (500mL)

Agar Stab Protocols

Ligation Protocol

Transformation Procedures

Making Overnight Cultures

Miniprep Protocols

Making an agarose gel for gel purification

Enzymatic digestion

Gel extraction with the QIAGEN kit