From 2013.igem.org
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| - | =='''6/28/13 Researching the Sequence of kerA'''==
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| - | *Planning subteams continued to order reagents and compile protocols.
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| - | *The plasmid design team decided on gibson assembly to produce the kerA biobrick with geneblocks ordered from IDT.
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| - | *kerA sequence was obtained from “Nucleotide Sequence and Expression of kerA, the Gene Encoding a Keratinolytic Protease of Bacillus licheniformis PWD-1” (Lin et al. 1995).
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| - | [[File:UChicago 2013 kerA plasmid design.png]]
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| - | *Two putative promoters (red), a possible ribosomal binding site (green), and a transcriptional terminator sequence (light blue, single underline) are indicated. The putative starting residues of the preprotein (pre = blue), proprotein (pro = pink), and mature protein (mature = purple) are indicated.
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| - | *EcoRI (orange) and PstI (blue) sites within the gene are indicated and must be removed to produce the kerA biobrick.
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Latest revision as of 08:15, 26 September 2013
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