"
Team:Macquarie Australia/Results
From 2013.igem.org
(Difference between revisions)
| Line 30: | Line 30: | ||
<h1><center><a class="four" a name="2"><b>Sequencing Results</h1></b></h1> | <h1><center><a class="four" a name="2"><b>Sequencing Results</h1></b></h1> | ||
| - | <center><h7> <p> | + | <center><h7> <p> Sequencing results obtained from our successful ligation of gBlock fragments</b></p></h7> |
<br> | <br> | ||
Revision as of 13:14, 26 September 2013
Results and Characterisation
This page aims to provide a summary of our most important successful results, which have provided large strides towards the develpment of goal; Chlorophyll synthesis within E. coli. An in-depth view of our results can be found in our Notebook
Primary Results
[Description]
Sequencing Results
Sequencing results obtained from our successful ligation of gBlock fragments
| BioBricks | Amino Acid Match | Match Percentage |
|---|---|---|
| POR | 317/319 | 99% |
| ChlG | 320/320 | 100% |
| ChlP | 421/427 | 99% |
| ChlM | 278/280 | 99% |
| YCF | 130/130 | 100% |
| CTH1 | 372/374 | 99% |
| ChlI1 | 353/353 | 100% |
| DVR1 | 299/300 | 99% |
| Plastocyanin | 97/97 | 100% |
| Gun4 | 216/216 | 100% |
Characterisation
Promoter was ligated onto the genes and plated up on LB + CAM plates, we observed a lot of colony growth. We inoculated LB + CAM broth with a colony from the coresponding plate. A variety of growth was observed, a few plates corresponding to a certain gense had a lot more colonies than others, this can be obesrved from the image with the description order of genes. More colonies coresponding to a gene displayed more colony growth than others which can be obesrved from the image description showing the order of genes on the agar.
