"
Team:Paris Saclay/Labwork
From 2013.igem.org
(Difference between revisions)
| Line 37: | Line 37: | ||
* Electrophoresis of the digestion of Bba_K1155000 by NotI, HpaI, MluI [[File:Pswrong.png|20px]] | * Electrophoresis of the digestion of Bba_K1155000 by NotI, HpaI, MluI [[File:Pswrong.png|20px]] | ||
* Extraction of Bba_K1155000 from DH5α | * Extraction of Bba_K1155000 from DH5α | ||
| + | |||
=='''Bba_K1155001'''== | =='''Bba_K1155001'''== | ||
| Line 45: | Line 46: | ||
* Denaturation of EcoRI/PstI used for the digestion of PCR products : BphR1 and PSB1C3 | * Denaturation of EcoRI/PstI used for the digestion of PCR products : BphR1 and PSB1C3 | ||
* Ligation of BphR1 and PSB1C3 | * Ligation of BphR1 and PSB1C3 | ||
| + | |||
| + | 07/10/13 | ||
| + | * Transformation of ligation of PSB1C3 and BphR1 [[File:Psright.png|20px]] | ||
| + | |||
=='''Bba_K1155002'''== | =='''Bba_K1155002'''== | ||
| Line 53: | Line 58: | ||
* Denaturation of EcoRI/PstI used for the digestion of PCR products : BphA1 and PSB1C3 | * Denaturation of EcoRI/PstI used for the digestion of PCR products : BphA1 and PSB1C3 | ||
* Ligation of BphA1 and PSB1C3 | * Ligation of BphA1 and PSB1C3 | ||
| + | |||
| + | 07/10/13 | ||
| + | * Transformation of ligation of PSB1C3 and BphA1 [[File:Psright.png|20px]] | ||
| + | |||
=='''Bba_K1155003'''== | =='''Bba_K1155003'''== | ||
| Line 68: | Line 77: | ||
* Digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI | * Digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI | ||
* Electrophoresis of the digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI [[File:Psright.png|20px]] | * Electrophoresis of the digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI [[File:Psright.png|20px]] | ||
| + | |||
=='''Bba_K1155004'''== | =='''Bba_K1155004'''== | ||
| + | |||
=='''Bba_K1155005'''== | =='''Bba_K1155005'''== | ||
| + | |||
=='''Bba_K1155006'''== | =='''Bba_K1155006'''== | ||
| + | |||
=='''Bba_K1155007'''== | =='''Bba_K1155007'''== | ||
| Line 92: | Line 105: | ||
07/10/13 | 07/10/13 | ||
* Transformation of Bba_I732019 in DH5α [[File:Pswrong.png|20px]] | * Transformation of Bba_I732019 in DH5α [[File:Pswrong.png|20px]] | ||
| + | |||
=='''Bba_K1155008'''== | =='''Bba_K1155008'''== | ||
| + | |||
=='''Bba_K1155009'''== | =='''Bba_K1155009'''== | ||
| Line 105: | Line 120: | ||
* Denaturation of EcoRI/PstI used for the digestion of PCR products : BphR2 and PSB1C3 | * Denaturation of EcoRI/PstI used for the digestion of PCR products : BphR2 and PSB1C3 | ||
* Ligation of BphR2 and PSB1C3 | * Ligation of BphR2 and PSB1C3 | ||
| + | |||
| + | 07/10/13 | ||
| + | * Transformation of ligation of PSB1C3 and BphR2 [[File:Psright.png|20px]] | ||
| + | |||
=='''PSB3K3'''== | =='''PSB3K3'''== | ||
Revision as of 15:32, 26 September 2013
Contents |
Labwork overview
Bba_K1155000
07/01/13
- Digestion of PSB1C3 plasmid and PCR products : Pfnr by EcoRI/ PstI
- Ligation of PSB1C3 and Pfnr
07/02/13
- Tranformation of Bba_K1155000 in DH5α
07/03/13
- Colony PCR of Bba_K1155000 to check good insertion of Pfnr in PSB1C3
- New culture of Bba_K1155000
07/04/13
- Estimation of Pfnr Colony PCR size fragments thanks to Gene manager
- Electrophoresis of Colony PCR products
- Stock of Bba_K1155000
- Culture of Bba_K1155000
- Extraction of Bba_K1155000 from DH5α
- Digestion of Bba_K1155000 by NotI, MluI, HpaI to check good insertion of Pfnr in PSB1C3 again
07/05/13
- Electrophoresis of the digestion of Bba_K1155000 by NotI, HpaI, MluI
- Extraction of Bba_K1155000 from DH5α
Bba_K1155001
07/09/13
- PCR purification of BphR1
- Digestion PCR products : BphR1 and digestion of PSB1C3
- Denaturation of EcoRI/PstI used for the digestion of PCR products : BphR1 and PSB1C3
- Ligation of BphR1 and PSB1C3
07/10/13
- Transformation of ligation of PSB1C3 and BphR1
Bba_K1155002
07/09/13
- PCR purification of BphA1
- Digestion PCR products : BphA1 and digestion of PSB1C3
- Denaturation of EcoRI/PstI used for the digestion of PCR products : BphA1 and PSB1C3
- Ligation of BphA1 and PSB1C3
07/10/13
- Transformation of ligation of PSB1C3 and BphA1
Bba_K1155003
07/02/13
- Tranformation of Bba_K592009 in DH5α
07/03/13
- New culture of Bba_K592009
07/05/13
- Extraction of Bba_K592009 from DH5α
07/08/13
- Digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI
- Electrophoresis of the digestion of Bba_K592009 by NotI, XhoI, EcoRI, EcoRI/PstI
Bba_K1155004
Bba_K1155005
Bba_K1155006
Bba_K1155007
07/02/13
- Tranformation of Bba_I732017 in DH5α
07/03/13
- New culture of Bba_I732017
07/05/13
- Extraction of Bba_I732017 from DH5α
07/08/13
- Digestion of Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
- Electrophoresis of the digestion of Bba_I732017 by NotI, XhoI, EcoRI, EcoRI/PstI
07/10/13
- Transformation of Bba_I732019 in DH5α
Bba_K1155008
Bba_K1155009
07/01/13
- Design of oligos for amplification of BphR2 gene of Pseudomonas pseudoalcaligenes, Pseudomonas oleovorans
07/09/13
- PCR purification of BphR2
- Digestion PCR products : BphR2 and digestion of PSB1C3
- Denaturation of EcoRI/PstI used for the digestion of PCR products : BphR2 and PSB1C3
- Ligation of BphR2 and PSB1C3
07/10/13
- Transformation of ligation of PSB1C3 and BphR2
PSB3K3
07/09/13
- Transformation of Bba_J04450 in DH5α
07/10/13
- Mini and maxi preparation of the culture of Bba_J04450
