Team:Groningen/Labwork/26 September 2013

From 2013.igem.org

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<h2>Mirjam</h2>
<h2>Mirjam</h2>
Checked the plates with MotB+S9+S5 and FliZ+S3+S5. These plates showed only red colonies.
Checked the plates with MotB+S9+S5 and FliZ+S3+S5. These plates showed only red colonies.
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<br>Made a restriction digestion of EstA, S3 and S9 with BamHI. Made a ligation between EstA and the S3 and S9 subunit.  
+
<br>Made a restriction digestion of EstA, S3 and S9 with BamHI. Made a ligation between EstA and the S3 and S9 subunit.
 +
<h2>Claudio & Sander</h2>
 +
First we now realize that iodine kills <i>Bacillus subtilis</i>.
 +
<br>6 colonies from each <i>Bacillus subtilis</i> transformation plate (BBa_K1085014-BBa_K1085018 and BBa_K1085014-BBa_K1085022) were inoculated in LB + Cm and were streaked out on LB agar + Cm + starch.
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<br>
 +
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<h2>Inne</h2>
 +
Ran a 0.8% agarose gel to check the results from the colony PCR, 12 samples were tested. mixture 5uL sample + 5uL 2x loading dye. gel ran at 90 Volts for 35 minutes.
 +
<br>
 +
Gel composition:
 +
<br>Gel 2
 +
<table border = "1">
 +
<td>Ladder</td>
 +
<td>1</td>
 +
<td>2</td>
 +
<td>3</td>
 +
<td>4</td>
 +
<td>5</td>
 +
<td>6</td>
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</tr>
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<tr>
 +
<td rowspan = "3"><br><br><br> </td>
 +
<td rowspan = "3"><br><br><br> </td>
 +
<td rowspan = "3"><br><br><br> </td>
 +
<td rowspan = "3"><br><br><br> </td>
 +
<td rowspan = "3"><br><br><br> </td>
 +
<td rowspan = "3"><br><br><br> </td>
 +
<td rowspan = "3"><br><br><br> </td>
 +
</tr>
 +
<tr>
 +
</tr>
 +
<tr>
 +
</tr>
 +
<tr>
 +
<td>Ladder</td>
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<td>7</td>
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<td>8</td>
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<td>9</td>
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<td>10</td>
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<td>11</td>
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<td>12</td>
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</tr>
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</table>
 +
 +
 +
<br>
</div>
</div>

Latest revision as of 17:09, 26 September 2013

Mirjam

Checked the plates with MotB+S9+S5 and FliZ+S3+S5. These plates showed only red colonies.
Made a restriction digestion of EstA, S3 and S9 with BamHI. Made a ligation between EstA and the S3 and S9 subunit.

Claudio & Sander

First we now realize that iodine kills Bacillus subtilis.
6 colonies from each Bacillus subtilis transformation plate (BBa_K1085014-BBa_K1085018 and BBa_K1085014-BBa_K1085022) were inoculated in LB + Cm and were streaked out on LB agar + Cm + starch.

Inne

Ran a 0.8% agarose gel to check the results from the colony PCR, 12 samples were tested. mixture 5uL sample + 5uL 2x loading dye. gel ran at 90 Volts for 35 minutes.
Gel composition:
Gel 2
Ladder 1 2 3 4 5 6





















Ladder 7 8 9 10 11 12