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'Lb13. Lc. lactis MG1363 pJP059: Good growth Lb16. Lc. lactis MG1363 noplasmid: Good growth Lb15. Lc. lactis MG1363-pGUS: Good growth 19. pSB4C5-CrtE~I /w RBS, Linker & ZF failed '+

'20. pSB4C5-CrtE~Z /w RBS, Linker & ZF failed 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed 75. pEL3A15 - CrtB failed ';

+

}

+

else if(id == 'd2013629')

+

{

+

ds = '<div id="dairy-text"><h1>Saturday 2013-06-29</h1> Name of participants: Magnus B, Alexander W, Nils A, Anders E,'+

+

'Mikael S, Lovisa P., Emil M., Peter C., Ken B.-A., Karl H. '+

+

'<h2>Ongoing constructs:</h2>'+

+

'L. reuteri: '+

+

'1. CF48-pLR581 2. 1063-pLUL631 3. DSM 20016-pVs2 4. 100-23-noplasmid 6. DSM 20016-pLUL63TsA8 7. DSM 20016-pGFP '+

+

'8. 100-23-pGT232 12. DSM 20016-noplasmid 14. DSM 20016-pLUL631(B?) L. plantarum: 5. 256-rifR-pAMβ1 '+

+

'10. 256-noplasmid 11. 36E-noplasmid '+

+

'E. faecalis: '+

+

'9. JH2-2 pAMβ1 '+

+

'L. lactis: '+

+

'13. MG1363-pJP059 15. unknown-pGus 16. MG1363-noplasmid '+

+

'E.coli (strain D5α): '+

+

'3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT '+

+

'19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF '+

+

'23. pSB1C3-CrtB 24.B0034-CrtI 38. pEL3A16-red 42. pSB4S15-red 43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL '+

+

'45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS 48. pSB1C3-B0034-His-STS 57.B0034-Idi 75. pEL3A15-CrtB '+

+

'76. pEL3K16-CrtI 60. PK401 dam 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 '+

+

'66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP '+

+

'71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP 77. pSB4C15 '+

+

'<h2>Todays work</h2>'+

+

'PCR purification: '+

+

'CP1 CP8 CP11 CP29 CP30 CP41 Cp44 '+

+

'Digestion: '+

+

'27. pEL3K16-red 51.pEL3C18-red 61. pSB1C3-CP1 62. pSB1C3-CP8 63. pSB1C3-CP11 64. pSB1C3-CP29 65. pSB1C3-CP30 '+

+

'66. pSB1C3-CP41 67. pSB1C3-Cp44 68. pSB3K3-CP1-B0032-BFP 69.pSB3K3-CP8-B0032-BFP 70. pSB3K3-CP11-B0032-BFP '+

+

'71. pSB3K3-CP29-B0032-BFP 72. pSB3K3-CP30-B0032-BFP 73. pSB3K3-CP41-B0032-BFP 74. pSB3K3-Cp44-B0032-BFP '+

+

'77. pSB4C15 (Assembly of 42. pSB4S15 and precut CmR) '+

+

'Ligation: '+

+

'24.3 B0034-CrtI -> 27. pEL3K16-red 23.2 pSB1C3-CrtB -> 38. pEL3A16-red '+

+

'Transformation: '+

+

'75. pEL3A15 – CrtB* 76. pEL3K16 – CrtI* *Cancelled, wrong solution was used -> redo tomorrow. '+

+

'Re-streak: '+

+

'1.2 pSB1C3-red (from frozen stock) 7. pSB3K3 (”) 60. PK401 dam '+

+

'Plasmid preparation: '+

+

'3. pSB3T5-red 4. pSB1A3-red 5. pSB4K15 7. pSB3K3-red 11. pSB4A15 42. pSB4S15-red '+

+

'O/N Screening PCR: '+

+

'25. pSB1C3-CrtY 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF 21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF '+

+

'(19,20,21(with DMSO dilution series(7,9,12)) '+

+

'PCR (screening?): '+

+

'57.B0034-Idi IspA '+

+

'Gel electrophoresis: '+

+

'43. pSB1C3-B0034-TAL 44. pSB1C3-B0034-His-TAL 45. pSB1C3-B0034-4CL 46. pSB1C3-B0034-His-4CL 47. pSB1C3-B0034-STS '+

+

'48. pSB1C3-B0034-His-STS 57.B0034-Idi IspA 19. pSB4C5-CrtE~I /w RBS, Linker & ZF 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF '+

+

'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF 38. pEL3A16-red (digest) 23.1 pSB1C3-CrtB (digest) 23.2 pSB1C3-CrtB (digest) '+

+

'27. pEL3K16-red (digest) 24.3 B0034-CrtI (digest) 24.4 B0034-CrtI (digest) '+

+

'<h2>Results</h2>'+

+

'Today: '+

+

'Plasmid preparation: '+

+

'42.1 pSB4S15-red klon 1: 27.4 ng/µL 42.2 pSB4S15-red klon 2: 21.8 ng/µL 42.3 pSB4S15-red klon 3: 24.3 ng/µL '+

+

'42.4 pSB4S15-red klon 4: 25.3 ng/µL '+

+

'Gel electrophoresis: '+

+

'43. pSB1C3-B0034-TAL: failed (image 1 2013.06.28) 44. pSB1C3-B0034-His-TAL: failed (“) 45. pSB1C3-B0034-4CL: failed (“) '+

+

'46. pSB1C3-B0034-His-4CL: failed (“) 47. pSB1C3-B0034-STS: failed (image 2 2013.06.28) 48. pSB1C3-B0034-His-STS: failed (“) '+

+

'57.B0034-Idi: still not good after three attempts IspA: eventually good 38. pEL3A16-red (digest): failed, redo subcloning tomorrow '+

+

'23.1 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow 23.2 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow '+

+

'27. pEL3K16-red (digest): succeded 24.3 B0034-CrtI (digest): failed, redo subcloning tomorrow '+

+

'24.4 B0034-CrtI (digest): failed, redo subcloning tomorrow '+

+

'Transformation: '+

+

'75. pEL3A15 - CrtB failed '+

+

'PCR purification (of promotors):* '+

+

'CP1: 40 ng/µl CP8: 41,3 ng/µl CP11: 46,7 ng/µl CP29: 40 ng/µl CP30: 58 ng/µl CP41: 41,9 ng/µl Cp44: 42,3 ng/µl '+

+

'*PCR-purification of the promotors may have washed away the promotors themselves, as they are almost as short as the primers that purification '+

+

'is meant to remove, on the other hand, the DNA-concentration of the purified product was measured to around 40 ng/µl. → redo all promotor '+

+

'constructs (not 77) from annealing of R and F (without PCR-cleaning). Everything left in PCR-machine, maybe the digested plasmids and '+

+

'B0032-BFP-parts can be used. '+

+

'Previous day: '+

+

'ON screening: '+

+

'19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions 20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions '+

+

'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions </div>';

}

else if(id == 'd201373')

@@ Line 233: / Line 233: @@
 'Lb13. Lc. lactis MG1363 pJP059: Good growth<br>Lb16. Lc. lactis MG1363 noplasmid: Good growth<br>Lb15. Lc. lactis MG1363-pGUS: Good growth<br><br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF failed<br>'+
   '20. pSB4C5-CrtE~Z /w RBS, Linker & ZF failed<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed<br>75. pEL3A15 - CrtB failed<br><br>';
+ }
+ else if(id == 'd2013629')
+ {
+ ds = '<div id="dairy-text"><h1>Saturday 2013-06-29</h1><br><p><b>Name of participants:</b> Magnus B, Alexander W, Nils A, Anders E,'+
+	'Mikael S, Lovisa P., Emil M., Peter C., Ken B.-A., Karl H.<br><br>'+
+	'<h2>Ongoing constructs:</h2>'+
+	'<b>L. reuteri:</b><br>'+
+	'1. CF48-pLR581<br>2. 1063-pLUL631<br>3. DSM 20016-pVs2<br>4. 100-23-noplasmid<br>6. DSM 20016-pLUL63TsA8<br>7. DSM 20016-pGFP<br>'+
+	'8. 100-23-pGT232<br>12. DSM 20016-noplasmid<br>14. DSM 20016-pLUL631(B?)<br><br><b>L. plantarum:</b><br>5. 256-rifR-pAMβ1<br>'+
+	'10. 256-noplasmid<br>11. 36E-noplasmid<br><br>'+
+	'<b>E. faecalis:</b><br>'+
+	'9. JH2-2 pAMβ1<br><br>'+
+	'<b>L. lactis:</b><br>'+
+	'13. MG1363-pJP059<br>15. unknown-pGus<br>16. MG1363-noplasmid<br><br>'+
+	'<b>E.coli (strain D5α):</b><br>'+
+	'3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br>15. pSB4K5-RBS-TAL-Linker-Zif268-RBS-4cl-Linker-PBSII-STS-OMT<br>'+
+	'19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>'+
+	'23. pSB1C3-CrtB<br>24.B0034-CrtI<br>38. pEL3A16-red<br>42. pSB4S15-red<br>43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>'+
+	'45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>48. pSB1C3-B0034-His-STS<br>57.B0034-Idi<br>75. pEL3A15-CrtB<br>'+
+	'76. pEL3K16-CrtI<br>60. PK401 dam<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>'+
+	'66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>'+
+	'71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>77. pSB4C15<br><br>'+
+	'<h2>Todays work</h2>'+
+	'<b>PCR purification:</b><br>'+
+	'CP1<br>CP8<br>CP11<br>CP29<br>CP30<br>CP41<br>Cp44<br><br>'+
+	'<b>Digestion:</b><br>'+
+	'27. pEL3K16-red<br>51.pEL3C18-red<br>61. pSB1C3-CP1<br>62. pSB1C3-CP8<br>63. pSB1C3-CP11<br>64. pSB1C3-CP29<br>65. pSB1C3-CP30<br>'+
+	'66. pSB1C3-CP41<br>67. pSB1C3-Cp44<br>68. pSB3K3-CP1-B0032-BFP<br>69.pSB3K3-CP8-B0032-BFP<br>70. pSB3K3-CP11-B0032-BFP<br>'+
+	'71. pSB3K3-CP29-B0032-BFP<br>72. pSB3K3-CP30-B0032-BFP<br>73. pSB3K3-CP41-B0032-BFP<br>74. pSB3K3-Cp44-B0032-BFP<br>'+
+	'77. pSB4C15 (Assembly of 42. pSB4S15 and precut CmR)<br><br>'+
+	'<b>Ligation:</b><br>'+
+	'24.3 B0034-CrtI -> 27. pEL3K16-red<br>23.2 pSB1C3-CrtB -> 38. pEL3A16-red<br><br>'+
+	'<b>Transformation:</b><br>'+
+	'75. pEL3A15 – CrtB*<br>76. pEL3K16 – CrtI* <br>*Cancelled, wrong solution was used -> redo tomorrow.<br><br>'+
+	'<b>Re-streak:</b><br>'+
+	'1.2 pSB1C3-red (from frozen stock)<br>7. pSB3K3 (”)<br>60. PK401 dam<br><br>'+
+	'<b>Plasmid preparation:</b><br>'+
+	'3. pSB3T5-red<br>4. pSB1A3-red<br>5. pSB4K15<br>7. pSB3K3-red<br>11. pSB4A15<br>42. pSB4S15-red<br><br>'+
+	'<b>O/N Screening PCR:</b><br>'+
+	'25. pSB1C3-CrtY<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>'+
+	'(19,20,21(with DMSO dilution series(7,9,12))<br><br>'+
+	'<b>PCR (screening?):</b><br>'+
+	'57.B0034-Idi<br>IspA<br><br>'+
+	'<b>Gel electrophoresis:</b><br>'+
+	'43. pSB1C3-B0034-TAL<br>44. pSB1C3-B0034-His-TAL<br>45. pSB1C3-B0034-4CL<br>46. pSB1C3-B0034-His-4CL<br>47. pSB1C3-B0034-STS<br>'+
+	'48. pSB1C3-B0034-His-STS<br>57.B0034-Idi<br>IspA<br>19. pSB4C5-CrtE~I /w RBS, Linker & ZF<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF<br>'+
+	'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF<br>38. pEL3A16-red (digest)<br>23.1 pSB1C3-CrtB (digest)<br>23.2 pSB1C3-CrtB (digest)<br>'+
+	'27. pEL3K16-red (digest)<br>24.3 B0034-CrtI (digest)<br>24.4 B0034-CrtI (digest)<br><br>'+
+	'<h2>Results</h2>'+
+	'<b>Today:</b><br>'+
+	'<b>Plasmid preparation:</b><br>'+
+	'42.1 pSB4S15-red klon 1: 27.4 ng/µL<br>42.2 pSB4S15-red klon 2: 21.8 ng/µL<br>42.3 pSB4S15-red klon 3: 24.3 ng/µL<br>'+
+	'42.4 pSB4S15-red klon 4: 25.3 ng/µL<br><br>'+
+	'<b>Gel electrophoresis:</b><br>'+
+	'43. pSB1C3-B0034-TAL: failed (image 1 2013.06.28)<br>44. pSB1C3-B0034-His-TAL: failed (“)<br>45. pSB1C3-B0034-4CL: failed (“)<br>'+
+	'46. pSB1C3-B0034-His-4CL: failed (“)<br>47. pSB1C3-B0034-STS: failed (image 2 2013.06.28)<br>48. pSB1C3-B0034-His-STS: failed (“)<br>'+
+	'57.B0034-Idi: still not good after three attempts<br>IspA: eventually good<br>38. pEL3A16-red (digest): failed, redo subcloning tomorrow <br>'+
+	'23.1 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>23.2 pSB1C3-CrtB (digest): failed, redo subcloning tomorrow<br>'+
+	'27. pEL3K16-red (digest): succeded <br>24.3 B0034-CrtI (digest): failed, redo subcloning tomorrow<br>'+
+	'24.4 B0034-CrtI (digest): failed, redo subcloning tomorrow<br><br>'+
+	'<b>Transformation:</b><br>'+
+	'75. pEL3A15 - CrtB failed<br><br>'+
+	'<b>PCR purification (of promotors):*</b><br>'+
+	'CP1: 40 ng/µl<br>CP8: 41,3 ng/µl<br>CP11: 46,7 ng/µl<br>CP29: 40 ng/µl<br>CP30: 58 ng/µl<br>CP41: 41,9 ng/µl<br>Cp44: 42,3 ng/µl<br><br>'+
+	'*PCR-purification of the promotors may have washed away the promotors themselves, as they are almost as short as the primers that purification '+
+	'is meant to remove, on the other hand, the DNA-concentration of the purified product was measured to around 40 ng/µl. → redo all promotor '+
+	'constructs (not 77) from annealing of R and F (without PCR-cleaning). Everything left in PCR-machine, maybe the digested plasmids and '+
+	'B0032-BFP-parts can be used.<br><br>'+
+	'<b>Previous day:</b><br>'+
+	'<b>ON screening:</b><br>'+
+	'19. pSB4C5-CrtE~I /w RBS, Linker & ZF: failed -> redo today with dilutions<br>20. pSB4C5-CrtE~Z /w RBS, Linker & ZF: failed -> redo today with dilutions<br>'+
+	'21. pSB4C5-CrtE~O-Z /w RBS, Linker & ZF failed: -> redo today with dilutions<br><br></p></div>';
   }
    else if(id == 'd201373')

Template:Team:Uppsala/JS/notebook

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