Team:NJU China/Notebook

From 2013.igem.org

(Difference between revisions)
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<html lang="en">
<html lang="en">
     <head>
     <head>
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}
}
.ss-circle-1{
.ss-circle-1{
-
     <P>WEEK</P>
+
     background-image: url(../images/1.jpg);
}
}
.ss-circle-2{
.ss-circle-2{
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<a href="#July">Jul</a>
<a href="#July">Jul</a>
<a href="#August">Aug</a>
<a href="#August">Aug</a>
-
                <a href="#September">Sep</a>
+
                                <a href="#September">Sep</a>
</div>
</div>
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             </div>
             </div>
         </div>
         </div>
-
        <script type="text/javascript" src="http://ajax.googleapis.com/ajax/libs/jquery/1.7.1/jquery.min.js"></script>
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*
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+
-
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*
+
-
* TERMS OF USE - jQuery Easing
+
-
*
+
-
* Open source under the BSD License.
+
-
*
+
-
* Copyright © 2008 George McGinley Smith
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-
* All rights reserved.
+
-
*
+
-
* Redistribution and use in source and binary forms, with or without modification,
+
-
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-
*
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* Redistributions of source code must retain the above copyright notice, this list of
+
-
* conditions and the following disclaimer.
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* Redistributions in binary form must reproduce the above copyright notice, this list
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* of conditions and the following disclaimer in the documentation and/or other materials
+
-
* provided with the distribution.
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*
+
-
* Neither the name of the author nor the names of contributors may be used to endorse
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+
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*
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-
* THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY
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* EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF
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* MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE
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-
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/*
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*
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* TERMS OF USE - EASING EQUATIONS
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*
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* Open source under the BSD License.
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*
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* Copyright © 2001 Robert Penner
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* All rights reserved.
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*
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* Redistribution and use in source and binary forms, with or without modification,
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*
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* of conditions and the following disclaimer in the documentation and/or other materials
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*
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*
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* THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" AND ANY
+
-
* EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF
+
-
* MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE
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-
*  COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL,
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*  NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED
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*
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-
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-
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+
-
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-
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+
-
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+
-
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+
-
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+
-
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+
-
+
-
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+
-
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-
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+
-
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+
-
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-
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+
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+
-
+
-
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+
-
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+
-
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+
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'-webkit-transform' : 'translate3d(-' + t + '%, 0, 0) rotateY(-' + r + 'deg) translate3d(-' + t + '%, 0, 0)',
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'opacity' : o
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$rowR.css({
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return { init : init };
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$sidescroll.init();
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Revision as of 18:36, 26 September 2013

 

Notebook

March

2013

Experiment training We learnt to culture the 293t cells. We learnt to extract plasmids and transfect it into 293t cells. We learnt to collect exosomes. We learnt to extract RNA from exosomes and cells. We learnt to do RT-PCR and qPCR.

April

2013

siRNA screening (Failed) 14th: We extracted plasmids and cultured the 293t cells. 15th: We transfected plasmids into 293t cells. 16th: We collected cells and preserved it in Trizol. 17th: We extracted RNA, then did RT-PCR with the RNA. 18th: We did qPCR with the cDNA we got on 17th April. 21th: We reexamined the concentration of RNA, and redid qPCR.

siRNA screening (Failed) 27th: We cultured the 293t cells in a 12-well plate. 28th: We transfected 293t cells. 29th: We collected cells and extracted RNA from it. 30th: We did RT-qPCR with the RNA we extracted on 29th April. 1st: We did qPCR with the cDNA we got on 30th April.

May

2013

siRNA screening (Failed), examination whether siRNA are capsulated into exosomes (Success) 8th: We extracted plasmids of 3 kinds of siRNA. 9th: We cultured 293t cells in eight D10 dishes and a 12-well plate. 10th: We extracted 2 kinds of over-expression plasmids and examined the concentration of it. Then we transfect these plasmids into 293t cells respectively. 11th: We collected cells and exosomes from 8 D10 dish, and cells from 12-well plate. 12th: We extracted RNA from cells and exosomes. 13th: We did RT-PCR and qPCR.

August

2013

July

2013

June

2013