Team:NJU China/Notebook

From 2013.igem.org

(Difference between revisions)
Line 607: Line 607:
                     </div>
                     </div>
                 </div>
                 </div>
-
                <div class="ss-row ss-medium">
+
<div class="ss-row ss-medium">
-
                    <div class="ss-left">
+
<div class="ss-left">
-
                        <h3>
+
 
-
<a>WEEK 1</a>
+
</div>
-
<span>25th-28th, March</span>
+
<div class="ss-right">
<h3>
<h3>
-
                    </div>
 
-
                    <div class="ss-right">
 
-
                        <h3>
 
<a>Experiment training</a>
<a>Experiment training</a>
<span>We learnt to culture the 293t cells.</span>
<span>We learnt to culture the 293t cells.</span>

Revision as of 18:44, 26 September 2013

 

Notebook

March

2013

Experiment training We learnt to culture the 293t cells. We learnt to extract plasmids and transfect it into 293t cells. We learnt to collect exosomes. We learnt to extract RNA from exosomes and cells. We learnt to do RT-PCR and qPCR.

April

2013

siRNA screening (Failed) 14th: We extracted plasmids and cultured the 293t cells. 15th: We transfected plasmids into 293t cells. 16th: We collected cells and preserved it in Trizol. 17th: We extracted RNA, then did RT-PCR with the RNA. 18th: We did qPCR with the cDNA we got on 17th April. 21th: We reexamined the concentration of RNA, and redid qPCR.

siRNA screening (Failed) 27th: We cultured the 293t cells in a 12-well plate. 28th: We transfected 293t cells. 29th: We collected cells and extracted RNA from it. 30th: We did RT-qPCR with the RNA we extracted on 29th April. 1st: We did qPCR with the cDNA we got on 30th April.

May

2013

siRNA screening (Failed), examination whether siRNA are capsulated into exosomes (Success) 8th: We extracted plasmids of 3 kinds of siRNA. 9th: We cultured 293t cells in eight D10 dishes and a 12-well plate. 10th: We extracted 2 kinds of over-expression plasmids and examined the concentration of it. Then we transfect these plasmids into 293t cells respectively. 11th: We collected cells and exosomes from 8 D10 dish, and cells from 12-well plate. 12th: We extracted RNA from cells and exosomes. 13th: We did RT-PCR and qPCR.

August

2013

July

2013

June

2013