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Team:NJU China/Notebook
From 2013.igem.org
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| - | <a> siRNA screening (Failed), examination whether siRNA are capsulated into exosomes (Success)</a> | + | <a>WEEK 4</a> |
| - | <span>8th: We extracted plasmids of 3 kinds of siRNA.</ | + | <span>8th-13th, May</span> |
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| - | + | <a>siRNA screening (Failed), examination whether siRNA are capsulated into exosomes (Success)</a> | |
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| - | + | 8th: We extracted plasmids of 3 kinds of siRNA.</br> | |
| - | + | 9th: We cultured 293t cells in eight D10 dishes and a 12-well plate.</br> | |
| + | 10th: We extracted 2 kinds of over-expression plasmids and examined the concentration of it. Then we transfect these plasmids into 293t cells respectively.</br> | ||
| + | 11th: We collected cells and exosomes from 8 D10 dish, and cells from 12-well plate.</br> | ||
| + | 12th: We extracted RNA from cells and exosomes.</br> | ||
| + | 13th: We did RT-PCR and qPCR.</br> | ||
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<a href="http://sc.chinaz.com/" class="ss-circle ss-circle-15">Scrollbar Visibility with jScrollPane</a> | <a href="http://sc.chinaz.com/" class="ss-circle ss-circle-15">Scrollbar Visibility with jScrollPane</a> | ||
Revision as of 19:09, 26 September 2013
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