Team:SCUT

From 2013.igem.org

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Revision as of 20:23, 26 September 2013

Abstract

E.cerevisiae is a sophisticated signal transport system between E.coli and S.cerevisiae. Producer, the E.coli, is assigned to distribute a special volatile—butanedione periodically with a stable oscillation circuit, which defines the meaning of the signal. On the other side, Sniffer，the yeast, transplanted with a nose from nematode, can respond to the signal immediately. We hope this can realize the communication between prokaryotes and eukaryotes for the further research on symbiosis.

Oscillating odorant contains two parts: the Producer and the Oscillator. We choose diacetyl as our desire odorant which widely used as a food additive to emit buttery flavor. In order to enhance the production, we construct the E.coli as the producer . Additionally, we add the oscillation system on the basis of odorant producing system, together, distributing a special volatile—butanedione periodically.

Receptor aims to fabricate biomimetic nose and mouth for S.cerevisiae to achieve the communication between eukaryotes and prokaryotes. We select a G-protein coupled receptor (GPCR), odr-10, as our nose to detect diacetyl generated by E.coli. In addition, we use aiiA gene as our mouth, which would translate into aiiA enzyme—an inhibitor to the production of diacetyl secreted from S.cerevisiae with the help of signal peptide.

The creation of our odorant producer and sensor presented us with interesting questions: Is the system feasible? And how fast would the sensing response be? Instead of costing too much time in the lab, we turned to modeling to give us an answer. We have built a kinetic model for our systems, diacetyl producer, oscillation and odr-10 pathway, to give us an estimate to observe a result.

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