Team:Alberta/MainSafety

From 2013.igem.org

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         <h3>Doesn’t <i>E. coli</i> cause food poisoning? If <i>E. coli</i> is so dangerous, why are they being used in labs?</h3>
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           <li>Normal <i>E. coli</i> colonize the lower intestine of humans, while <b>producing Vitamin K</b> and
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            <b>protecting infection</b> from other harmful bacteria.</li>
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          <li>Only some strains of <i>E. coli</i> cause food poisoning, but our group is using a <b>harmless,
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            nonpathogenic strain</b>. This train is engineered to contain mutations that would not allow the <i>E.
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            coli</i> to survive outside the lab without particular nutrients.</li>
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          <li><i>E. coli</i> provides a well-studied and well-known <b>model system</b> to test and troubleshoot new
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            trials on.</li>
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         <p>Reference: <a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1728375/">
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          http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1728375/</a></p>
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         <h3>What if plasmids containing <b>antibiotic resistance genes</b> were released into the environment despite
         <h3>What if plasmids containing <b>antibiotic resistance genes</b> were released into the environment despite
           precautions?</h3>
           precautions?</h3>

Revision as of 21:41, 26 September 2013


The Littlest Mapmaker

"Exploration into the world of DNA Computing"
Team Alberta: University of Alberta

Where to start?

What if plasmids containing antibiotic resistance genes were released into the environment despite precautions?

  • These genes are used in synthetic biology as selection tools.
  • There are lower chances of gene uptake by wild bacteria compared to lab-strain bacteria which have perforated membranes.
  • In the case that wild bacteria did uptake the antibiotic resistance gene, that gene would eventually be lost in the population without exposure to the antibiotic, because it is then a disadvantage for the bacteria to carry the unused plasmids. (Evidence observed in past with the decline of specific antibiotic resistance after the human population limited that antibiotic). Direct to subcategories