Team:Ciencias-UNAM/Project/GeneralDesign

From 2013.igem.org

(Difference between revisions)

Revision as of 22:34, 26 September 2013

General Design

HOME

PROJECT

HUMAN PRACTICES

TEAM

SAFETY

Overview

General Design

Wet Lab

Dry Lab

Conclusions

References

Bios

Sponsors

Overview

Journalism

Interviews

Home > Project > General Design

General Design

Our design has two systems:
The Antimicrobial Peptide System, which produces the peptide LL-37; and
The Resistance and Expulsion System which produces the MarA protein, which activates the arcAB & TolC operons, activating the arcAB & TolC efflux pump.

(aquí va la imagen)

Antimicrobial Peptide System

H. pylori and other pathogenic bacteria produce AI-2, a quorum sensing molecule. This signal is detected by the pLsrA promoter (BBa_K117002), which allows the transcription of LL37 (BBa_K1230001), which is traduced and then translated into the antimicrobial peptide LL37. Once LL37 is expelled by the ArcAB-TolC efflux pump, it can act against H. pylori.

Resitance & Expulsion System

This system is induced by promoter LacI (R0010), which allows the transcription of marA gene (BBa_K1230000), producing the protein MarA. MarA acts on the arcAB and tolC operons, which activates de ArcAB-TolC efflux pump, allowing the expulsion of LL37 peptide.

(Aquí va la imagen de la catrina)

Why Skully coli?

Mexican culture has an interesting relationship with death. There’s a holiday called The Day of the Death, (or Día de muertos in Spanish) in which the deceased are celebrated with colorfully decorated altars with sugar skulls, food, beverages and some of the favorite belongings of the deceased.

The protagonist of this celebration is Death itself. Death has different names (curiously enough, all of them feminine) which include: La Parca, La Huesuda, La Calaca, La Calavera and La Catrina (this last one is a name that Diego Rivera gave to a famous zinc etching by Jose Guadalupe Posada, portraying an “elegant skull”)

An interesting characteristic of La Catrina is that she serves as an intermediary in the death of a “chosen one”, meaning it only takes a specific victim. Our synthetic design intends to modify a bacteria so it can go after specific pathogenic bacteria in vivo. We though of the name Skully coli as a fun cultural analogy that also describes the intention of our system.

PROJECT MENU

Overview

General Design

Wet Lab

BioBricks

Results

Characterization

Protocols

Dry Lab

Modelling

Conclusions

Future Work

References

@@ Line 222: / Line 222: @@
 <span style="font-family:Arial;font-size:13px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">
-<i>H. pylori</i> and other pathogenic bacteria produce AI-2, a <i>quorum sensing</i> molecule. This signal is detected by the pLsrA promoter <a href=" http://parts.igem.org/wiki/index.php?title=Part:BBa_K117002">(BBa_K117002)</a>, which allows the transcription of LL37 (BBa_K1230001), which is traduced and then translated into the antimicrobial peptide LL37. Once LL37 is expelled by the ArcAB-TolC efflux pump, it can act against <i>H. pylori</i>.
+<i>H. pylori</i> and other pathogenic bacteria produce AI-2, a <i>quorum sensing</i> molecule. This signal is detected by the pLsrA promoter <a href=" http://parts.igem.org/wiki/index.php?title=Part:BBa_K117002">(BBa_K117002)</a>, which allows the transcription of LL37 <a href=" http://parts.igem.org/Part:BBa_K1230001">(BBa_K1230001)</a>, which is traduced and then translated into the antimicrobial peptide LL37. Once LL37 is expelled by the ArcAB-TolC efflux pump, it can act against <i>H. pylori</i>.
 <br>
 <span style="font-family:Museo Slab;font-size:16px;font-weight:normal;font-style:normal;text-decoration:none;color:#666666;">