Team:Carnegie Mellon/Project/Procedure

From 2013.igem.org

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<h1>Procedure</h1>
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[[image:Plasmid Construct.png|thumb|600px|center|The plasmid construct we use for expression consists of the <partinfo>BBa_R0010</partinfo> lac promoter, the <partinfo>BBa_B0034</partinfo> 100% efficiency RBS standard and either the KillerRed BBa_K1184000 coding sequence or mRFP1 <partinfo>BBa_E1010</partinfo> coding sequence. ]]
[[image:Plasmid Construct.png|thumb|600px|center|The plasmid construct we use for expression consists of the <partinfo>BBa_R0010</partinfo> lac promoter, the <partinfo>BBa_B0034</partinfo> 100% efficiency RBS standard and either the KillerRed BBa_K1184000 coding sequence or mRFP1 <partinfo>BBa_E1010</partinfo> coding sequence. ]]
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For a detailed description of our protocols and methods we used, visit our <a href="https://2013.igem.org/Team:Carnegie_Mellon/Protocols">Protocols</a> and <a href="https://2013.igem.org/Team:Carnegie_Mellon/Notebook">Notebook</a> pages.
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Revision as of 23:13, 26 September 2013

Killer Red



Procedure

The plasmid construct we use for expression consists of the <partinfo>BBa_R0010</partinfo> lac promoter, the <partinfo>BBa_B0034</partinfo> 100% efficiency RBS standard and either the KillerRed BBa_K1184000 coding sequence or mRFP1 <partinfo>BBa_E1010</partinfo> coding sequence.

For a detailed description of our protocols and methods we used, visit our Protocols and Notebook pages.