Team:Washington/M9Plate

From 2013.igem.org

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1. Make M9 + casamino acid media (link to protocol above)</br>
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1. Make <a href = "https://2013.igem.org/Team:Washington/M9Media">M9 + Casamino Acid Media</a></br>
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2. Add 1g or low melt agarose (http://www.lifetechnologies.com/order/catalog/product/16520050) per 100ml media. </br>
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2. Add 1g of <a href = "http://www.lifetechnologies.com/order/catalog/product/16520050">Low Melt Agarose</a> per 100ml media. </br>
3. Autoclave the mixture.</br>
3. Autoclave the mixture.</br>
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4. When solution has cooled down to 42 oC add antibiotic and innoculate.</br>
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4. When solution has cooled down to 42C add antibiotic and innoculate.</br>
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Revision as of 01:50, 27 September 2013



M9 + Casamino Acid Plate
1. Make M9 + Casamino Acid Media
2. Add 1g of Low Melt Agarose per 100ml media.
3. Autoclave the mixture.
4. When solution has cooled down to 42C add antibiotic and innoculate.

Retrieved from "http://2013.igem.org/Team:Washington/M9Plate"