Team:Washington/BlinkingTest

From 2013.igem.org

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1. Make <a href = "https://2013.igem.org/Team:Washington/M9Media">M9 + Casamino Acid Media</a></br>
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2. Add 1g of <a href = "http://www.lifetechnologies.com/order/catalog/product/16520050">Low Melt Agarose</a> per 100ml media. </br>
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1. Prepare 20 ml of <a href = "https://2013.igem.org/Team:Washington/M9Media">M9 + Casamino Acid Media</a> in a 50 ml falcon tube.</br>
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3. Autoclave the mixture.</br>
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2. Pipette 260 ul of inoculated M9 + casamino acid media into wells of interest.</br>
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4. When solution has cooled down to 42C add antibiotic and innoculate.</br>
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3. The blinking timer were set up as follows: </br>
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- always green light.</br>
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- 600 millisecond green wait and 400 millisecond black wait.</br>
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- 125 millisecond green wait and 875 millisecond black wait.</br>
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- 25 millisecond green wait and 975 millisecond black wait.</br>
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- 5 millisecond green wait and 995 millisecond black wait.</br>
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- always dark.</br>
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4. Follow steps of the <a href = "https://2013.igem.org/Team:Washington/96WellPlateAssay">96-Well Plate Assay</a>.
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Revision as of 03:00, 27 September 2013



M9 + Casamino Acid Plate
1. Prepare 20 ml of M9 + Casamino Acid Media in a 50 ml falcon tube.
2. Pipette 260 ul of inoculated M9 + casamino acid media into wells of interest.
3. The blinking timer were set up as follows:
- always green light.
- 600 millisecond green wait and 400 millisecond black wait.
- 125 millisecond green wait and 875 millisecond black wait.
- 25 millisecond green wait and 975 millisecond black wait.
- 5 millisecond green wait and 995 millisecond black wait.
- always dark.
4. Follow steps of the 96-Well Plate Assay.

Retrieved from "http://2013.igem.org/Team:Washington/BlinkingTest"